The Comparison of Various Real Time PCR Chemistries Used in Detection and Quantification of Genetically Modified Organisms

Authors

DOI:

https://doi.org/10.24925/turjaf.v7isp1.133-137.2782

Keywords:

Real-time PCR, Quantitative polymerase, Detection, GMOs q-PCR chemistries, Intercalation paints

Abstract

Real-time quantitative polymerase chain reaction (q-PCR) is an advanced molecular method for determining the amount of nucleic acid in both gene expression analysis and routine Deoxyribo Nucleic Acid (DNA) measurement. An accurate measurement method is essential given that the labelling threshold for genetically modified organisms (GMO) residues in food and feed products is 5% in Japan and 0,9% in the European Union. Determination of GMO components, quantification of exact amount and determination of trace amounts in food matrices are possible in q-PCR. Various q-PCR chemicals are used for this purpose. These; intercalation dyes, primary based, chemicals and probe based chemicals. With the increasing number of GMO products in the grocery stores, the number of analyses performed per sample and thus the cost of analysis increase. For this purpose, in GMO studies, improved detection methods are needed to determine the presence of GMOs in order to perform fast and economically feasible scans. In this study, q-PCR chemistries were compared in terms of cost, efficiency and applicability.

Author Biography

Leyla Bener, Institute of Science, Akdeniz University, 07058 Antalya

Agricultural Biotechnology Phd student

Published

10.12.2019

How to Cite

Bener, L., Ersal, M., & Yıldız, B. İ. (2019). The Comparison of Various Real Time PCR Chemistries Used in Detection and Quantification of Genetically Modified Organisms. Turkish Journal of Agriculture - Food Science and Technology, 7(sp1), 133–137. https://doi.org/10.24925/turjaf.v7isp1.133-137.2782