Comparative Analysis of Green Extraction Techniques for Functional Ingredients from Cereal Wastes: A Sustainability Perspective

Authors

DOI:

https://doi.org/10.24925/turjaf.v13i12.4286-4307.8024

Keywords:

Cereal by-products, Functional Food, green extraction, Circular economy, waste valorization, sustainable production

Abstract

Cereal processing produces a significant amount of lignocellulosic waste as by-products such as bran, husks, and germ fractions that are commonly discarded or consumed for low value but contains a number of bioactive compounds with antioxidant, antimicrobial and health promoting activities. Under these circumstances, cereal wastes, due to their natural functionality and availability, also become a promising source towards promoting sustainable innovations that are particularly efficacious to provide economic incentives in agri-food systems as well as in food, nutraceutical, and cosmetic industries. This review evaluates an eco-friendly extraction for the recovery of bioactive compounds in cereal-processing by-products and critically evaluates its performance in terms of sustainability. Various green extraction techniques were compared in terms of yield, selectivity, extraction time and solvent volume usage, energy input and environmental impact. These involve novel physical and biochemical methods that work under less severe conditions compared to traditional solvent-based methods. Results indicate that some methods allow fast and low-energy-consuming extraction of thermally labile constituents with little degradation, while some are especially selective and good for recovery of individual substances. Yet, the practical complexity, scalable constituting reactions and the stability of the compounds are at trade-offs. The study demonstrates that there is no universal extraction methodology and that the selection depends on the final product requirements and potential resources. It highlights the technical efficiency and environmental benefits and the cereal waste can be turned in high added-value ingredient by circular bioeconomy approach. In conclusion, it believes the green extraction is an advantageous method to improve the efficiency resource as well as a special way to decrease waste in the products and enhance sustainable production.

References

Akin, M., Jukic, M., Lukinac, J., & Yilmaz, B. (2025). Valorization and functionalization of cereal-based industry by-products for nutraceuticals. In Valorization of Food Processing By-products (pp. 117–140). Wiley. https://doi.org/10.1002/9781394174867.ch6

Alavi, A., Mokarram, R. R., & Mortazavi, S. A. (2021). Microwave-assisted extraction from mixed cereals: Compliance with green extraction criteria. Innovative Food Science & Emerging Technologies, 72, 102757. https://doi.org/10.1016/j.ifset.2021.102757

Alonso, E. (2018). The role of supercritical fluids in the fractionation pretreatments of a wheat bran-based biorefinery. The Journal of Supercritical Fluids, 133, 728–735. https://doi.org/10.1016/j.supflu.2017.12.019

Amoriello, T., Mellara, F., Ciorba, R., Ceccarelli, D., Amoriello, M., Taddei, F., & Ciccoritti, R. (2025). Phenols extraction from sorghum byproducts: Upcycling strategies and food applications. Antioxidants, 14(6), 668. https://doi.org/10.3390/antiox14060668

Arumuganainar, D. G., Ramesh, D. L., Pandurangan, P., Sunkar, S., Abraham, S., Samrot, A. V., et al. (2025). Sustainable pectin extraction: Navigating industrial challenges and opportunities with fruit by-products – A review. Process Biochemistry, 154, 234–245. https://doi.org/10.1016/j.procbio.2025.04.025

Arzami, A. N., Ho, T. M., & Mikkonen, K. S. (2022). Valorization of cereal by-product hemicelluloses: Fractionation and purity considerations. Food Research International, 151, 110872. https://doi.org/10.1016/j.foodres.2021.110872

Ballesteros-Gómez, A., & Torres-Valenzuela, L. S. (2020). DES extraction of polyphenols from barley. Springer Protocols. https://doi.org/10.1007/S12393-019-09206-Y

Basile, G., De Luca, L., & Sorrentino, G. (2023). Green technologies for extracting plant waste functional ingredients and new food formulation. Journal of Food Science. https://doi.org/10.1111/1750-3841.17487

Basile, G., De Luca, L., Sorrentino, G., Cavella, S., & Romano, R. (2024). Green technologies for extracting plant waste functional ingredients and new food formulation: A review. Journal of Food Science, 89(5), 1335–1354. https://doi.org/10.1111/1750-3841.17487

Bekavac, N., Krog, K., Stanić, A., Šamec, D., & Šalić, A. (2025). Valorization of food waste: Extracting bioactive compounds for sustainable health and environmental solutions. Antioxidants, 14(6), 714. https://www.mdpi.com/2076-3921/14/6/714

Beltrán, J. L., & Cortina, J. L. (2024). Enhanced flavonol recovery via DES. LWT - Food Science and Technology. https://doi.org/10.1016/j.lwt.2024.114121

Bezerra, F. S., & Koblitz, M. G. B. (2024). Extraction of phenolic compounds from agro-industrial by-products using natural deep eutectic solvents. Separations, 12(6), 150. https://doi.org/10.3390/separations12060150

Bhat, A. R., Maled, S. B., Hegde, S., & Sivamani, Y. (2024). Enzyme-assisted extraction using grains and cereals. In Bioactive Extraction and Nutraceutical Industry. https://doi.org/10.1007/978-1-0716-3601-3_8

Bhatia, L., Kaladhar, D. S., & Jha, H. (2024). Food wastes phenolic compounds: greener extraction technologies and bioeconomy integration. Energy, Ecology and Environment. https://doi.org/10.1007/s40974-024-00321-z

Bhatia, L., Kaladhar, D. S., Sarkar, T., & Jha, H. (2024). Food wastes phenolic compounds (PCs): Overview of contemporary greener extraction technologies, industrial potential, and its integration into circular bioeconomy. Energy, Ecology and Environment. https://doi.org/10.1007/s40974-024-00321-z

Bodoira, R. M., Cardona Jimenez, M. E., & Gabilondo, J. (2024). Extraction of bioactive compounds from pecan nutshell: An added-value and low-cost alternative for an industrial waste. CONICET Digital. https://notablesdelaciencia.conicet.gov.ar/handle/11336/261124

Bragagnolo, F. S., Strieder, M. M., & Pizani, R. S. (2024). Revisiting natural deep eutectic solvents (NADES) as extraction media and ready-to-use purposes. TrAC Trends in Analytical Chemistry, 117638. https://doi.org/10.1016/j.trac.2024.117638

Burlini, I. (2020). Cereal by-products as source of bioactive molecules for cosmetic applications: A sustainable approach [Doctoral dissertation, University of Ferrara]. https://sfera.unife.it/handle/11392/2478831

Buvaneshwaran, M., & Radhakrishnan, M. (2023). Influence of ultrasound-assisted extraction techniques on the valorization of agro-based industrial organic waste: A review. Journal of Food Process Engineering. https://doi.org/10.1111/jfpe.14012

Cannavacciuolo, C., Pagliari, S., Frigerio, J., & Giustra, C. M. (2022). Natural deep eutectic solvents (NADESs) combined with sustainable extraction techniques: A review of the green chemistry approach in food analysis. Foods, 12(1), 56. https://doi.org/10.3390/foods12010056

Carpentieri, S., Soltanipour, F., Ferrari, G., Pataro, G., & Donsì, F. (2021). Emerging green techniques for the extraction of antioxidants from agri-food by-products. Antioxidants, 10(9), 1417. https://doi.org/10.3390/antiox10091417

Castro-Muñoz, R., & Yáñez-Fernández, J. (2023). Green ultrasound methods for bioactive extraction from grain processing residues. Current Opinion in Food Science, 51, 100971. https://doi.org/10.1016/j.cofs.2023.100971

Celano, R., Campone, L., Piccinelli, A. L., & Pagano, I. (2021). Green non-conventional techniques for the extraction of polyphenols from agricultural food by-products: A review. Journal of Chromatography A, 1656, 462500. https://doi.org/10.1016/j.chroma.2021.462500

Celano, R., Pagliari, S., & Cannavacciuolo, C. (2024). Critical analysis of green extraction techniques used for botanicals. TrAC Trends in Analytical Chemistry, 163, 117999. https://doi.org/10.1016/j.trac.2024.117999

Chanioti, S., & Siamandoura, P. (2023). Green technologies in food processing. In Green Chemistry in Food. https://doi.org/10.1201/9780429289538-8

Chemat, F., Vian, M. A., & Cravotto, G. (2012). Green extraction of natural products: Concept and principles. International Journal of Molecular Sciences, 13(7), 8615–8627. https://doi.org/10.3390/ijms13078615

Chemat, F., Rombaut, N., Sicaire, A.-G., Meullemiestre, A., Fabiano-Tixier, A.-S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035

Chen, W., Zheng, X., & Li, Y. (2023). High radical scavenging capacity of wheat bran extracts obtained by MAE. Antioxidants, 12(4), 785. https://doi.org/10.3390/antiox12040785

Cherif, M. M., Grigorakis, S., & Halahlah, A. (2020). High-efficiency extraction of phenolics from wheat waste biomass using DES, ultrasound and thermal pretreatment. Environmental Science and Pollution Research. https://doi.org/10.1007/s40710-020-00449-0

Chiș, A., & Fărcaș, A. C. (2025). Enzyme-assisted fermentation of bakery waste for antioxidant recovery. [Under review / DOI pending]

Chiș, M. S., & Fărcaș, A. C. (2024). Cereal by-products valorization in bakery, pastry, and gastronomy products manufacturing. IntechOpen. https://www.intechopen.com/online-first/1178073

Coca, M., & Campillo, V. (2021). MAE modeling of NADES from orange peel. Journal of Chemical Technology and Biotechnology. https://doi.org/10.1002/jctb.6565

Coelho, J. A., Pinto, T. I., Pires, B. I., Neng, N. R., & Nogueira, J. M. F. (2021). Supercritical CO₂ extraction and fatty acid composition of rice bran oil from Portuguese varieties. Separations, 8(8), 115. https://doi.org/10.3390/separations8080115

Colombo, R., Moretto, G., Barberis, M., Frosi, I., & Papetti, A. (2023). Rice byproduct compounds: From green extraction to antioxidant properties. Antioxidants, 13(1), 35. https://doi.org/10.3390/antiox13010035

Cortina, J. L., Beltrán, J. L., & Ramos-Calero, V. (2024). Polymeric sorbents with NADES for polyphenol recovery. LWT - Food Science and Technology. https://doi.org/10.1016/j.lwt.2024.114121

Custodio-Mendoza, J. A., Rangel Silva, A., Pokorski, P., Aktaş, H., & Kurek, M. A. (2025). Green sample preparation for anthocyanin extraction from purple corn: Analytical evaluation of pressurized liquid and ultrasound-assisted extraction using sustainable solvents. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-025-05951-8

da Silva, L. H. M., Rodrigues, A. M. C., & Pires, I. V. (2024). NADES for anthocyanin extraction: Economics and challenges. Comprehensive Reviews in Food Science and Food Safety, 23(4), e70057. https://doi.org/10.1111/1541-4337.70057

Danciu, C. A., Tulbure, A., Stanciu, M. A., & Antonie, I. (2023). Overview of the sustainable valorization of using waste and by-products in grain processing. Foods, 12(20), 3770. https://www.mdpi.com/2304-8158/12/20/3770

Dapčević-Hadnađev, T., Hadnađev, M., & Pojić, M. (2018). The healthy components of cereal by-products and their functional properties. In Reutilization of Cereal Processing By-products (pp. 25–47). Elsevier. https://doi.org/10.1016/B978-0-08-102162-0.00002-2

Darvishzadeh, P., & Orsat, V. (2022). Microwave-assisted extraction of antioxidant compounds from Russian olive leaves and flowers: Optimization, HPLC characterization and comparison with other methods. Journal of Applied Research on Medicinal and Aromatic Plants, 27, 100368. https://doi.org/10.1016/j.jarmap.2021.100368

Demirbaş, A., & Duran, Y. (2023). Extraction of ferulic acid from maize cobs using MAE: Food-grade application. Industrial Crops and Products, 197, 116504. https://doi.org/10.1016/j.indcrop.2022.116504

Djaoudene, O., Bachir-Bey, M., Schisano, C., & Djebari, S. (2024). Sustainable extraction of phytochemicals using ultrasound-assisted deep eutectic solvent. Antioxidants, 13(2), 181. https://doi.org/10.3390/antiox13020181

Domínguez-Rodríguez, G., García, M. C., & Marina, M. L. (2021). Combined pressurized liquid and enzymatic-assisted extraction for non-extractable polyphenols from sweet cherry by-products. Nutrients, 13(9), 3242. https://doi.org/10.3390/nu13093242

Domínguez-Rodríguez, G., García, M. C., & Marina, M. L. (2021). Pressurized liquid extraction combined with enzymatic-assisted extraction to obtain bioactive non-extractable polyphenols from sweet cherry (Prunus avium L.) pomace. Nutrients, 13(9), 3242. https://doi.org/10.3390/nu13093242

Dukić, J. (2025). Utilization of by-products and waste from sugar beet (Beta vulgaris L.) processing by non-thermal extraction techniques [Doctoral dissertation, University of Zagreb]. https://urn.nsk.hr/urn:nbn:hr:159:748893

Fărcaș, A. C., Socaci, S. A., Nemeș, S. A., & Salanță, L. C. (2022). Cereal waste valorization through conventional and current extraction techniques. Foods, 11(16), 2454. https://doi.org/10.3390/foods11162454

Fărcaș, A. C., Socaci, S. A., Novotni, D., & García-Vaquero, M. (2023). Innovative technologies to extract high-value compounds. In Sourdough Innovations (pp. 87–110). Taylor & Francis. https://doi.org/10.1201/9781003141143-5

Fărcaș, A., Drețcanu, G., Pop, T. D., Enaru, B., & Socaci, S. (2021). Cereal processing by-products as rich sources of phenolic compounds. Nutrients, 13(11), 3934. https://doi.org/10.3390/nu13113934

Fernandes, F., Delerue-Matos, C., & Grosso, C. (2024). Unveiling the potential of agrifood by-products: A comprehensive review of phytochemicals, bioactivities and industrial applications. Waste and Biomass Valorization. https://doi.org/10.1007/s12649-024-02622-0

Ferraz, R., Grosso, C., & Delerue-Matos, C. (2022). A critical comparison of advanced extraction techniques for bioactive compounds. Marine Drugs, 20(11), 677. https://doi.org/10.3390/md20110677

Ferreira, S. S., Silva, A. M., & Nunes, F. M. (2020). Microwave-assisted extraction of phenolic acids from barley husk: Optimization and characterization. Food Chemistry, 325, 126926. https://doi.org/10.1016/j.foodchem.2020.126926

Fraguela-Meissimilly, H., Bastías-Monte, J. M., & Vergara, C. (2023). Pressurized liquids and supercritical fluids for bioactive extraction. Molecules, 28(11), 4421. https://doi.org/10.3390/molecules28114421

Franca-Oliveira, G., Fornari, T., & Hernández-Ledesma, B. (2021). A review on the extraction and processing of natural source-derived proteins through eco-innovative approaches. Processes, 9(9), 1626. https://doi.org/10.3390/pr9091626

Galanakis, C. M. (2022). Sustainable applications for the valorization of cereal processing by-products. Foods, 11(2), 241. https://doi.org/10.3390/foods11020241

Gallo, V., De Paola, L., & Mazzeo, L. (2023). DES design for cereal pericarp valorization. Separation and Purification Technology. https://doi.org/10.1016/j.seppur.2023.124374

Gauffre, F., Percevault, L., & Lagrost, C. (2021). Extraction of plant polyphenols with DES. In Deep Eutectic Solvents and Their Applications (pp. 111–131). Springer. https://doi.org/10.1007/978-3-030-53069-3_7

Franca-Oliveira, G., Fornari, T., & Hernández-Ledesma, B. (2021). A review on the extraction and processing of natural source-derived proteins through eco-innovative approaches. Processes, 9(9), 1626. https://doi.org/10.3390/pr9091626

Galanakis, C. M. (2022). Sustainable applications for the valorization of cereal processing by-products. Foods, 11(2), 241. https://doi.org/10.3390/foods11020241

Gallo, V., De Paola, L., & Mazzeo, L. (2023). DES design for cereal pericarp valorization. Separation and Purification Technology. https://doi.org/10.1016/j.seppur.2023.124374

Gauffre, F., Percevault, L., & Lagrost, C. (2021). Extraction of plant polyphenols with DES. In Deep Eutectic Solvents and Their Applications (pp. 111–131). Springer. https://doi.org/10.1007/978-3-030-53069-3_7

Ghosh, P., & Vasanth, V. (2021). Extraction of polyphenols from rye bran using MAE: Efficiency and sustainability. Journal of Food Process Engineering, 44(5), e13667. https://doi.org/10.1111/jfpe.13667

González-García, E., Marina, M. L., & García, M. C. (2021). Pressurized liquids for extraction of proteins and bioactives from brewer’s spent grain. Food Chemistry, 340, 128169. https://doi.org/10.1016/j.foodchem.2020.128169

Guler, B. A., & Tepe, U. (2024). Sustainable point of view: Life cycle analysis for green extraction technologies. ChemBioEng Reviews. https://doi.org/10.1002/cben.202300056

Guo, H., Wu, H., Sajid, A., & Li, Z. (2022). Whole grain cereals: The potential roles of functional components in human health. Critical Reviews in Food Science and Nutrition. https://doi.org/10.1080/10408398.2021.1928596

Guo, Z., Deng, X., Ping, C., Li, X., Li, D., Wu, X., & Xiao, X. (2025). Quinoa: Nutritional and phytochemical value, beneficial effects, and future applications. Applied Food Research. https://www.sciencedirect.com/science/article/pii/S2772502225000769

Hanafi, M. A., Anwar, F., & Saari, N. (2024). Valorization of cereal waste protein via NADES. Food Frontiers. https://doi.org/10.1002/fft2.389

Hashemi, B., Shiri, F., Švec, F., & Nováková, L. (2022). Green solvents and approaches recently applied for extraction of natural bioactive compounds. TrAC Trends in Analytical Chemistry, 152, 116618. https://doi.org/10.1016/j.trac.2022.116618

Hernández-Corroto, E., Marina, M. L., & García, M. C. (2024). Simultaneous and sequential combination of techniques for the sustainable and extensive extraction of proteins and polyphenols from malt rootlets. Advances in Sample Preparation, 6, 100113.

https://doi.org/10.1016/j.sampre.2024.100113

Herrero, M., Cifuentes, A., & Ibáñez, E. (2006). Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae. Food Chemistry, 98(1), 136–148.

https://doi.org/10.1016/j.foodchem.2005.05.058

Jain, I., Kaur, R., Kumar, A., & Paul, M. (2024). Emerging protein sources and novel extraction techniques. International Journal of Food Science & Technology. https://doi.org/10.1111/ijfs.17466

Jukic, M., Akin, M., Lukinac, J., & Yilmaz, B. (2025). Functional lipids and phytochemicals from cereal germ. In Functional Foods Processing and Health Benefits (pp. 145–168). Wiley. https://doi.org/10.1002/9781394174867.ch6

Jyoti, T. P., & Shrivastav, G. (2024). Eco-friendly extraction: Innovations and comparison with traditional methods. Separation & Purification Reviews. https://doi.org/10.1080/15422119.2024.2381605

Kaladhar, D. S., Sarkar, T., & Bhatia, L. (2024). Circular bioeconomy via DES extraction of sorghum bran. Energy, Ecology and Environment. https://doi.org/10.1007/s40974-024-00321-z

Khalid, W., Benmebarek, I. E., Zargarchi, S., Kumar, P., Javed, M., Moreno, A., Sharma, A., Nayik, G. A., & Esatbeyoglu, T. (2025a). Optimization of the effect of cold plasma treatment on UAE-NADES green extraction of chickpea roots (Cicer arietinum) bioactive compounds. Ultrasonics Sonochemistry, 114, 107276. https://doi.org/10.1016/j.ultsonch.2025.107276

Khalid, W., Koraqi, H., Benmebarek, I. E., Moreno, A., Alsulami, T., Mugabi, R., & Nayik, G. A. (2025b). Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology. Ultrasonics Sonochemistry, 112, 107186. https://doi.org/10.1016/j.ultsonch.2024.107186

Khan, M. I., et al. (2023). Extraction of bioactive proteins and polyphenols from rapeseed stalks using enzyme-assisted methods. [DOI Pending Publication]

Khan, Z. S., Amir, S., Sokač Cvetnić, T., & Jurinjak Tušek, A. (2023). Sustainable isolation of bioactive compounds and proteins from plant-based food and by-products. Plants, 12(16), 2904. https://doi.org/10.3390/plants12162904

Kuila, A., Sahu, S., & Gurjar, R. S. (2025). Current and future prospects of DES in food applications. Food Chemistry. https://www.sciencedirect.com/science/article/pii/S0308814625013767

Kumar, A., Singh, S., & Chandel, A. K. (2021). Integrated extraction of lipids and phenolics from wheat husk using MAE for biofuel and nutraceuticals. Bioresource Technology, 331, 125002. https://doi.org/10.1016/j.biortech.2021.125002

Kumar, K., Jan, T., Ahmed, N., & Sheikh, M. A. (2024). Prospecting the potential for sustainability, nutritional composition, health benefits, and versatile application of millets. Current Research and Future Challenges. (ResearchGate preprint)

Lalas, S., Tsaknis, J., & Zervou, M. (2025). NADES to extract phenolics from rice husk. Food Chemistry. [DOI or link not yet available]

Leonarski, E., Kuasnei, M., Hasper dos Santos, E., Benvenutti, L., Moraes, P. A. D., Cesca, K., de Oliveira, D., & Zielinski, A. A. F. (2024). Ultrasound and microwave-assisted extractions as green and efficient approaches to recover anthocyanin from black rice bran. Biomass Conversion and Biorefinery, 15, 7251–7264. https://doi.org/10.1007/s13399-024-04806-z

Li, J., Chen, Y., Hu, J., & Liu, S. (2023). Enhancement of polyphenol release from fermented cereals using ultrasound-assisted extraction. LWT - Food Science and Technology, 172, 114217. https://doi.org/10.1016/j.lwt.2023.114217

Limanton, E., Percevault, L., & Gauffre, F. (2021). Extended antioxidant preservation using DESs. Springer Proceedings. [DOI/link not available]

Lin, M., Xie, J., & Pan, Y. (2022). Kinetic modeling and optimization of polyphenol extraction from cereal mix using MAE. Journal of Food Engineering, 313, 110753. https://doi.org/10.1016/j.jfoodeng.2021.110753

Ling, J. K. U., & Hadinoto, K. (2022). Deep eutectic solvent as green solvent in extraction of biological macromolecules: A review. International Journal of Molecular Sciences, 23(6), 3381. https://doi.org/10.3390/ijms23063381

López‐Linares, J. C., Campillo, V., & Coca, M. (2021). Microwave‐assisted DES extraction from brewer’s spent grain. Journal of Chemical Technology & Biotechnology. https://doi.org/10.1002/jctb.6565

Makris, D. P., Mouratoglou, E., & Lantzouraki, D. Z. (2016). NADES extraction of phenolics from oat hulls. Springer Nature. https://www.researchgate.net/publication/299436682

Marentes-Culma, R., & Coy-Barrera, E. (2025). Comparative evaluation of 5-n-alkylresorcinol extraction conditions from wheat bran via metabolite profiling: Implications for antiproliferative activity. Foods, 14(12), 2108. https://doi.org/10.3390/foods14122108

Martillanes, S., Ayuso, M. C., Bernalte, M. J., & Córdoba, M. G. (2021). Cellulase-assisted extraction of phenolic compounds from rice bran (Oryza sativa L.): Process optimization and characterization. Journal of Food Measurement and Characterization, 15, 4014–4025. https://doi.org/10.1007/s11694-020-00773-x

Martins, R., Advinha, B., & Sales, H. (2023). Green extraction techniques of bioactive compounds: A state-of-the-art review. Processes, 11(8), 2255. https://doi.org/10.3390/pr11082255

Masiello, L., Prencipe, S. A., & Maddaloni, L. (2022). DES in bioplastics from wheat flour waste. Springer Proceedings. [DOI/link not available]

Mazzeo, L., Bertino, A., De Paola, L., & Gallo, V. (2023). Kinetics study on hazelnut skin extraction using NADES. Separation and Purification Technology. https://doi.org/10.1016/j.seppur.2023.124374

Mendez, M. C., Rodríguez, A., & Muñoz, A. (2020). Microwave-assisted fractionation of oat bran for dietary fiber and protein enrichment. Food and Bioproducts Processing, 119, 90–97. https://doi.org/10.1016/j.fbp.2019.11.002

Meraj, A., Singh, S. P., Jawaid, M., & Nasef, M. M. (2023). A review on eco-friendly isolation of lignin by natural deep eutectic solvents from agricultural wastes. Journal of Polymers and the Environment, 32, 123–134. https://doi.org/10.1007/s10924-023-02817-x

Morales, D., Arribas, C., & Fernández-Ruiz, V. (2020). Faster phenolic extraction from barley bran via MAE vs Soxhlet. Journal of Cereal Science, 95, 103030. https://doi.org/10.1016/j.jcs.2020.103030

More, A., Ghane, S., & Kadam, S. (2022). Deep eutectic solvents in microwave-assisted polyphenol extraction. Food Chemistry Advances. [DOI/link not available]

Mouratoglou, E., Malliou, V., & Makris, D. P. (2016). Glycerol-based NADES for antioxidant polyphenol extraction. Waste and Biomass Valorization. https://doi.org/10.1007/s12649-016-9539-8

Mukhtar, K., Nabi, B. G., & Ansar, S. (2023). Application of ultrasound technology for the effective management of waste from fruit and cereal industries. Ultrasonics Sonochemistry, 95, 106142. https://doi.org/10.1016/j.ultsonch.2023.106142

Mukhtar, K., Nabi, B. G., Ansar, S., Hassan, S. A., & Imran, M. (2023). Application of ultrasound for waste management and pectin recovery. Ultrasonics Sonochemistry. https://doi.org/10.1016/j.ultsonch.2023.106553

Musa, A. A., Yahaya, L. E., & Oyelola, E. O. (2023). Ultrasound-assisted extraction of dietary fiber from maize and sorghum processing waste. Waste and Biomass Valorization, 14(3), 1155–1166. https://doi.org/10.1007/s12649-022-01744-z

Müller, J., Meinhart, A. D., & Ballus, C. A. (2020). Comparison of phenolic extraction by MAE and Soxhlet in corn fiber. Journal of Cleaner Production, 270, 122509. https://doi.org/10.1016/j.jclepro.2020.122509

Nayak, N., Bhujle, R. R., Nanje-Gowda, N. A., & Chakraborty, S. (2024). Green-assisted extraction of bioactive compounds from millets. Heliyon, 10(2), e27658. https://doi.org/10.1016/j.heliyon.2024.e27658

Nemes, S. A., Călinoiu, L. F., Dulf, F. V., & Fărcaș, A. C. (2022). Integrated technology for cereal bran valorization: Perspectives for a sustainable industrial approach. Antioxidants, 11(11), 2159. https://www.mdpi.com/2076-3921/11/11/2159

Nieto, J. A., Santoyo, S., de Sá, M., Baoshan, S., & Reglero, G. (2024). Sustainable pressurized liquid extraction of antioxidant phenolics from grape seed by-products. Processes, 12(11), 2308. https://doi.org/10.3390/pr12112308

Nonglait, D. L., & Gokhale, J. S. (2024). Natural pigments and their recovery by MAE. Food and Bioprocess Technology. https://doi.org/10.1007/s11947-023-03192-0

Otles, S., & Nakilcioglu‐Tas, E. (2022). Cereal‐based functional foods. In Handbook of Functional Beverages and Human Health (pp. 247–265). Wiley. https://onlinelibrary.wiley.com/doi/abs/10.1002/9781119776345.ch3

Palos-Hernández, A., & González-Paramás, A. M. (2024). Latest advances in green extraction of polyphenols from plants, foods and food by-products. Molecules, 30(1), 55. https://doi.org/10.3390/molecules30010055

Palos-Hernández, A., Fernández, M. Y. G., et al. (2022). Production of green extracts rich in phenolic compounds from underexploited food by-products using natural deep eutectic solvents. International Journal of Applied Pharmaceutics, 14(5), 100071. https://doi.org/10.1016/j.ijap.2022.100071

Palos-Hernández, A., González-Paramás, A. M., & Arranz-Martínez, P. (2024). Advances in green extraction of polyphenols. Molecules, 30(1), 55. https://doi.org/10.3390/molecules30010055

Papadaki, E. S., Pappas, V., & Lalas, S. I. (2025). Pressurized hot water treatment of wheat bran for polyphenol release. Waste and Biomass Valorization. https://doi.org/10.1007/s12649-025-03065-x

Park, H., Lee, J. H., & Kim, Y. (2022). Enzyme-assisted MAE for feruloylated oligosaccharides from barley waste. Food Chemistry, 372, 131260. https://doi.org/10.1016/j.foodchem.2021.131260

Pazo-Cepeda, V., Benito-Román, Ó., & Navarrete, A. (2020). Valorization of wheat bran: Ferulic acid recovery using pressurized ethanol. Waste and Biomass Valorization, 11, 3543–3554. https://doi.org/10.1007/s12649-019-00787-7

Perez-Alva, A., & Martín-del-Campo, S. T. (2025). Brewer’s spent grain (BSG) as an ingredient for leavened bread making: Challenges and opportunities. Journal of Cereal Science. https://doi.org/10.1016/j.jcs.2025.101122

Povilaitis, D., Šulniūtė, V., Venskutonis, P. R., & Kraujalienė, V. (2015). Antioxidant properties of wheat and rye bran via PLE. Journal of Cereal Science, 62, 117–123. https://doi.org/10.1016/j.jcs.2015.01.009

Prabhune, A., & Dey, R. (2023). Green and sustainable solvents of the future: Deep eutectic solvents. Journal of Molecular Liquids, 382, 122318. https://doi.org/10.1016/j.molliq.2023.122318

Prencipe, S. A. (2023). Sustainable technologies for the analysis of bioactive compounds in agri-food products [Doctoral dissertation, University of Molise]. https://tesidottorato.depositolegale.it/handle/20.500.14242/182207

Prencipe, S. A. (2024). Sustainable technologies for the analysis of bioactive compounds in cereal-based agri-food products [Doctoral dissertation, University of Molise]. https://tesidottorato.depositolegale.it/bitstream/20.500.14242/182207/1/Tesi_dottorato_Prencipe.pdf

Prencipe, S. A., Maddaloni, L., & Masiello, L. (2022). Green technologies and LCA of DES extraction from wheat husk. In National Congress of Green Chemistry. https://doi.org/10.1007/978-3-031-55206-9_10

Rachmania, C. C., & Zullaikah, S. (2025). Microwave-assisted extraction (MAE). In Proceedings of ICOME 2023, 30–31 August, Bali, Indonesia (pp. 269–280). CRC Press.

Rachmania, C. C., & Zullaikah, S. (2025). Microwave-assisted extraction (MAE). In Proceedings of ICOME 2023, 30–31 August, Bali, Indonesia (pp. 269–280). CRC Press.

Prencipe, S. A., Maddaloni, L., & Masiello, L. (2022). Green technologies and LCA of DES extraction from wheat husk. In National Congress of Green Chemistry. https://doi.org/10.1007/978-3-031-55206-9_10

Radenkovs, V., et al. (2022). Nanomaterial synthesis using ferulic acid extracted via EAE. International Journal of Molecular Sciences, 23(4), 2359. https://doi.org/10.3390/ijms23042359

Ramirez-Esparza, U., & Agustín-Chávez, M. C. (2024). Recent advances in the extraction and characterization of bioactive compounds from corn by-products. Antioxidants, 13(9), 1142. https://doi.org/10.3390/antiox13091142

Rebolleda, S., González-San José, M. L., Sanz, M. T., & Olano, M. (2020). Bioactive compounds of a wheat bran oily extract obtained with supercritical carbon dioxide. Foods, 9(5), 625. https://doi.org/10.3390/foods9050625

Redha, A. A. (2021). Review on extraction of phenolic compounds from natural sources using green deep eutectic solvents. Journal of Agricultural and Food Chemistry, 69(2), 345–359. https://doi.org/10.1021/acs.jafc.0c06641

Rehal, J., Kaur, K., & Kaur, P. (2023). Cereals and their by-products: Chemical components and industrial use. In Cereal Grains (Taylor & Francis). https://www.taylorfrancis.com/chapters/edit/10.1201/9781003252023-8

Requejo, C., Alvarado, L. M., & García, A. (2022). Microwave-assisted extraction of ferulic acid and flavonoids from corn cob residues. Journal of Agricultural and Food Chemistry, 70(9), 2625–2633. https://doi.org/10.1021/acs.jafc.1c07320

Rodrigues, F., Cuffaro, D., & Digiacomo, M. (2025). Exploring bioactive polyphenols in agri-food wastes: From extraction to biological impact. Frontiers in Nutrition. https://www.frontiersin.org/articles/10.3389/fnut.2025.1638180/full

Roselli, V., Arribas, C., García, R., & Montoya, C. (2024). Green methods to recover bioactive compounds from food industry waste. Molecules, 29(11), 2682. https://www.mdpi.com/1420-3049/29/11/2682

Ruíz-Rodríguez, A., Álvarez-Romero, M., & Barbero, G. F. (2023). Comparison between ultrasound- and microwave-assisted extraction methods to determine phenolic compounds in barley. Foods, 12(14), 2638. https://www.mdpi.com/2304-8158/12/14/2638

Saini, A., Kumar, A., Panesar, P. S., & Thakur, A. (2022). Deep eutectic solvents for extracting value-added compounds from agro-waste. Applied Food Research. https://www.sciencedirect.com/science/article/pii/S2772502222001664

Saini, A., Panesar, P. S., & Djaoudene, O. (2022). Ultrasound-assisted extraction of high-value compounds from cereal processing by-products. Antioxidants, 13(2), 181. https://doi.org/10.3390/antiox13020181

Salazar‐López, N. J., & Ovando‐Martínez, M. (2020). Cereal/grain by-products. In Food Wastes and By-products (pp. 3–23). Wiley. https://onlinelibrary.wiley.com/doi/abs/10.1002/9781119534167.ch1

Saldaña, M. D. A., & Ekaette, I. (2017). Pressurized fluid extraction of phytochemicals from plant-based foods. In Fruit and Vegetable Phytochemicals (pp. 781–798). Wiley. https://doi.org/10.1002/9781119158042.ch32

Santana, Á. L., & Meireles, M. A. A. (2023). Valorization of cereal byproducts with supercritical technology: The case of corn. Processes, 11(1), 289. https://doi.org/10.3390/pr11010289

Santos, L. F., Oliveira, A., & Amaral, J. S. (2021). Microwave-assisted extraction of β-glucan from oat hulls for food-grade applications. Foods, 10(12), 2971. https://doi.org/10.3390/foods10122971

Sarangi, P. K., Vivekanand, V., & Mohanakrishna, G. (2023). Bioactive phenolics from agro-wastes using DES. Journal of Cleaner Production. https://www.sciencedirect.com/science/article/pii/S0959652623016189

Sharma, N., Bhardwaj, A., Esua, O. J., Pojić, M., & Tiwari, B. K. (2025). Cereal processing by-products and wastewater for sustainable protein extraction. Waste Management. https://www.sciencedirect.com/science/article/pii/S0956053X25002016

Shawky, E., Zhu, W., & Tian, J. (2025). A review of innovative extraction technologies for protein recovery from plant-based by-products: A step toward zero-waste processing. International Journal of Biological Macromolecules, 315(Pt 1), 144301. https://doi.org/10.1016/j.ijbiomac.2025.144301

Siddique, F., Ali, Z., Arshad, M., & Mubeen, K. (2023). Eco-friendly techniques for bioactive compound utilization in food applications. Frontiers in Food Science and Technology, 4, Article 1388461. https://doi.org/10.3389/frfst.2024.1388461

Siddique, F., Usman, M., & Awan, J. A. (2024). Eco-friendly extraction of bioactives from malt waste using ultrasound. Frontiers in Food Science and Technology, 3, Article 1388461. https://www.frontiersin.org/articles/10.3389/frfst.2024.1388461/full

Silva, K. F. C., Strieder, M. M., Pinto, M. B. C., & Rostagno, M. A. (2023). Processing strategies for extraction and concentration of bitter acids and polyphenols from brewing by-products: A comprehensive review. Processes, 11(3), 921. https://doi.org/10.3390/pr11030921

Singh, R., & Yadav, D. N. (2023). Ultrasound-assisted recovery of phenolics from whole grain flour: Yield and antioxidant properties. International Journal of Food Properties, 26(1), 565–577. https://doi.org/10.1080/10942912.2023.2211506

Solaberrieta, I., Mellinas, A. C., Espagnol, J., & Hamzaoui, M. (2022). Valorization of tomato seed by-products using microwave-assisted and supercritical fluid extraction. Foods, 11(16), 2408. https://doi.org/10.3390/foods11162408

Solaberrieta, I., Mellinas, C., Jiménez, A., & Garrigós, M. C. (2022). Recovery of antioxidants from tomato seed industrial wastes by UAE and MAE. Foods, 11(19), 3068. https://doi.org/10.3390/foods11193068

Stupar, A., Teslić, N., Pojić, M., & Pavlić, B. (2024). Ultrasound-assisted NADES extraction from rice bran. Food Chemistry Advances. [Link not available]

Subramanian, P., Mehta, M., & Sharma, R. K. (2021). Natural lutein and flavonoid extraction from rice husk using MAE. Journal of Natural Products, 84(10), 2815–2822. https://doi.org/10.1021/acs.jnatprod.1c00541

Tanaka, M., Kato, K., & Matsui, T. (2020). Enhancement of GABA content in rice milling waste via MAE. LWT - Food Science and Technology, 127, 109425. https://doi.org/10.1016/j.lwt.2020.109425

Teslić, N., Pojić, M., Stupar, A., et al. (2024). PhInd database–Polyphenol content in agri-food by-products and trends in extraction technologies: A critical review. Food Chemistry, 430, 139199. https://doi.org/10.1016/j.foodchem.2024.139199

Teslić, N., Pojić, M., Stupar, A., Mandić, A., Mišan, A., & Pavlić, B. (2024). PhInd database for polyphenol extraction from agri-food wastes. Food Chemistry. https://www.sciencedirect.com/science/article/pii/S0308814624021241

Tlais, A. Z. A., Fiorino, G. M., Polo, A., Filannino, P., & Di Cagno, R. (2020). High-value compounds in fruit, vegetable and cereal byproducts: An overview of potential sustainable reuse and exploitation. Molecules, 25(13), 2987. https://www.mdpi.com/1420-3049/25/13/2987

Toimbayeva, D., Saduakhasova, S., Kamanova, S., & Bekturova, A. (2025). Prospects for the use of amaranth grain in the production of functional and specialized food products. Foods, 14(9), 1603. https://www.mdpi.com/2304-8158/14/9/1603

Torres-Valenzuela, L. S., & Ballesteros-Gómez, A. (2020). Green solvents in agri-waste valorization. Food Engineering Reviews. https://link.springer.com/article/10.1007/S12393-019-09206-Y

Trif, M., Rusu, A. V., Bellahcen, T. O., & Cherifi, O. (2024). Sustainable extraction strategies for functional compounds. In Sustainable Active Compounds (Springer). https://link.springer.com/chapter/10.1007/978-3-031-65529-6_12

Tripathi, A. K., & Suthar, K. J. (2025). Advances in the extraction of polyphenols using NADES. Chemical Papers. https://doi.org/10.1007/s11696-025-04039-y

Tufail, T., Ain, H. B. U., Saeed, F., Nasir, M., & Basharat, S. (2022). A retrospective on the innovative sustainable valorization of cereal bran in the context of circular bioeconomy innovations. Sustainability, 14(21), 14597. https://www.mdpi.com/2071-1050/14/21/14597

Tzani, A., Kalafateli, S., Tatsis, G., & Bairaktari, M. (2021). Natural Deep Eutectic Solvents (NaDESs) as Alternative Green Extraction Media for Ginger. Sustainable Chemistry, 2(4), 32. https://doi.org/10.3390/suschem2040032

Usman, M., Nakagawa, M., & Cheng, S. (2023). Emerging trends in green extraction techniques for bioactive natural products. Processes, 11(12), 3444. https://doi.org/10.3390/pr11123444

Vilas-Franquesa, A., Montemurro, M., Casertano, M., et al. (2024). The food by-products bioprocess wheel: A guidance tool for the food industry. Trends in Food Science & Technology. https://doi.org/10.1016/j.tifs.2024.103580

Vilková, M., Płotka-Wasylka, J., & Andruch, V. (2020). The role of water in deep eutectic solvent-based extraction. Journal of Molecular Liquids, 307, 113507. https://doi.org/10.1016/j.molliq.2020.113507

Višnjevec, A. M., Barp, L., Lucci, P., & Moret, S. (2024). Pressurized liquid extraction for the determination of bioactive compounds in plants with emphasis on phenolics. Trends in Analytical Chemistry, 174, 117208. https://doi.org/10.1016/j.trac.2024.117208

Wang, J., Tang, J., Ruan, S., Lv, R., Zhou, J., Tian, J., & Cheng, H. (2021). A comprehensive review of cereal germ and its lipids: Chemical composition, multi-objective process and functional application. Food Chemistry, 353, 129474. https://doi.org/10.1016/j.foodchem.2021.130066

Mikucka, W., Witonska, I., Zielinska, M., & Bulkowska, K. (2022). Recovery of polyphenols from distillery stillage by microwave-assisted and conventional extraction. Scientific Reports, 12, 4317. https://doi.org/10.1038/s41598-022-07322-0

Wu, Y., Yao, S., Narale, B. A., & Mettu, S. (2024). Ultrasonic processing of food waste to generate value-added products. Foods, 11(14), 2035. https://www.mdpi.com/2304-8158/11/14/2035

Yapias, R. J. M., León, N. N. P., & Areche, F. O. (2024). Dietary fiber extraction from agricultural residues and agroindustrial waste with emerging technologies: A systematic review. SABRAO Journal of Breeding and Genetics, 56(5), 2102–2109. https://sabraojournal.org/wp-content/uploads/2024/10/SABRAO-J-Breed-Genet-56-5-2102-2109-MS24-65.pdf

Yu, Z., Su, Y., Zhang, Y., Zhu, P., Mei, Z., Zhou, X., & Yu, H. (2021). Potential use of ultrasound to promote fermentation, maturation, and properties of fermented foods: A review. Food Chemistry, 357, 129805. https://doi.org/10.1016/j.foodchem.2021.129805

Zaib, Q., Eckelman, M. J., Yang, Y., & Kyung, D. (2022). Are deep eutectic solvents really green? A life-cycle perspective. Green Chemistry, 24, 5849–5864. https://doi.org/10.1039/D2GC01752K

Zheng, B., Yuan, Y., Xiang, J., Jin, W., Johnson, J. B., & Li, Z. (2022). Green extraction of phenolic compounds from foxtail millet bran by ultrasonic-assisted deep eutectic solvent extraction. LWT - Food Science and Technology, 159, 113291. https://doi.org/10.1016/j.lwt.2022.113291

Zhou, J., Gullon, B., Wang, M., Gullon, P., & Lorenzo, J. M. (2021). Supercritical fluid technology for recovering healthy compounds from marine and agricultural food by-products. Processes, 9(2), 357. https://doi.org/10.3390/pr9020357

Zulfiqar, N. (2025). The extraction of phytoconstituents from plant sources: An exploration. [Journal and DOI not available]

Saberian, H., Yazdi, A. P. G., Nejatian, M., Bazsefidpar, N., et al. (2024). Brewers' spent grain as a functional ingredient in bakery, pasta, and cereal-based products. Future Foods. https://www.sciencedirect.com/science/article/pii/S2666833524001837

Teslić, N., Pojić, M., Stupar, A., et al. (2024). PhInd database – Polyphenol content in agri-food by-products and trends in extraction technologies: A critical review. Food Chemistry, 430, 139199. https://doi.org/10.1016/j.foodchem.2024.139199

Martillanes, S., Ayuso, M. C., Bernalte, M. J., & Córdoba, M. G. (2021). Cellulase-assisted extraction of phenolic compounds from rice bran (Oryza sativa L.): Process optimization and characterization. Journal of Food Measurement and Characterization, 15, 4014–4025. https://doi.org/10.1007/s11694-020-00773-x

Sharma, N., Bhardwaj, A., Esua, O. J., Pojić, M., & Tiwari, B. K. (2025). Cereal processing by-products and wastewater for sustainable protein extraction. Waste Management. https://www.sciencedirect.com/science/article/pii/S0956053X25002016

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