A Simple and Effective Method for Nucleic Acid and Pcr Product Purification: Magnetic Bead Technology
DOI:
https://doi.org/10.24925/turjaf.v13i9.2667-2670.7770Keywords:
Magnetic Bead Technology, Sanger sequencing, Nucleic acid PurificationAbstract
Alternative methods for nucleic acid purification have been developed in recent years. Alcohol precipitation, a low-cost alternative method for nucleic acid purification, requires a high degree of technical professionalism to ensure consistently good quality data. Proprietary kits utilizing the affinity chromatography methods give very good results but are quite costly. As an alternative to these methods, magnetic bead technology has become widely used for nucleic acid purification. There has been a significant increase in the use of magnetic beads due to their rapid, inexpensive, and easy applicability. Separation by magnetic beads is rapid and has minimal potential for user error. In this article, PCR products were purified by the magnetic bead method and sequenced by Sanger sequencing. The sequence data obtained were compared with sequence data obtained from the BigDye Terminator enzymatic purification method, and the advantages of using magnetic beads were evaluated.
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