Investigation of Regeneration in Colchicum soboliferum via Somatic Embryogenesis Method
DOI:
https://doi.org/10.24925/turjaf.v13i4.1002-1007.7420Keywords:
Colchicum sobolifera, somatic embryogenesis, in vitro, regenerationAbstract
In this study, the regeneration of the Colchicum soboliferum species, one of the medicinal aromatic plants growing naturally in Türkiye, was examined by the somatic embryogenesis method. Within the scope of experiments on somatic embryogenesis, corms of the Colchicum soboliferum species were used as explant source. 16 media containing 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1, 2 mg.L⁻¹), Benzyl adenine (BA) (0, 0.1, 0.5 mg.L⁻¹), and 2-Isopentenyladenine (2IP) (0, 0.1, 0.5 mg.L⁻¹) as plant growth regulators were used in this regeneration research. Different ratios and combinations of Murashige ve Skoog (MS) media were tested. In the applications, the highest embryogenic callus formation was observed with a rate of 60% in Murashige ve Skoog (MS) media containing 0.5 mg.L⁻¹ 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0.1 mg.L⁻¹ 2-Isopentenyladenine (2IP). The highest embryo formation, with a rate of 48.33%, was obtained in MS media containing 2 mg.L⁻¹ 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0.5 mg.L⁻¹ Benzyl adenine (BA) and 2 mg.L⁻¹ 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0.1 mg.L⁻¹ 2-Isopentenyladenine (2IP). No growth was observed in the control application that did not contain plant growth regulators.
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