Changes in Total Phenolic and Protein Contents during Production of Protein Isolates from Carica papaya (Pawpaw) Seeds

Authors

  • Oseni Kadiri Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,
  • Saka O Gbadamosi Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,
  • Babatunde Olawoye Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,
  • Charles T Akanbi Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,

DOI:

https://doi.org/10.24925/turjaf.v4i11.1006-1013.550

Keywords:

Polyphenols, Carica papaya seed, Total phenolic content, Protein, Pearson Correlation test, Processing

Abstract

The effect of different processing stages- defatting, solubilization, isoelectric precipitation, neutralisation and lyophilisation on the total phenolic content and protein content of Carica papaya (pawpaw) seeds (CPS) full-fat flour (CFF), defatted flour (CDF), protein concentrates (CPC) and protein isolates (CPI) was studied. The folin-ciocalteu procedure was used to assess the total phenolic concentrations of the Carica papaya products and results were expressed as gallic acid equivalent (GAE). Optimal protein content was estimated at pH 10 for all samples. Higher levels of polyphenols were extracted using water-methanol (20/80) mixture compared to 100% methanol. Total phenolic content (TPC) was demonstrated in a trend of CDF > CPC > CFF > CPI with CDF having the highest TPC. The finding suggested a more economical protein isolate production method. The study concludes that the processing of CPS into the various products (CFF, CDF, CPC & CPI) improves its protein and polyphenol content.

Author Biographies

Oseni Kadiri, Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,

Dept. of Food Science and Technology Research Assitant

Saka O Gbadamosi, Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,

Food Science and Technology/ Senior Lecturer(Dr)

Charles T Akanbi, Department of Food Science and Technology, Obafemi Awolowo University, PMB 13, Ile-Ife,

Food Science and Technology/ Professor

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Published

19.11.2016

How to Cite

Kadiri, O., Gbadamosi, S. O., Olawoye, B., & Akanbi, C. T. (2016). Changes in Total Phenolic and Protein Contents during Production of Protein Isolates from Carica papaya (Pawpaw) Seeds. Turkish Journal of Agriculture - Food Science and Technology, 4(11), 1006–1013. https://doi.org/10.24925/turjaf.v4i11.1006-1013.550

Issue

Section

Food Technologies