Effects of Azolla Incorporation Timing on Growth and Yield Performance of Spring Rice (Oryza sativa L.)
DOI:
https://doi.org/10.24925/turjaf.v13is1.2420-2428.7990Keywords:
Azolla, Bio-fertilizer, Incorporation, Inoculation, Nitrogen, Spring riceAbstract
Spring rice in Nepal often faces nitrogen shortages during peak crop demand, limiting yield potential. Azolla, a nitrogen-fixing aquatic fern, offers an eco-friendly alternative, but its effectiveness depends on inoculation and incorporation timing. A field experiment was conducted in Sapahi, Bara, Nepal (March–June, 2023) using a Randomized Complete Block Design with seven treatments and three replications. Plots measured 2 × 3 m (6 m²). Treatments comprised Azolla inoculated at 25, 35, 45, 55, and 65 days after transplanting (DAT) with recommended P & K, a recommended NPK dose (RDF), and a control. Data on growth, yield, and yield components were recorded and analyzed using ANOVA in RStudio. Treatment means were separated using Duncan’s Multiple Range Test (DMRT) at the 5% significance level. RDF recorded the highest grain yield (4.25 t/ha) and effective tillers (302.67 m-2), significantly higher than all other treatments (p < 0.001; LSD = 0.050). The best Azolla treatment, 25 DAT + P & K (4.02 t/ha), outperformed later Azolla applications and the control (1.87 t/ha). T1 also achieved more effective tillers (259.67m-²), longer panicles (23.72 cm), and lower sterility (25.44%) than other Azolla timings (p < 0.001). Azolla application at 25 DAT with P & K improves yield and reduces reliance on synthetic nitrogen fertilizers, making it a sustainable option for spring rice production in Nepal.
References
Azizi, M., & Safriani, S. R. (2024). Integration of Rice, Ducks and Azolla on Growth and Yield of Rice Plants (Oryza Sativa L). SEAS (Sustainable Environment Agricultural Science), 8(2), 126–132. https://doi.org/10.22225/seas.8.2.10779.126-132
Bazihizina, N., Paleni, C., Caparrotta, S., Macchiavelli, T., Guardigli, G., Colzi, I., Petrillo, M., Gonnelli, C., Saccomanno, A., Gregis, V., Mancuso, S., Comparini, D., Kater, M. M., & Pandolfi, C. (2025). Azolla mediated alterations in grain yield and quality in Rice. Physiologia Plantarum, 177(2), e70158. https://doi.org/10.1111/ppl.70158
Bhattarai, K., Pandey, K. R., Marahatta, S., Dhakal, M., Bhusal, N. R., & Thapa, R. (2024). Precision Nitrogen Management in Spring Rice (Oryza sativa L.) using Decision Support Tools in Chitwan, Nepal. Turkish Journal of Agriculture - Food Science and Technology, 12(6), Article 6. https://doi.org/10.24925/turjaf.v12i6.955-965.6731
Bhuvaneshwari, K., & Singh, P. K. (2015). Response of nitrogen-fixing water fern Azolla biofertilization to rice crop. 3 Biotech, 5(4), 523–529. https://doi.org/10.1007/s13205-014-0251-8
Chen, X., Cui, Z., Fan, M., Vitousek, P., Zhao, M., Ma, W., Wang, Z., Zhang, W., Yan, X., Yang, J., Deng, X., Gao, Q., Zhang, Q., Guo, S., Ren, J., Li, S., Ye, Y., Wang, Z., Huang, J., … Zhang, F. (2014). Producing more grain with lower environmental costs. Nature, 514(7523), 486–489. https://doi.org/10.1038/nature13609
Consorti, E., Costarelli, A., Cannavò, S., Cerri, M., Valeri, M. C., Reale, L., Saccomano, A., Paleni, C., Gregis, V., Kater, M. M., Brilli, F., Paolocci, F., & Ghirardo, A. (2024). Co-cultivation with Azolla affects the metabolome of whole rice plant beyond canonical inorganic nitrogen fertilization. Plant Biology. https://doi.org/10.1101/2024.10.02.615589
Cui, Z., Zhang, H., Chen, X., Zhang, C., Ma, W., Huang, C., Zhang, W., Mi, G., Miao, Y., Li, X., Gao, Q., Yang, J., Wang, Z., Ye, Y., Guo, S., Lu, J., Huang, J., Lv, S., Sun, Y., … Dou, Z. (2018). Pursuing sustainable productivity with millions of smallholder farmers. Nature, 555(7696), 363–366. https://doi.org/10.1038/nature25785
Devkota, P., Dhakal, S., Shrestha, S., & Shrestha, U. B. (2023). Land use land cover changes in the major cities of Nepal from 1990 to 2020. Environmental and Sustainability Indicators, 17, 100227. https://doi.org/10.1016/j.indic.2023.100227
Ghimire, A., Nainawasti, A., Shah, T., & Dhakal, S. (2021). Effect of Different Bio Fertilizers on Yield Of Spring Rice (Oryza Sativa L.) CV. Hardinath-1 in Rajapur Municipality, Bardiya. SAARC Journal of Agriculture, 19(1), 57–69. https://doi.org/10.3329/sja.v19i1.54778
Hassan, M. U., Aamer, M., Mahmood, A., Awan, M. I., Barbanti, L., Seleiman, M. F., Bakhsh, G., Alkharabsheh, H. M., Babur, E., Shao, J., Rasheed, A., & Huang, G. (2022). Management Strategies to Mitigate N2O Emissions in Agriculture. Life, 12(3), Article 3. https://doi.org/10.3390/life12030439
Kandel, S., Malla, R., Adhikary, B. H., & Vista, S. P. (2020). EFFECT OF AZOLLA APPLICATION ON RICE PRODUCTION AT MID-HILLS CONDITION OF NEPAL. Tropical Agroecosystems, 1(2), 103–106. https://doi.org/10.26480/taec.02.2020.103.106
Kumar, B., & Shahi, D. K. (2016). Effect of Azolla as Green Manure on Soil Properties and Grain Yield of Rice in Acid Soil of Jharkhand. Annals of Plant and Soil Research, 18(3), 214–218.
Kumar, S., Diksha, Sindhu, S. S., & Kumar, R. (2022). Biofertilizers: An ecofriendly technology for nutrient recycling and environmental sustainability. Current Research in Microbial Sciences, 3, 100094. https://doi.org/10.1016/j.crmicr.2021.100094
Lellinger, D. B. (1983). Azolla as a Green Manure: Use and Management in Crop Production. American Fern Journal, 73(3), 96–96. JSTOR. https://doi.org/10.2307/1546860
M, I. N., I, J. D., M D, S., K, G., Chandramauli, A., & Singh, R. (2024). Nano technology - Infused Azolla Manure: Improving Rice Stem Thickness in Sustainable Agriculture. E3S Web of Conferences, 588, 01012. https://doi.org/10.1051/e3sconf/202458801012
M. Tahat, M., M. Alananbeh, K., A. Othman, Y., & I. Leskovar, D. (2020). Soil Health and Sustainable Agriculture. Sustainability, 12(12), 4859. https://doi.org/10.3390/su12124859
Marahatta, S. (2022). Effects of coated and briquette urea on yield and nitrogen use efficiency of rice at Rampur, Chitwan, Nepal. Journal of Agriculture and Forestry University, 219–227. https://doi.org/10.3126/jafu.v5i1.48468
Marti-Jerez, K., Català-Forner, M., Tomàs, N., Murillo, G., Ortiz, C., Sánchez-Torres, M. J., Vitali, A., & Lopes, M. S. (2023). Agronomic performance and remote sensing assessment of organic and mineral fertilization in rice fields. Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1230012
Marzouk, S. H., Semoka, J. M., Amuri, N. A., & Tindwa, H. J. (2024). Rice straw incorporation and Azolla application improves agronomic nitrogen-use-efficiency and rice grain yields in paddy fields. Frontiers in Soil Science, 4, 1378065. https://doi.org/10.3389/fsoil.2024.1378065
Marzouk, S. H., Tindwa, H. J., Amuri, N. A., & Semoka, J. M. (2023). An overview of underutilized benefits derived from Azolla as a promising biofertilizer in lowland rice production. Heliyon, 9(1), e13040. https://doi.org/10.1016/j.heliyon.2023.e13040
McLaughlin, A., & Mineau, P. (1995). The impact of agricultural practices on biodiversity. Agriculture, Ecosystems & Environment, 55(3), 201–212. https://doi.org/10.1016/0167-8809(95)00609-V
MoALD. (2024a). Agriculture and Livestock Diary 2082 (p. 380) [Annual Report]. https://aitc.gov.np/uploads/documents/agriculture-diary-2082-webpdf-2343-724-1751537264.pdf
MoALD. (2024b). Agriculture Diary. https://aitc.gov.np/uploads/documents/agriculture-diary-2081-file-2081-03-2pdf-6568-329-1719146649.pdf
MoALD. (2024c). STATISTICAL INFORMATION ON NEPALESE AGRICULTURE 2080/81(2023/24) (p. 224) [Annual Report]. Government of Nepal Ministry of Agriculture and Livestock Development Planning and Development Cooperation Coordination Division Statistics and Analysis Section Singhadurbar, Kathmandu. https://giwmscdnone.gov.np/media/pdf_upload/Statistical%20Information%20on%20Nepalese%20Agriculture%2C%202080_81_a7s8aj4.pdf
Ocloo, X. S., Mbaye, M., Counta, A. S. D. T. M., Vazquez-Prokopec, G. M., Jouanard, N., Seck, M., Sow, S. L. T., & Civitello, D. J. (2025). Urea-ka: Replacing inorganic nitrogen fertilizer with Azolla pinnata maintains rice yields with reduced input costs in the Senegal River Valley. International Journal of Agricultural Sustainability, 23(1), 2469325. https://doi.org/10.1080/14735903.2025.2469325
Oyange, W., G., N., J., I., & Nthakanio, P. (2020). Effect of time of Azolla incorporation and inorganic fertilizer application on growth and yield of Basmati rice. African Journal of Agricultural Research, 15, 464–472.
Pahalvi, H. N., Rafiya, L., Rashid, S., Nisar, B., & Kamili, A. N. (2021). Chemical Fertilizers and Their Impact on Soil Health. In G. H. Dar, R. A. Bhat, M. A. Mehmood, & K. R. Hakeem (Eds.), Microbiota and Biofertilizers, Vol 2: Ecofriendly Tools for Reclamation of Degraded Soil Environs (pp. 1–20). Springer International Publishing. https://doi.org/10.1007/978-3-030-61010-4_1
Palikhe, A., Gupta, S., Maharjan, N. K., Adhikari, M., Kumar, A., Minot, N., Koirala, P., & Krupnik, T. J. (2024). Situation Report on Nepal’s Agrifood Systems (June 2024 Bulletin Number 19). The Cereal Systems Initiative for South Asia (CSISA) and Transforming Agrifood Systems in South Asia (TAFSSA).
Panta, H. K. (2018). Supply Chain of Subsidized Chemical Fertilizers in Nepal. Journal of the Institute of Agriculture and Animal Science, 35(1), Article 1. https://doi.org/10.3126/jiaas.v35i1.22509
Regmi, N. R., Bhandari, M. K., Ghimire, P., & Panthi, B. (2023). STATUS AND PROSPECTS OF SPRING RICE IN NEPAL: A REVIEW. I TECH MAG, 5, 01–05. https://doi.org/10.26480/itechmag.05.2023.01.05
Rohayani, M., Widodo, W., Masdar, M., & Gusmara, H. (2024). The Effect of Foliar Application of Azolla Liquid Organic Fertilizer on Growth and Yield of Rice in Swampy Soil. TERRA : Journal of Land Restoration, 7(1), 14–22. https://doi.org/10.31186/terra.7.1.14-22
Sarkar, S., Ray, K., Garai, S., Banerjee, H., Haldar, K., & Nayak, J. (2023). Modelling nitrogen management in hybrid rice for coastal ecosystem of West Bengal, India. PeerJ, 11, e14903. https://doi.org/10.7717/peerj.14903
Shrestha, J., Subedi, S., Singh Kushwaha, U. K., & Maharjan, B. (2021). Evaluation of growth and yield traits in rice genotypes using multivariate analysis. Heliyon, 7(9), e07940. https://doi.org/10.1016/j.heliyon.2021.e07940
Tuladhar, J. K. (2004). The Effect of Azolla on Nitrogen Use Efficiency in Rice-wheat Rotations of Nepal (Vol. 13). Cuvillier Verlag. https://books.google.com.np/books?id=4jiBxwQZRtoC
Watanabe, I., Bai Ke-Zhi, B. K.-Z., Berja, N., Espinas, C., Ito, O., & Subudhi, B. (1981). The Azolla-Anabaena complex and its use in rice culture. IRRI Research Paper Series, No.69, 11pp. CABI Databases.
Xu, H., Zhu, B., Liu, J., Li, D., Yang, Y., Zhang, K., Jiang, Y., Hu, Y., & Zeng, Z. (2017). Azolla planting reduces methane emission and nitrogen fertilizer application in double rice cropping system in southern China. Agronomy for Sustainable Development, 37(4), 29. https://doi.org/10.1007/s13593-017-0440-z
Yin, M., Tong, X., Yang, J., Cheng, Y., Zhou, P., Li, G., Wang, Y., & Ying, J. (2024). Dissecting the Genetic Basis of Yield Traits and Validation of a Novel Quantitative Trait Locus for Grain Width and Weight in Rice. Plants, 13(6), Article 6. https://doi.org/10.3390/plants13060770
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