Influence of Dietary Black Pepper Meal Supplementation on the Haemato-Serological Profile of West African Dwarf Goats
DOI:
https://doi.org/10.24925/turjaf.v13i7.1737-1744.7519Keywords:
Blood, Health, Immunity, Phytogenic additives, SupplementAbstract
Phytogenic feed additives, such as black pepper meal (BPM), show promise for improving animal health and productivity. However, its application in ruminant nutrition remains underexplored. Thus, a 56-day study was conducted to assess the effect of BPM supplementation on nutrient intake, haematological, and serum biochemical indices in West African Dwarf (WAD) goats. A basal diet was formulated and divided into four equal portions. Each portion was supplemented with BPM at 0 (control), 250, 500, and 750 mg and designated as Diet A, B, C and D, respectively. Twenty WAD goats (average liveweight 5.00±0.23 kg) were assigned to the four dietary treatments. Blood samples were collected for haematological while serum harvested were used for serum biochemical analyses. Dietary DM, CP and NFE slightly decreased with increased BPM supplementation. DM intake significantly increased from 312.28±12.09 g/day (Diet A) to 316.88±14.74 g/day (Diet D) (p<0.05). Crude protein and fiber intake increased slightly. It is noteworthy that supplementation of BPM did not significantly influence the observed haematological parameters (p>0.05). Serum biochemical indices did not follow a particular trend. However, goats fed Diet B had significantly higher total protein (72.00±4.83 g/L) and globulin (34.00±3.09 g/L) compared to other treatments (p<0.05). The study revealed that use of BPM supplementation in goats’ diets may not be essential, however, supplementation up to 750 mg/100 kg (Diet D) may support intake and thus, enhance immune functions, positioning BPM as additive to improve animal’ health without adverse effect.
References
Abdelnour, S., Alagawany, M., Abd El-Hack, M. E., Sheiha, A. M., Saadeldin, I. M., & Swelum, A. A. (2018). Growth, carcass traits, blood hematology, serum metabolites, immunity, and oxidative indices of growing rabbits fed diets supplemented with red or black pepper oils. Animals, 8(10), 168. https://doi.org/10.3390/ani8100168
Abou-Elkhair, R., Ahmed, H. A., & Selim, S. (2014). Effects of black pepper (Piper nigrum), turmeric powder (Curcuma longa) and coriander seeds (Coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens. Asian-Australasian Journal of Animal Sciences, 27(6), 847. https://doi.org/10.5713/ajas.2013.13644
Alade, C. T., Ibhaze, G. A., Osho, T. S., & Fajemisin, A. N. (2023). Impact of roasting on nutritional composition of some underutilized tropical seeds in ruminant nutrition. In Agricultural revolution for sustainable livelihood. (Edited) Dr. OM Akinnagbe and Dr. LO Oparinde. Proceedings of 11th Annual Conference of FUTA AGRIC CONFERENCE (pp. 8-11).
AOAC (2011) Official methods of analysis of AOAC International. 18th edn, Revision 4, In: Horwitz W, Latimer GW Jr (eds) AOAC International. Gaithersburg, Maryland, USA.
Arilekolasi, T. A., Omotoso, O. B., Faluyi, O. B., Fajemisin, A. N., & Alokan, J. A. (2020). Dietary effect of with or without molasses in rice husk based-diet on performance and health status of goats. Archiva Zootechnica, 23(1), 22-33. https://doi.org/10.2478/azibna-2020-0003
Ayeni, A., Oso, T., Olowoyeye, J., & Popoola, O. (2024). Performance characteristics, haematology and serological properties of broiler chickens fed diets supplemented with Talinum triangulare. Slovak Journal of Animal Science, 57(3). https://doi.org/10.36547/sjas.894
Bermúdez, J. H., Castellote, J. L. B., & Rodríguez, C. C. (2020). Relevance of the study of metabolic profiles in sheep and goat flock. Present and future: A review. Spanish Journal of Agricultural Research, 18(3), 12. https://doi.org/10.5424/sjar/2020183-14627
Clement, B. A., Goff, J. M., & Forbes, J. A. (2010). Blood biochemical profiles and health indicators in poultry systems. Journal of Veterinary Medicine, 60(2), 52- 59.
Dacie, J.V. & Lewis, S.M. (1991). Practical Textbook of Haematology. 7th Edition, Church Livingstone, Edinburgh, United Kingdom.
Daniel, O. A. (2015). Urban extreme weather: a challenge for a healthy Living environment in Akure, Ondo State, Nigeria. Climate, 3(4), 775-791. https://doi.org/10.3390/cli3040775
Eke, S. S., Omalu, I. C. J., Ochaguba, J. E., Urama, A. C., Hassan, S. C., Otuu, C. A., & Okafor, I. D. (2019). Prevalence of gastrointestinal parasites of sheep and goats slaughtered in Minna Modern Abattoir, Niger State, Nigeria. Journal of Animal Science and Veterinary Medicine, 4(2), 65-70. https://doi.org/10.31248/jasvm2019.131
Galib, M. A., Ahmed, M. H., & Haroon, M. R. (2011). Dietary hot red pepper and its effect on white blood cell count in broilers. Journal of Poultry Science, 3(2), 120-127.
Garg, A. P. (2023). Biomedical Applications of Black Pepper, the King of Spices-A Review. Biomedical Journal of Scientific and Technical Research, 53(1), 44364-44371. https://doi.org/10.26717/bjstr.2023.53.008353
Gross, D., & Tolba, R. H. (2015). Ethics in animal-based research. European Surgical Research, 55(1-2), 43-57. https://doi.org/10.1159/000377721
Herdt, T. H., & Hoff, B. (2011). The use of blood analysis to evaluate trace mineral status in ruminant livestock. Veterinary Clinics: Food Animal Practice, 27(2), 255-283. https://doi.org/10.1016/j.cvfa.2011.02.004
Hurrell, R. F., Reddy, M., & Cook, J. D. (1999). Inhibition of non-haem iron absorption in man by polyphenolic-containing beverages. British Journal of Nutrition, 81(4), 289-295. https://doi.org/10.1017/s0007114599000537
Ibhaze, G. A., & Fajemisin, A. N. (2015). Feed intake and nitrogen metabolism by West African Dwarf does fed naturally fermented maize cob-based diets. World Journal of Animal Science Research, 3(2), 1-8. https://doi.org/10.1186/s42269-021-00519-9
Ibhaze, G. A., Osilaja, T. O., Oso, T. S., Alade, C. T., & Ogunjemite, G. E. (2024). Nutrient, antinutrient intake, and digestibility of West African Dwarf does fed sole diet containing wheat offal substituted with tiger nut. Annals of Animal and Biological Research, 4(1).
Jesuyon, O. M., Boluwaji, O., Orunmuyi, M., Aganga, A. A., & Ogunjimi, S. I. (2023). Assessment of Management and Breeding Practices among Indigenous Goat Farmers in a Tropical Humid Forest Zone. In Goat Science-Environment, Health and Economy. IntechOpen. https://doi.org/10.5772/intechopen.99141
Kalio, G. A., Ayuk, A. A., & Agwunobi, L. N. (2014). Performance of West African Dwarf (WAD) goats fed N-treated source and forage supplemented cassava peels in humid Cross River State, Nigeria. American Journal of Experimental Agriculture, 4(6), 629-638. https://doi.org/10.9734/ajea/2014/6972
Mamoon, R. (2008). Goats and their nutrition. Manitoba Agriculture, Food and Rural Initiatives: Management of Smallholder-Owned Sheep and Goats by Utilizing Local Resources. Proceedings of the Second DFID Livestock Production Programme Link Project (R7798) Workshop for Small Stock Keepers, Sokoine University of Agriculture, pp. 69-75.
Melillo, A. (2013). Serum proteins and their role in avian health. Journal of Veterinary Research, 44(4), 303-315.
NRC (2007). Nutrient Requirement of Small Ruminants. National Academies Press, Washington DC, USA.
Omotoso, O. B., Noah, F. A., & Adebayo, A. J. (2019). Nitrogen Metabolism, Digestibility and blood profile of West African dwarf goats fed dietary levels of Cajanus cajan as supplement to cassava peels. Journal of Rangeland Science, 9(1), 13-23.
Omotoso, O. B., Ogunshola, O. J., Omoleye, S. O., & Alokan, J. A. (2017). Haematological and Serum Biochemical responses of West African Dwarf Goats Fed Panicum maximum replaced with untreated cocoa pod husk meal. Animal Research International, 14(3), 2826-2835. http://www.zoo-unn.org/index.php/ARI/article/view/574/pdf
Opara, M.N., Udevi, N. & Okoli, I.C. (2010). Hematological parameters and blood chemistry of apparently-healthy West African Dwarf (WAD) goats in Owerri, South Eastern Nigeria. New York Science Journal, 3: 68-72.
Oppong, E. N. V., sTimon, V. M., & Baber, R. P. (1988). Health control for sheep and goats in the humid tropics of West Africa.
Oso, T. S., Alade, C. T., Ibhaze, G. A., & Faluyi, O. B. (2024). Nutrient digestibility, Nitrogen utilization, and growth performance of West African Dwarf (WAD) does fed diets containing wheat offal replaced with Tiger nut seed meal. Archiva Zootechnica 27(2), 137-154. https://doi.org/10.2478/azibna-2024-0019
Plumb, D.C. (2018). Plumb's Veterinary Drug Handbook: Pocket. 9th Edition, John Wiley and Sons, USA. https://books.google.com.ng/books?hl=en&lr=&id=vshQDwAAQBAJ&oi=fnd&pg=PA1&ots=0gSbgsX3E4&sig=bq5uvnbGiC-pgSM52Ir517OOXtU&redir_esc=y#v=onepage&q&f=false
Puvača, N., Ljubojević, D., Lukač, D., Kostadinović, L., Stanaćev, V., Popović, S., Baloš, M.Ž. & Nikolova, N. (2014). Digestibility of fat in broiler chickens influenced by dietary addition of spice herbs. Macedonian Journal of Animal Science, 4(2). https://doi.org/10.54865/mjas1442061p
Reitman, S. & Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1): 56 – 63. https://doi.org/10.1093/ajcp/28.1.56
Rezende, M. M., Lemos, F. S., & Dourado, L. R. (2017). Serum protein levels as indicators of dietary protein utilization in poultry. Journal of Animal Science, 95(8), 3699- 3709
Ryan, K., Bain, B. J., Worthington, D., James, J., Plews, D., Mason, A., Roper, D., Rees, D. C., De La Salle, B., & Streetly, A. (2010). Significant haemoglobinopathies: Guidelines for screening and diagnosis. British Journal of Haematology, 149(1), 35–49. https://doi.org/10.1111/j.1365-2141.2009.08054.x
Soltan, Y. A., & Patra, A. K. (2021). Ruminal microbiome manipulation to improve fermentation efficiency in ruminants. Animal Feed Science and Nutrition – Production, Health and Environment, 1-20. DOI: 10.5772/intechopen.101582.
Srinivasan, K. (2016). 1034 Metabolic effects of dietary pungent spices on the gut in animal models. Journal of Animal Science, 94(suppl_5), 495-496. https://doi.org/10.2527/jam2016-1034
MSD Veterinary Manual. (2022a). Hematology reference ranges. Retrieved January 25, 2025, from https://www.msdvetmanual.com/reference-values-and-conversion-tables/reference-guides/hematology-reference-ranges
MSD Veterinary Manual. (2022b). Serum biochemical analysis reference ranges. Retrieved January 25, 2025, from https://www.msdvetmanual.com/reference-values-and-conversion-tables/reference-guides/clinical-chemistry-si-conversion-factors
U.S. Department of Agriculture (2009). USDA Food Data Central Spices, Pepper, Black Available at https://fdc.nal.usda.gov/fdc-app.html#/food-details/170931/nutrients. Accessed December 27 2024.
Van Soest, P. V., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. https://doi.org/10.3168/jds.s0022-0302(91)78551-2
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