The Level of Hydroxymethylfurfural (HMF) in Strained Flower Honey Offered for Consumption in Sivas Province and Its Impact on Public Health

Authors

DOI:

https://doi.org/10.24925/turjaf.v13i6.1543-1549.7612

Keywords:

Honey, Hydroxymethylfurfural (HMF), Food safety, Public health, Monitoring, Quality

Abstract

This study aimed to determine the levels of Hydroxymethylfurfural (HMF), determined using a spectrophotometric method, in strained floral honey samples available for consumption in Sivas province with a focus on public health. Out of 50 honey samples obtained from national brands and local producers, 10% (n = 5) exceeded the permissible limit of <40 mg/kg imposed by the Turkish Food Codex Communiqué on Honey. The HMF concentrations in these samples were measured as 40.08, 61.55, 67.79, 132.28 and 240.48 mg/kg, for which the latest one obtained from a local producer. The occurrence of high HMF levels in honey samples indicates a potential threat to health due to prolonged exposure. In this regard, the study contributes significantly to determining the HMF concentration of strained floral honey available for consumption, as well as assessing its effects on the rights of consumers and public health. To mitigate potential health risks, further risk assessment studies should be conducted, food safety regulations should be re-evaluated, and educational campaigns targeting honey producers should be implemented.

References

Abraham, K., Gürtler, R., Berg, K., Heinemeyer, G., Lampen, A., & Appel, K. E. (2011). Toxicology and risk assessment of 5‐Hydroxymethylfurfural in food. Molecular nutrition & food research, 55(5), 667-678. https://doi.10.1002/mnfr.201000564

Afshari, A., Ram, M., & Mohamadi, S. (2022). Quality evaluation of Iranian honey collected from Khorasan province, Iran. International Journal of Food Science, 2022(1), 3827742. https://doi.org/10.1155/2022/3827742

Ağaoğlu, S., Şahin, S., & Demir, T. (2021). Fonksiyonel Gıda Olarak Arı Ürünleri: Özellikleri ve Sağlık Üzerindeki Etkileri. Evreklioğlu C. (Ed.), Sağlık Bilimlerinde Araştırma ve Değerlendirmeler -IV (pp. 27-42), Gece Kitaplığı, Ankara.

Akdeniz, G., Şahin, S., Yılmaz, Ö., Karataş, Ü., Karmaz, E., Kabakçı, D., & Yaşar, N. (2013). Karaçalı (Paliurus spina-christi Miller) ve ayçiçeği (Helianthus annuus L.) ballarının mikroskobik yapısı ve biyokimyasal özelliklerinin karşılaştırılması. Arıcılık Araştırma Dergisi, 5(9), 22-25.

Alqarni, A. S., Owayss, A. A., & Mahmoud, A. A. (2016). Physicochemical characteristics, total phenols and pigments of national and international honeys in Saudi Arabia. Arabian Journal of Chemistry, 9(1), 114-120. https://doi.org/10.1016/j.arabjc.2012.11.013

Althaiban, M. A. (2024). Investigation of hydroxymethylfurfural levels in commercial acacia honey for quality control: a systematic review. Discover Applied Sciences, 6(10), 515. ttps://doi.org/10.1007/s42452-024-06216-2

Altun, S. K., & Aydemir, M. E. (2021). Some Physical and chemical properties of raw flower-honey from Erzurum highland. Bee Studies, 13(2), 57-61. http://doi.org/10.51458/BSTD.2021.19

Anonim, (2024). Türkiye arıcılık haritası 2023 verileri ile güncellendi. Erişim Adresi: https://www.tarimorman.gov.tr/Haber/6280/Turkiye-Aricilik-Haritasi-2023-Verileri-Ile-Guncellendi. Erişim Tarihi: 14.01.2024.

Archilia, M. D., Neto, A. A. L., Marcucci, M. C., Alonso, R. C. B., de Camargo, T. C., Camargo, R. C., & Sawaya, A. C. H. F. (2023). Characterization of Brazilian monofloral and polyfloral honey by UHPLC-MS and classic physical-chemical analyses. Journal of Apicultural Research, 62(3), 578-589. https://doi.org/10.1080/00218839.2021.1886747

Baglio, E. (2018). Honey: processing techniques and treatments. Chemistry and Technology of Honey Production, 15-22.

Bakan A. (2009). Balın gizemi. Bilim ve Teknik Dergisi,10, 8-11.

Bengü, A. Ş., & Kutlu, M. A. (2018). Bingöl’de üretilen ballarda bazi kalite kriterlerinin belirlenmesi. Türk Doğa ve Fen Dergisi, 7(1), 7-10.

da Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry, 196, 309-323. http://dx.doi.org/10.1016/j.foodchem.2015.09.051

Doğanay A., & Girişgin A. O. (2017). Genel Arıcılık. Doğanay A., Aydın L. (Eds.), Bal Arısı (pp. 21-146), Dora Basım Yayım ve Dağıtım, Bursa.

European Food Safety Authority (EFSA) (2005). Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food (AFC) on a request from the commission related to flavouring group evaluation 13: furfuryl and furan derivatives with and without additional sidechain substituents and heteroatoms from chemical group 14. EFSA Journal, 215, 1-73.

Golmakani, M. T., Sasani, M., Sahraeian, S., & Khalesi, M. (2025). Evaluating the impact of thermal processing on physicochemical properties of monofloral and multifloral honey. Journal of Food Composition and Analysis, 137, 106940. https://doi.org/10.1016/j.jfca.2024.106940

Gültekin-Özgüven, M., Demircan, E., & Özçelik, B. (2020). Çeşitli yörelerimizde üretilen çiçek ballarının fizikokimyasal özelliklerinin belirlenmesi ve Türk Gıda Kodeksi’ne uygunluğunun değerlendirilmesi. Avrupa Bilim ve Teknoloji Dergisi, 20, 321-326. https://doi.org/10.31590/ejosat.758399

Hardt‐Stremayr, M., Mattioli, S., Greilberger, J., Stiegler, P., Matzi, V., Schmid, M. G., & Wintersteiger, R. (2013). Determination of metabolites of 5‐hydroxymethylfurfural in human urine after oral application. Journal of Separation Science, 36(4), 670-676. https://doi.org/10.1002/jssc.201200768

Hoxha, F., Kongoli, R., Malollari, I., Tosti, T., Milojkovic-Opsenica, D., & Tesic, Z. (2019). Analysis of some albanian market honey based on 5-hydroxymethylfurfural (HMF) content with its impact on human health. Journal of Environmental Protection and Ecology, 20(1), 496-504.

Kara, F., Kaya, C., Esin Yücel, E., Topuz, S., Bayram, M. (2022). Tokat yöresi ballarının bazı fizikokimyasal özelliklerinin belirlenmesi ve Türk gıda kodeksi Bal tebliğine uygunluğunun değerlendirilmesi. Uludağ Arıcılık Dergisi, 22(2), 148-165.

Khan, F. R., Abadin, Z. U., & Rauf, N. (2007). Honey: nutritional and medicinal value. International Journal of Clinical Practice, 61(10), 1705-1707. https://doi.org/10.1111/j.1742-1241.2007.01417.x

Kitts, D. D., Chen, X. M., & Jing, H. (2012). Demonstration of antioxidant and anti-inflammatory bioactivities from sugar–amino acid Maillard reaction products. Journal of Agricultural and Food Chemistry, 60(27), 6718-6727. https://doi.org/dx.doi.org/10.1021/jf2044636

Korkmaz, S. D. (2023). Determination of HMF Value and Diastase Activities in Strained Honeys Sold in Markets. Black Sea Journal of Engineering and Science, 6(4), 522-526. https://doi.org/10.34248/bsengineering.1349150

Korkmaz, S. D., & Küplülü, Ö. (2017). Effects of storage temperature on HMF and diastase activity of strained honeys. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 64(4), 281-287.

Lim, A. R., Sam, L. M., Gobilik, J., Ador, K., Choon, J. L. N., Majampan, J., & Benedick, S. (2022). Physicochemical properties of honey from contract beekeepers, street vendors and branded honey in Sabah, Malaysia. Tropical Life Sciences Research, 33(3), 61. https://doi.org/10.21315/tlsr2022.33.3.5

Machado, A. M., Tomás, A., Russo-Almeida, P., Duarte, A., Antunes, M., Vilas-Boas, M., ... & Figueiredo, A. C. (2022). Quality assessment of Portuguese monofloral honeys. Physicochemical parameters as tools in botanical source differentiation. Food Research International, 157, 111362. https://doi.org/10.1016/j.foodres.2022.111362

Nakib, R., Ghorab, A., Ouelhadj, A., Rodríguez-Flores, S., Escuredo, O., Bensouici, C., & Seijo-Coello, C. (2024). Chemometric evaluation of antioxidant activity and α-amylase inhibition of selected monofloral honeys from Algeria. Journal of Apicultural Research, 63(3), 570-580. https://doi.org/10.1080/00218839.2021.2005871

Pastoriza de la Cueva, S., Álvarez, J., Végvári, Á., Montilla‐Gómez, J., Cruz‐López, O., Delgado‐Andrade, C., & Rufián‐Henares, J. A. (2017). Relationship between HMF intake and SMF formation in vivo: An animal and human study. Molecular Nutrition & Food Research, 61(3), 1600773. https://doi.org/10.1002/mnfr.201600773

Pauliuc, D., Dranca, F., & Oroian, M. (2020). Antioxidant activity, total phenolic content, individual phenolics and physicochemical parameters suitability for Romanian honey authentication. Foods, 9(3), 306. https://doi.org/10.3390/foods9030306

Rosatella, A. A., Simeonov, S. P., Frade, R. F., & Afonso, C. A. (2011). 5-Hydroxymethylfurfural (HMF) as a building block platform: Biological properties, synthesis and synthetic applications. Green chemistry, 13(4), 754-793. https://doi.org/10.1039/c0gc00401d

Savaş, A., Binici, H. İ., Şat, İ. G., & Kiliç, M. (2024). Quality Assessment and Bioactive Component Analysis of Honey from Different Geographical Regions in Erzurum, Türkiye. Akademik Gıda, 22(3), 215-223. https://doi.org/10.24323/akademik-gida.1603870

Shapla, U. M., Solayman, M., Alam, N., Khalil, M. I., & Gan, S. H. (2018). 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health. Chemistry central journal, 12, 1-18. https://doi.org/10.1186/s13065-018-0408-3

Sönmez, B. (2004). Balın insan sağlığındaki yeri ve önemi. Uludağ Arıcılık Dergisi,127-130.

Subramanian, R., Umesh Hebbar, H., & Rastogi, N. K. (2007). Processing of honey: a review. International Journal of Food Properties, 10(1), 127-143. https://doi.org/10.1080/10942910600981708

Şahin, Ş., & Akdeniz, G. (2012). BAL: Besin ve şifa kaynağı olarak. Arıcılık Araştırma Dergisi, 7, 14-15.

Tigistu, T., Worku, Z., & Mohammed, A. (2021). Evaluation of the physicochemical properties of honey produced in Doyogena and Kachabira Districts of Kembata Tambaro zone, Southern Ethiopia. Heliyon, 7(4). https://doi.org/10.1016/j.heliyon.2021.e06803

Tkáč, M., Vorlová, L., Borkovcová, I., & Golian, J. (2022). Physicochemical and bioactive characterization of beekeeper and market honeys. Emirates Journal of Food and Agriculture, 34(4), 268-278. https://doi.org/10.9755/ejfa.2022.v34.i4.2851

Tornuk, F., Karaman, S., Ozturk, I., Toker, O. S., Tastemur, B., Sagdic, O., ... & Kayacier, A. (2013). Quality characterization of artisanal and retail Turkish blossom honeys: Determination of physicochemical, microbiological, bioactive properties and aroma profile. Industrial Crops and Products, 46, 124-131. http://dx.doi.org/10.1016/j.indcrop.2012.12.042

Toy, N. Ö., & Şahinler, N. (2022). Quality Parameters of Honey, an Important Bee Product. Turkish Journal of Agriculture - Food Science and Technology, 10(sp1), 2841-2847. https://doi.org/10.24925/turjaf.v10isp1.2841-2847.5859

Turhan, I., Tetik, N., Karhan, M., Gurel, F., & Tavukcuoglu, H. R. (2008). Quality of honeys influenced by thermal treatment. LWT-Food Science and Technology, 41(8), 1396-1399. https://doi.org/10.1016/j.lwt.2007.09.008

Türk Gıda Kodeksi Bal Tebliği, (Tebliğ No: 2020/7). Resmi Gazete Tarih: 22/04/2020, Sayı: 31107, Başbakanlık Basımevi, Ankara, Türkiye.

White J. J.W. (1980). Hydroxymethylfurfural content of honey as an indicator of its adulteration with invert sugars. Bee World, 61(1), 29-37.

Winkler, O. (1955). Beitrag zum Nachweis und zur Bestimmung von Oxymethylfurfurol in Honig und Kunsthonig. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 102(3), 161-167.

Yıldız, O., Şahin, H., Kara, M., Aliyazıcıoğlu, R., Tarhan, Ö., & Kolaylı, S. (2010). Maillard reaksiyonları ve reaksiyon ürünlerinin gıdalardaki önemi. Akademik Gıda, 8(6), 44-51.

Yilmaz, H., & Küfrevioğlu, Ö. İ. (2001). Composition of honeys collected from Eastern and South-Eastern Anatolia and effect of storage on hydroxymethylfurfural content and diastase activity. Turkish Journal of Agriculture and Forestry, 25(5), 347-349.

Zhao, L., Chen, J., Su, J., Li, L., Hu, S., Li, B., ... & Chen, T. (2013). In vitro antioxidant and antiproliferative activities of 5-hydroxymethylfurfural. Journal of Agricultural and Food Chemistry, 61(44), 10604-10611. https://dx.doi.org/10.1021/jf403098y

Downloads

Published

28.06.2025

How to Cite

Şahin, S. (2025). The Level of Hydroxymethylfurfural (HMF) in Strained Flower Honey Offered for Consumption in Sivas Province and Its Impact on Public Health. Turkish Journal of Agriculture - Food Science and Technology, 13(6), 1543–1549. https://doi.org/10.24925/turjaf.v13i6.1543-1549.7612

Issue

Section

Research Paper