Determination of Polymorphisms in IGF-I and NPY Candidate Genes Associated with Egg Yield in Pure Layers Chicken Lines Reared in the Ankara Poultry Research Institute

Authors

  • Taki Karslı Akdeniz Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 07058 Antalya
  • Murat Soner Balcıoğlu Akdeniz Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 07058 Antalya
  • Eymen Demir Akdeniz Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 07058 Antalya
  • Hüseyin Göktuğ Fidan Akdeniz Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 07058 Antalya
  • Mehmet Aslan Akdeniz Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 07058 Antalya
  • Sedat Aktan Süleyman Demirel Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, 32260 Isparta
  • Serdar Kamanlı Ankara Tavukçuluk Araştırma Enstitüsü Müdürlüğü, 06170 Yenimahalle/Ankara
  • Kemal Karabağ Akdeniz Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, 07058 Antalya
  • Emine Şahin 5Akdeniz Üniversitesi, Korkuteli Meslek Yüksekokulu, 07800 Korkuteli, Antalya

DOI:

https://doi.org/10.24925/turjaf.v5i9.1051-1056.1290

Keywords:

Candidate genes, IGF-I, NPY, PCR-RFLP, Pure chicken lines

Abstract

Insulin-like factor-I (IGF-I) and neropeptide Y (NPY) are candidate genes related with reproductive traits in chicken. The aim of the present study, were to determine polymorphisms the IGF-I (5' untranslated region) and NPY genes in pure layer lines reared Ankara Poultry Research Institute by using PCR-RFLP method. For this purpose, 621 and 248 bp fragments were amplified for IGF-I and NPY genes, respectively. The PCR products were digested with PstI and DraI restriction enzymes, respectively, to detect of single nucleotide polymorphism (SNP) on IGF-I and NPY genes. All pure chicken lines were found polymorphic except Black and D-229 lines for the IGF-I gene. While the frequency of A allele ranged from 0.344 (COL) and 0.906 (RIRII) in brown layer pure lines for IGF-I gene, in white layer pure lines were found in the range between 0.781 (Maroon) and 1.000 (D-229, Brown). While all brown pure layer chicken lines were polymorphic for NPY gene in this research, only Maroon line was found monomorphic in white layer chicken lines. The frequency of the T allele for NPY gene ranged between 0.200 (BARI) and 0.985 (COL) in brown layers and between 0.397 (D-229) and 1.000 (Maroon) in white layers. According to applied chi-square test, no deviation from Hardy-Weinberg equilibrium was observed in polymorphic populations. As a result, polymorphisms were shown for the first time for IGF-I and NPY genes in pure layer lines reared at the Ankara Poultry Research Institute.

Published

12-09-2017

How to Cite

Karslı, T., Balcıoğlu, M. S., Demir, E., Fidan, H. G., Aslan, M., Aktan, S., Kamanlı, S., Karabağ, K., & Şahin, E. (2017). Determination of Polymorphisms in IGF-I and NPY Candidate Genes Associated with Egg Yield in Pure Layers Chicken Lines Reared in the Ankara Poultry Research Institute. Turkish Journal of Agriculture - Food Science and Technology, 5(9), 1051–1056. https://doi.org/10.24925/turjaf.v5i9.1051-1056.1290

Issue

Section

Animal Production

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