Leptin Levels at Different Stages of the Luteal Phase in Cattle and Their Relationship with Conception
DOI:
https://doi.org/10.24925/turjaf.v13i7.1815-1819.7583Keywords:
Cattle, Conception, Leptin, Luteal PhaseAbstract
The objective of this study was to evaluate the relationship between plasma leptin levels in the early and late luteal periods of cows and conception rate. In the present study, the ovsynch + progesterone (P4) protocol was employed in 50 Holstein cows. Blood samples were collected on the 5th and 18th days following artificial insemination, and the levels of leptin and progesterone were subsequently measured. Subsequent to artificial insemination, mean leptin levels in the early (day 5) and late (day 18) luteal phases of the cows were measured as 5.96 ± 2.54 and 8.02 ± 4.14 ng/mL, respectively. Levels of leptin in the blood of pregnant cows on both day 5 (9.01 ± 4.40 ng/mL) and day 18 (12.04 ± 6.49 ng/mL) were higher than those in the blood of non-pregnant cows (4.44 ± 3.83 ng/mL and 6.01 ± 4.93 ng/mL, respectively). The findings revealed that progesterone levels on the 5th day following artificial insemination exhibited higher mean values (2.11 ± 0.64 ng/mL) in pregnant cows as compared to non-pregnant cows (1.49 ± 0.51 ng/mL). Furthermore, a higher level of progesterone was observed on day 18 in pregnant cows (8.89 ± 2.01 ng/mL) in comparison to non-pregnant cows (5.22 ± 2.21 ng/mL) (p<0.05). The study revealed that levels of the hormones leptin and progesterone were elevated in pregnant cows during the early and late luteal phase, in comparison to non-pregnant cows. However, no correlation was determined between P4 and leptin. The conclusion drawn was that, while there was a possibility that leptin levels could have an effect on the process of pregnancy and implantation, no relationship was found between progesterone and leptin levels
References
Arias-Álvarez, M., Bermejo-Alvarez, P., Gutierrez-Adan, A., Rizos, D., Lorenzo, P. L., & Lonergan, P. (2011). Effect of leptin supplementation during in vitro oocyte maturation and embryo culture on bovine embryo development and gene expression patterns. Theriogenology, 75(5), 887-896. https://doi.org/10.1016/j.theriogenology.2010.10.031
Barash, I. A., Cheung, C. C., Weigle, D. S., Ren, H., Kabigting, E. B., Kuijper, J. L., Clifton, D. K., & Steiner, R. A. (1996). Leptin is a metabolic signal to the reproductive system. Endocrinology, 137(7), 3144-3147. https://doi.org/10.1210/en.137.7.3144
Barbato, O., Terzano, G. M., Brecchia, G., Todini, L., Canali, C., & Barile, V. L. (2013). Leptin and pregnancy: preliminary results in buffalo cows (Bubalus bubalis). Vol.32 (Special Issue 2): 505-508.
Blache, D., Tellam, R. L., Chagas, L. M., Blackberry, M. A., Vercoe, P. E., & Martin, G. B. (2000). Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep. Journal of Endocrinology, 165(3), 625-637. https://doi.org/10.1677/joe.0.1650625
Block, S. S., Smith, J. M., Ehrhardt, R. A., Diaz, M. C., Rhoads, R. P., Van Amburgh, M. E., & Boisclair, Y. R. (2003). Nutritional and developmental regulation of plasma leptin in dairy cattle. Journal of dairy science, 86(10), 3206-3214. https://doi.org/10.3168/jds.S0022-0302(03)73923-X
Dotsch, J., Nusken, K. D., Knerr, I., Kirschbaum, M., Repp, R., & Rascher, W. (1999). Leptin and neuropeptide Y gene expression in human placenta: ontogeny and evidence for similarities to hypothalamic regulation. The Journal of Clinical Endocrinology & Metabolism, 84(8), 2755-2758. https://doi.org/10.1210/jcem.84.8.5892
Ehrhardt, R. A., Bell, A. W., & Boisclair, Y. R. (2002). Spatial and developmental regulation of leptin in fetal sheep. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 282(6), 1628-1635. https://doi.org/10.1152/ajpregu.00750.2001
Gentry Jr, G. T., Gentry, L. R., & Godke, R. A. (2013). The effect of exogenous follicle stimulating hormone (FSH) and endogenous plasma leptin concentrations on the pregnancy rate of beef heifers subjected to fixed-timed artificial insemination (FTAI). Animal reproduction science, 138(1-2), 49-54. https://doi.org/10.1016/j.anireprosci.2013.01.018
Grattan, D. R., Ladyman, S. R., & Augustine, R. A. (2007). Hormonal induction of leptin resistance during pregnancy. Physiology & behavior, 91(4), 366-374. https://doi.org/10.1016/j.physbeh.2007.04.005
Henson, M. C., & Castracane, V. D. (2006). Leptin in pregnancy. Biology of reproduction, 74(2), 218-229. https://doi.org/10.1095/biolreprod.105.045120
Herrid, M., Palanisamy, S. K., Ciller, U. A., Fan, R., Moens, P., Smart, N. A., & McFarlane, J. R. (2014). An updated view of leptin on implantation and pregnancy: a review. Physiological research, 63(5), 543-557. https://doi.org/10.33549/physiolres.932674
Hoggard, N., Haggarty, P., Thomas, L., & Lea, R. G. (2001). Leptin expression in placental and fetal tissues: does leptin have a functional role? Biochemical Society Transactions, 29(2), 57-63. https://doi.org/10.1042/bst0290057
Jéquier, E., & Tappy, L. (1999). Regulation of body weight in humans. Physiological reviews, 79, 451–480. https://doi.org/10.1152/physrev.1999.79.2.451
Kivrak, M. B., Turk, S., Takci, A., Bolukbas, B., Agaoglu, R. T., & Coskun, A. (2023). Pulse wave Doppler ultrasound of umbilical cord in experimentally induced pregnancy toxemia in sheep. Research in Veterinary Science, 160, 18-25. https://doi.org/10.1016/j.rvsc.2023.05.004
Kızıl, M., Rişvanlı, A., Abay, M., Şafak, T., Kılınç, M. A., Yılmaz, Ö., & Şeker, İ. (2023). Effect of calf delivery mode on İrisin, Asprosin, leptin, adiponectin, and İnsulin-like growth Factor-1 levels in dairy cattle and their calves. Pakistan Journal of Zoology, 55(4), 1527-1535. https://dx.doi.org/10.17582/journal.pjz/20221031081040
Malik, N. M., Carter, N. D., Murray, J. F., Scaramuzzi, R. J., Wilson, C. A., & Stock, M. J. (2001). Leptin requirement for conception, implantation, and gestation in the mouse. Endocrinology, 142(12), 5198-5202. https://doi.org/10.1210/endo.142.12.8535
Pérez-Pérez, A., Sánchez-Jiménez, F., Maymó, J., Dueñas, J. L., Varone, C., & Sánchez-Margalet, V. (2015). Role of leptin in female reproduction. Clinical Chemistry and Laboratory Medicine (CCLM), 53(1), 15-28. https://doi.org/10.1515/cclm-2014-0387
Priyadarshini, L., Yadav, A. K., Singh, H. S., Mishra, A., Jain, A. K., & Ahirwar, M. K. (2015). Role of leptin in physiology of animal reproduction-A review. Agricultural Reviews, 36(3), 235-240. http://dx.doi.org/10.5958/0976-0741.2015.00027.6
Risvanli, A., Kizil, M., Kilinc, M. A., Şafak, T., Yilmaz, Ö., Yüksel, B. F., & Seker, İ. (2022). Luteal Kistli Simental Irkı İneklerde Kan Adipokin, Antioksidan ve Biyokimyasal Parametre Düzeylerinin Belirlenmesi. Firat Universitesi Saglik Bilimleri Veteriner Dergisi, 36(2), 128-133.
Sansinanea, A. S., Cerone, S. I., Zonco, I., Garcı́a, C., & Auza, N. (2001). Serum leptin levels in cattle with different nutritional conditions. Nutrition Research, 21(7), 1045-1052. https://doi.org/10.1016/S0271-5317(01)00307-4
Schwartz, M. W., Seeley, R. J., Campfield, L. A., Burn, P., &
Baskin, D. G. (1996). Identification of targets of leptin action in rat hypothalamus. The Journal of clinical investigation, 98(5), 1101-1106. https://doi.org/10.1172/JCI118891
Sinha, M. K., & Caro, J. F. (1998). Clinical aspects of leptin. Vitamins & Hormones, 54, 1-30. https://doi.org/10.1016/S0083-6729(08)60919-X
Smith, G. D., Jackson, L. M., & Foster, D. L. (2002). Leptin regulation of reproductive function and fertility. Theriogenology, 57(1), 73-86. https://doi.org/10.1016/S0093-691X(01)00658-6
Smolinska, N., Kaminski, T., Siawrys, G., & Przala, J. (2013). Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs. Animal, 7(1), 109-117. https://doi.org/10.1017/S1751731112001103
Thomas, L., Wallace, J. M., Aitken, R. P., Mercer, J. G., Trayhurn, P., & Hoggard, N. (2001). Circulating leptin during ovine pregnancy in relation to maternal nutrition, body composition and pregnancy outcome. Journal of Endocrinology, 169(3), 465-476. https://doi.org/10.1677/joe.0.1690465
Troisi, A., Cardinali, L., Menchetti, L., Speranza, R., Verstegen, J. P., & Polisca, A. (2020). Serum concentrations of leptin in pregnant and non‐pregnant bitches. Reproduction in Domestic Animals, 55(4), 454-459. https://doi.org/10.1111/rda.13637
Weigle, D. S. (1997, January). Leptin and other secretory products of adipocytes modulate multiple physiological functions. In Annales d'endocrinologie, 58(2), 132-136.
Wylie, A. R. G. (2011). Leptin in farm animals: where are we and where can we go? Animal, 5(2), 246-267. https://doi.org/10.1017/S1751731110001540
Zerani, M., Boiti, C., Dall’Aglio, C., Pascucci, L., Maranesi, M., Brecchia, G., Mariottini, C., Guelfi, G., Zampini D., & Gobbetti, A. (2005). Leptin receptor expression and in vitro leptin actions on prostaglandin release and nitric oxide synthase activity in the rabbit oviduct. Journal of endocrinology, 185(2), 319-325. https://doi.org/10.1677/joe.1.05983
Zerani, M., Boiti, C., Zampini, D., Brecchia, G., Dall’Aglio, C., Ceccarelli, P., & Gobbetti, A. (2004). Ob receptor in rabbit ovary and leptin in vitro regulation of corpora lutea. Journal of Endocrinology, 183(2), 279-288. https://doi.org/10.1677/joe.1.05507
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