Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes

Authors

  • Celal Türker Osmaniye Korkut Ata University
  • Bahri Devrim Özcan Osmaniye Korkut Ata University

DOI:

https://doi.org/10.24925/turjaf.v3i6.387-393.312

Keywords:

Alpha-amylase, Bacillus sp., Isolation, Characterization, Thermophil

Abstract

In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis.

Published

07.03.2015

How to Cite

Türker, C., & Özcan, B. D. (2015). Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes. Turkish Journal of Agriculture - Food Science and Technology, 3(6), 387–393. https://doi.org/10.24925/turjaf.v3i6.387-393.312

Issue

Section

Animal Production