Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples

Authors

  • Özgür Çadırcı Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun
  • Ali Gücükoğlu Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun
  • Göknur Terzi Güzel Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun
  • Tolga Uyanık Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun
  • Abdulaziz Abdulahi Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Ondokuz Mayıs, 55200 Samsun
  • Mustafa Alişarlı Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Bölümü

DOI:

https://doi.org/10.24925/turjaf.v5i10.1142-1147.1228

Keywords:

MAP minced and cubed meat, Escherichia coli O157, H7, mPCR, Antimicrobial resistance

Abstract

Shiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present study were to detect E. coli O157 and O157: H7 strains from ground and cubed beef. A total of 100 MAP cattle meat products (50 minced meat, 50 meat cubes) were collected from the markets and butchers in Samsun province between May and October 2013. According to results, 1(1/50-2%) E. coli O157 and 1(1/50-2%) E. coli O157: H7 strains isolated from 50 ground beef samples, while 1 (1/50-2%) E. coli O157 strain was identified from 50 cubed beef samples. It was determined that E. coli O157 isolate obtained from the MAP ground beef carried stx1, stx2 genes; E. coli O157: H7 isolate carried stx1, stx2, eaeA and hylA genes while E. coli O157 isolate obtained from the MAP cubed meat only carried the stx2 gene. In antibiogram test, both E. coli O157 isolates were resistant to streptomycin and one E. coli O157: H7 isolate was resistant to streptomycin, cephalothin and tetracycline. As a consequence; in order to protect public health, products should be kept in proper hygienic and technical conditions during sale and storage and use of uncontrolled antibiotics should be avoided.

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Published

02.10.2017

How to Cite

Çadırcı, Özgür, Gücükoğlu, A., Terzi Güzel, G., Uyanık, T., Abdulahi, A., & Alişarlı, M. (2017). Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples. Turkish Journal of Agriculture - Food Science and Technology, 5(10), 1142–1147. https://doi.org/10.24925/turjaf.v5i10.1142-1147.1228

Issue

Section

Food Technologies