Comparison of the Histological Structure of the Posterior Silk Gland in spinning Cocoons and Non-Spinning Silkworms (Bombyx mori L.)
DOI:
https://doi.org/10.24925/turjaf.v13i6.1571-1576.7702Keywords:
Silkworm, Silk Gland, Non-Spinning Syndrome, Pesticide , MetamorphosisAbstract
This study was conducted to compare the histological differences between the silk glands obtained from cocoon-spinning and non-cocoon-spinning silkworm larvae, and in this context, the changes that occur in the middle silk gland at the end of the larval period were examined using histological techniques. In the study, the cocoon spinning process started in the cocoon-spinning group as of the 7th day of the 5th instar and the cocoon spinning process was completed within the expected normal period. In the non-cocoon-spinning group, it was determined that the cocoon spinning process could not start within the normal period, and therefore the 5th instar period was extended until the 13th day. In the cocoon-spinning group, the tight connection of cells in the posterior silk gland, increased condensation in the nucleus, and the observation of autophagic vacuoles within the cells indicated that pupal metamorphosis continued healthily. On the other hand, in the non-cocoon-spinning group, the lack of nuclear condensation, the inability to distinguish autophagic vacuoles, and the absence of changes related to cell death indicated that the larval process continued. The regional observation of the non-spinning syndrome is thought to be due to pesticide residues in mulberry leaves. It is thought that the last instar in silkworm larvae is prolonged and the pupal metamorphosis process is prevented when mulberry leaves become contaminated with pesticides.
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