Characterization of Morpho-Physiological Responses of Soybean Genotypes to Water Stress

Authors

DOI:

https://doi.org/10.24925/turjaf.v13i9.2686-2690.7804

Keywords:

Soybean genotypes, Drought stress, Chlorophyll, Nitrate reductase, Water stress tolerance, Crop resilience

Abstract

A study was conducted from February to May 2024 at the Bangladesh Institute of Nuclear Agriculture (BINA) pot yard in Mymensingh, Bangladesh, using a completely randomized design with four replications. The study aimed to assess the effects of different levels of water stress on seed yield and key physiological traits across five soybean genotypes. Five soybean genotypes (Binasoybean-7, SBM-15, SBM-22, SCM-5, and SCM-11) and three levels of water deficiency (control: no water deficiency, water deficiency 40%, and 60% field capacity) were imposed at the pre-flowering stage and continued until physiological maturity. Plant height, branch density, total dry mass, leaf nitrate reductase activity, pod and flower output, seed yield per pod, harvest index, and seed yield per plant were all significantly reduced across all five genotypes as the stress level increased due to dryness. The chlorophyll and total sugar levels in leaves across all five genotypes increased with the increasing rate of drought. Drought stress significantly decreased the rate of photosynthesis in leaves. Among the five studied genotypes, only the Binasoybean-7 variety shown superior performance under drought stress circumstances for morphological, physiological, and yield-contributing parameters. Therefore among the five genotypes Binasoybean-7 could be used as genetic material for developing more drought-tolerant soybean varieties.

References

Begg, J.E., and Turner, N.C. (1976). Crop water deficits. Adv. Agron. Academic press. 28: 191-217.

Benlioğlu, B., Demirel, F., Türkoğlu, A., Haliloğlu, K., Özaktan, H., Kujawa, S., Piekutowska, M., Wojciechowski, T., and Niedbała, G. (2024). Insights into Drought Tolerance of Tetraploid Wheat Genotypes in the Germination Stage Using Machine Learning Algorithms. Agriculture, 14(2), 206. https://doi.org/10.3390/agriculture14020206.

Chowdhury, J.A., Karim, M.A., Khaliq, Q.A., Ahmed, A.U., and Khan, M.S.A. (2016). Effect of drought stress on gas exchange characteristics of four soybean genotypes. Bangladesh J. Agril. Res. 41(2): 195-205.

Chowdhury, M. S. R., Sadia, J. F., Uddin, M. K., Rahaman, M., Shaha, P. K., and Saha, B. K. (2024). Soil Amendment with Biochar Reduces the Magnitude of Drought Stress in Soybean. Journal of the Bangladesh Agricultural University, 22(3), 317–325. https://doi.org/10.3329/jbau.v22i3.76404.

Dong, S., Jiang, Y., Dong, Y., Wang, L., Wang, W., Ma, Z., and Liu, L. (2019). A study on soybean responses to drought stress and rehydration. Saudi journal of biological sciences, 26(8), 2006-2017.

Farooq, M.A., Wahid, N., Kobayashi, D., Fujita, S.M., and Basra, A. (2009). Plant drought stress: effects, mechanisms and management. Agron. Sustain. Dev. 29: 185-212.

Hagemeyer, J. (1997). Salt. In: Plant Ecoplysiology. Prasad, M. N. V. (ed.). John Wiley and Sons. Inc. New York, Toronto Singapore. Pp. 174-205.

Hossain, M. S., Khan, M. A. R., Mahmud, A., Ghosh, U. K., Anik, T. R., Mayer, D., Das, A. K., and Mostofa, M. G. (2024). Differential Drought Responses of Soybean Genotypes in Relation to Photosynthesis and Growth-Yield Attributes. Plants, 13(19), 2765. https://doi.org/10.3390/plants13192765

Hussain, H.A., Men, S., Hussain, S., and Chen, Y. (2019). Interactive effects of drought and heat stresses on morphophysiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Scientific Reports, 9, 3890. https://doi.org/10.1038/s41598-019-40362-7.

Iqbal, N., Hussain, S., Raza, M.A., and Yang, C.Q. (2019). Drought tolerance of soybean (Glycine max L. Merr.) by improved photosynthetic characteristics and an efficient antioxidant enzyme activities under a split-root system. Frontiers in Physiology, 10, 786. https://doi.org/10.3389/fphys.2019.00786.

Lawlor, D.W., and Cornic, G. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in plants, Plant Cell Environ. 25: 275–294.

Makbul, S., Saruhan Güler, N., Durmus, N., and Güven, S. (2011). Changes in anatomical and physiological parameters of soybean under drought stress. Turk J. Bot. https://doi.org/10.3906/bot-1002-7.

Mangena, P. (2020b). Role of benzyladenine seed priming on growth and physiological and biochemical response of soybean plants growth under high salinity stress conditions. International Journal of Agronomy, ID 8847098.https://doi.org/10.1155/2020/8847098.

Mangena, P., and Mabulwana, P.T. (2022). Evaluations of Morpho-Physiological Variances in Soybean Varieties under Low Water Conditions. Journal of Experimental Biology and Agricultural Sciences, 10(1), 12–20. https://doi.org/10.18006/2022.10 (1).12.20.

Messina, M. (1997). Soy foods: Their role in disease prevention and treatment. In Liu, K. (ed). Soybeans: Chemistry, Technology and Utilization. Chapman and Hall, New York, Pp 442-466.

Mimi, A., Mannan, M.A., Khaliqand, Q.A., and Baset Mia, M.A. (2016). Yield response of soybean (Glycine max L.) genotypes to water deficit stress. Bangladesh Agron. J. 2016, 19(2): 51-60.

Monteoliva, M.I., Guzzo, M.C., and Posada, G. (2021). Breeding for Drought Tolerance by Monitoring Chlorophyll Content.

Sibole, J.V., Momtero, E., Cabot, C., Poschenrieder, C., and Barcelo, J. (1998). Role of sodium in the ABA-mediated long-term growth response of bean to salt stress. Physiol. Plant. 104, 299-305.

Singh, B.B., Hartmann, P., Fatokun, C., Tamo, M., Tarawali, S., and Ortiz, R. (2003). Recent progress on cowpea improvement. Chron. Hortic. 43: 8-12.

Steward, G.R., and Orebamjo, T.O. (1979). Some unusual characteristics of nitrate reduction in Erythrana senegalensis. New Phytol., 83: 311-319.

Tardieu, F., and Davies, W.J. (1996). Root-shoot communication and whole plant regulation of water flux: water deficit in plants. Bios. Sci. publ.. 147-162.

Wu, Z., and Zhang, Y. (2019). Effects of exogenous auxin on physiological and biochemical characteristics of soybean under PEG simulated drought stress. Hubei Agric. Sci. 58(6) 16-19, 23.

Yaşar, M. (2023). Sensitivity of different flax (Linum usitatissimum L.) genotypes to salinity determined by GE biplot. Saudi journal of biological sciences, 30(4), 103592. https://doi.org/10.1016/j.sjbs.2023.103592.

Yoshida, S.D.A., Forno, J.A., Cock, and Gomes, K.A. (1976). Laboratory manual for physiological studies of rice. 3rd ed., IRRI, Los Banos, Philippines. pp. 63-64.

Zhang, J., Liu, J., Yang, C., Du, S., and Yang, W. (2016). Photosynthetic performance of soybean plants to water deficit under high and low light intensity. S. Afr. J. Bot. 105, 279–287.

Zhu, J.K. (2002). Salt and drought stress signal transduction in plants. Annual Review of Plant Biology. 53: 247-273

Downloads

Published

27.09.2025

Issue

Section

Research Paper