Investigation of β-Lactam Antibiotic Residues in Cow’s Milk Collected from Ardahan Province
DOI:
https://doi.org/10.24925/turjaf.v13i6.1521-1525.7572Keywords:
Ardahan, Milk, Cattle, Antibiotic, ResidueAbstract
Milk and dairy products constitute essential nutritional sources for humans, particularly infants and children. From a public health perspective, it is therefore imperative that animal-derived milk remains free from residues that may pose risks to human health. This study aims to assess the presence of beta-lactam antibiotic residues in bovine milk. Between July and August 2023, a total of 180 raw cow’s milk samples were collected from six distinct locations (Ardahan, Göle, Çıldır, Hanak, Damal, and Posof) within the Ardahan region. The samples were obtained from milk collection tanks and cattle farms, with 15 samples collected from each location. Approximately 30 ml of milk per sample was placed in sterile plastic containers with lids, immediately transported to the laboratory, and stored at -20°C until analysis. The detection of beta-lactam antibiotic residues in the milk was performed using the ELISA method. The analysis confirmed the absence of beta-lactam antibiotic residues in all 180 raw cow’s milk samples. Consequently, this study indicates that raw cow’s milk from the Ardahan region is safe for human consumption. To ensure food safety and protect public health, it is recommended that training sessions be conducted to emphasise the importance of responsible antibiotic use and strict adherence to legal withdrawal periods for antibiotic-treated cattle. Additionally, the regular and systematic monitoring of antibiotic residues in milk is strongly advised.
References
Abdel-Galil, N. M., Ahmed, A. A.-H., Saad, N. M., & Amin, W. F. (2025). Risk assessment of oxytetracycline and penicillin residues in raw milk. Assiut Veterinary Medical Journal, 71(184), 13–28. https://doi.org/10.21608/avmj.2024.318055.1384
Acaroz, U., İnce, S., Arslan-Acaroz, D., Kucukkurt, I., & Eryavuz, A. (2020). Determination of kanamycin residue in anatolian buffalo milk by LC-MS/MS. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(1), 97-102. https://doi.org/10.9775/kvfd.2019.22401
Albanna, A., Fadhel, M. N., & Al-Shaker, Y. M. S. (2020). Detection Beta-lactam residues in imported and local dairy products in Mosul city. IOP Conference Series: Earth and Environmental Science, 553(1), 012014. https://doi.org/10.1088/1755-1315/553/1/012014
Anonim. (2017). Türk Gıda Kodeksi: Hayvansal gıdalarda bulunabilecek farmakolojik aktif maddelerin sınıflandırılması ve maksimum kalıntı limitleri yönetmeliği. Gıda Tarım ve Hayvancılık Bakanlığı. Resmi Gazete (07.03.2017, Sayı: 30000). [Erişim 29 Ocak 2025]. https://www.resmigazete.gov.tr/eskiler/2017/03/20170307-4.htm
Ardahan İl Tarım ve Orman Müdürlüğü. (2025). 2025 yılı Ocak brifingi. T.C. Tarım ve Orman Bakanlığı. [Erişim 9 Şubat 2025]. https://ardahan.tarimorman.gov.tr/Menu/77/Brifing
Ardiç, M., & Durmaz, H. (2006). Investigation of beta-lactam residues in unpacked milk consumed in Sanlıurfa. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 1(3–4), 74–77.
Aycan, E., & İnce, S. (2018). Presence of beta-lactam antibiotic residues in raw milk obtained from Afyonkarahisar Province. Kocatepe Veterinary Journal, 11(2), 113–118. https://doi.org/10.30607/kvj.376452
Ayvazoğlu, C., Akyüz, E., Kiziltepe, Ş., & Gökçe, G. (2021). The prevalence of enzootic bovine leukosis in cattle in Ardahan region. Journal of Advances in VetBio Science and Techniques, 7(1), 1–7. https://doi.org/10.31797/vetbio.952470
Ercan, N., & Ağaoğlu, S. (2022). Investigation of beta-lactam group antibiotics residue in raw cow milk in Sivas Province. Turkish Journal of Agriculture - Food Science and Technology, 10(12), 2373–2376. https://doi.org/10.24925/turjaf.v10i12.2373-2376.5451
Ergin Kaya, S., & Filazi, A. (2010). Determination of antibiotic residues in milk samples. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16, 31–35. https://doi.org/10.9775/kvfd.2009.1174
Ghanavi, Z., Mollayi, S., & Eslami, Z. (2013). Comparison between the amount of penicillin G residue in raw and pasteurized milk in Iran. Jundishapur Journal of Microbiology, 6(7), e12724. https://doi.org/10.5812/jjm.12724
Gritli, A., Teber, Y., Lahmar, R., & Youssef, S. Ben. (2021). Beta-lactam antibiotics residues in milk samples collected in Northern Tunisia. International Journal of Food Science and Agriculture, 5(3), 550–554. https://doi.org/10.26855/ijfsa.2021.09.027
Ibraimi, Z., Shehi, A., Hajrulai, Z., Mata, E., & Murtezani, A. (2013). Detection and risk assessment of beta-lactam residues in Kosovo’s milk using Elisa method. International Journal of Pharmacy and Pharmaceutical Sciences, 5(4), 446–450.
Khaskheli, M., Malik, R. S., Arain, M. A., Soomro, A. H., & Arain, H. H. (2008). Detection of β - Lactam antibiotic residues in market milk. Pakistan Journal of Nutrition, 7(5), 682–685. https://doi.org/10.3923/pjn.2008.682.685
Khmaissa, M., Zouari-Mechichi, H., Sciara, G., Record, E., & Mechichi, T. (2024). Pollution from livestock farming antibiotics an emerging environmental and human health concern: A review. Journal of Hazardous Materials Advances, 13, 100410. https://doi.org/10.1016/j.hazadv.2024.100410
Mangsi, A. S., Khaskheli, M., Soomro, A. H., & Shah, M. G. (2014). Detection of antimicrobial drug residues in milk marketed at different areas of Sindh province. IOSR Journal of Agriculture and Veterinary Science, 7(5), 65–69. https://doi.org/10.9790/2380-07546569
Mimoune, N., Seddiki, S., Baazizi, R., Saboundji, I. E., Saidi, R., Khelef, D., & Kaidi, R. (2021). Antibiotic residues in cow’s milk. Veterinarska Stanica, 52(5), 499–509. https://doi.org/10.46419/vs.52.5.9
Mortaş, H., Navruz Varlı, S., & Bilici, S. (2022). Sokak sütlerinde gizli tehlike: Antibiyotik kalıntısı. Journal of Nutrition and Dietetics, 50(1), 19–26. https://doi.org/10.33076/2022.bdd.1551
Movassagh, M. H., & Karami, A. R. (2011). Determination of beta lactam antibiotics residues in cow raw milk by beta star test. Global Veterinaria, 6(4), 366–368.
Oruç, H. H., & Sonal, S. (2005). Determination of oxytetracycline, penicillin G and sulphadimidine residues in cow milks in Bursa. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 24(1-2-3–4), 11–13.
Rahman, M. S., Hassan, M. M., & Chowdhury, S. (2021). Determination of antibiotic residues in milk and assessment of human health risk in Bangladesh. Heliyon, 7(8), e07739. https://doi.org/10.1016/j.heliyon.2021.e07739
Shi, R., Yu, Z., Wu, W., Ho, H., Wang, J., Wang, Y., & Han, R. (2020). A survey of 61 veterinary drug residues in commercial liquid milk products in China. Journal of Food Protection, 83(7), 1227–1233. https://doi.org/10.4315/JFP-20-048
Temamoǧullari, F., & Kaya, S. (2010). Determination of various antibiotic residues in milk samples sold in Ankara markets by thin layer chromotography and bioautographic method. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(2), 187–191. https://doi.org/10.9775/kvfd.2009.416
Tuncay, R. M., Sancak, Y. C., Çakmak, T., & Işleyici, Ö. (2022). Van’da tüketime sunulan çiğ sütlerde mikrobiyolojik kalite ve antibiyotik varlığı. Manas Journal of Agriculture Veterinary and Life Sciences, 12(1), 53–63. https://doi.org/10.53518/mjavl.1033318
Zavaleta-Monestel, E., Webster, K., Rojas-Chinchilla, C., Muñoz-Gutierrez, G., & Díaz-Madriz, J. P. (2024). Management and implications of beta-lactam allergies. Cureus, 16(5), e60281. https://doi.org/10.7759/cureus.60281
Zhang, W. Q., Yu, Z. N., Ho, H., Wang, J., Wang, Y. T., Fan, R. B., & Han, R. W. (2020). Analysis of veterinary drug residues in pasteurized milk samples in Chinese milk bars. Journal of Food Protection, 83(2), 204–210. https://doi.org/10.4315/0362-028X.JFP-19-333
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