Antifungal Activity of Methanol Extracts from Verbascum Species Collected from Different Regions Against Rhizoctonia solani
DOI:
https://doi.org/10.24925/turjaf.v14i2.351-355.8118Keywords:
Antifungal activity, Rhizoctonia solani, Verbascum spp., Methanol extract, Natural fungicideAbstract
Plants are considered important natural resources in plant pathogen management due to the secondary metabolites they contain. In this study, the antifungal effects of methanol extracts obtained from Verbascum (mullein) species collected from ecologically diverse locations [Sakarya (Arifiye, Karasu), Konya, Bolu, and Manisa] were investigated under in vitro conditions against the soil-borne phytopathogenic fungus Rhizoctonia solani (J.G. Kühn), which has a broad host range. Methanol extracts were prepared from the flower and leaf parts of Verbascum species, and their potential to inhibit mycelial growth was evaluated at different concentrations (0.5%, 1%, 2%, and 4%) in culture medium. All extracts exhibited antifungal activity at varying levels. The highest inhibition was observed in the extract from Konya (55%), followed by the Karasu extract (45%). Extracts from other locations showed inhibition rates ranging between 7.5% and 36.25%. The findings demonstrate that methanol extracts of Verbascum species possess antifungal activity against R. solani, and that the effect increases in a dose-dependent manner. These results suggest that the active compounds contained in Verbascum species should be identified and further investigated for their potential as alternative natural fungicides in biological control.
References
Bahraminejad, S. (2012). In vitro and in vivo antifungal activities of Iranian plant species against Pythium aphanidermatum. Annals of Biological Research, 3(5), 2134–2143.
Beşen, B. S., Karahan, L. E., Parlakyiğit, P., Kırılmış, C., & Erdoğan, S. (2022). Bazı bitki kaynaklarının farklı çözgenlerde hazırlanan ekstrelerinin antimikrobiyal aktivitesinin araştırılması. Çukurova Üniversitesi Mühendislik Fakültesi Dergisi, 37(2), 543–553. https://doi.org/10.21605/cukurovaummfd.1040474
Bhandari, S., Yadav, P. K., & Sarhan, A. (2021). Botanical fungicides; current status, fungicidal properties and challenges for wide scale adoption: A review. Reviews in Food and Agriculture, 2(2), 63–68. https://doi.org/10.26480/rfna.02.2021.63.68
Ceruso, M., Clement, J. A., Todd, M. J., Zhang, F., Huang, Z., Anastasio, A., … & Liu, Y. (2020). The inhibitory effect of plant extracts on growth of the foodborne pathogen, Listeria monocytogenes. Antibiotics, 9(6), 319. https://doi.org/10.3390/antibiotics9060319
Deans, S. G., & Svoboda, K. P. (1990). The antimicrobial properties of marjoram (Origanum majorana L.) volatile oil. Flavour and Fragrance Journal, 5(3), 187–190. https://doi.org/10.1002/ffj.2730050310
Fletcher, J., Bender, C., Budowle, B., Cobb, W. T., Gold, S. E., Ishimaru, C. A., … & Tolin, S. A. (2006). Plant pathogen forensics: Capabilities, needs, and recommendations. Microbiology and Molecular Biology Reviews, 70(2), 450–471. https://doi.org/10.1128/MMBR.00022-05
Gupta, S., Bhaskar, G., & Andola, C. H. (2011). Altitudinal variation in essential oil content in leaves of Zanthoxylum alatum a high value aromatic tree from Uttarakhand. Research Journal of Medicinal Plant, 5(3), 348–351. https://doi.org/10.3923/rjmp.2011.348.351
Kadıoğlu, İ., & Yanar, Y. (2004). Allelopathic effects of plant extracts against seed germination of some weeds. Asian Journal of Plant Sciences, 3(4), 472–475. https://doi.org/10.3923/ajps.2004.472.475
Khan, A. M., Qureshi, R. A., Gillani, S. A., & Ullah, F. (2011). Antimicrobial activity of selected medicinal plants of Margalla Hills, Islamabad, Pakistan. Journal of Medicinal Plants Research, 5(18), 4665–4670.
Kılıç, M., & Kılıç, F. M. (2025). Comparative anatomy of some Verbascum taxa (Scrophulariaceae) in Southeastern Anatolia and distinguishing characteristics of these taxa. Celal Bayar University Journal of Science, 21(1), 1–19. https://doi.org/10.18466/cbayarfbe.1234567
Mohamed, N. H., & El-Hadidy, A. M. (2008). Studies of biologically active constituents of Verbascum eremobium Murb. and its inducing resistance against some diseases of cucumber. Egyptian Journal of Phytopathology, 36(1–2), 133–150.
Moore, D., Robson, G. D., & Trinci, A. P. (2020). 21st century guidebook to fungi. Cambridge University Press. https://doi.org/10.1017/9781316339732
Nowak, M., Kleinwaechter, M., Manderscheid, R., Weigel, H. J., & Selmar, D. (2010). Drought stress increases the accumulation of monoterpenes in sage (Salvia officinalis), an effect that is compensated by elevated carbon dioxide concentration. Journal of Applied Botany and Food Quality, 83(2), 133–136.
Onaran, A., & Yılar, M. (2012). Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. Journal of Food, Agriculture & Environment, 10(3–4), 287–291.
Radušienė, J., Karpavičienė, B., & Stanius, Ž. (2012). Effect of external and internal factors on secondary metabolite accumulation in St. John’s wort. Botanica Lithuanica, 18(2), 101–108. https://doi.org/10.2478/v10279-012-0014-2
Sadeqifard, S., Mirmostafaee, S., Joharchi, M. R., Zandavifard, J., Azizi, M., & Fujii, Y. (2022). Evaluation of allelopathic activity interactions of some medicinal plants using fractional inhibitory concentration and isobologram. Agronomy, 12(12), 3001. https://doi.org/10.3390/agronomy12123001
Salman, Ö., & Boyraz, N. (2023). Efficacy of some Trichoderma isolates as biocontrol agents against Rhizoctonia solani Kühn in bean (Phaseolus vulgaris L.). Selcuk Journal of Agriculture and Food Sciences, 37(1), 133–144.
Saltan, F. Z., Sokmen, M., Akin, M., Saracoglu, H. T., Gokturk, R. S., Ahmad, M., … & Shah, M. R. (2011). Antimicrobial and antioxidant activities of phenolic compound extracted from new Verbascum species growing in Turkey. Journal of the Chemical Society of Pakistan, 33(5), 764–771.
Selseleh, M., Ebrahimi, S. N., Aliahmadi, A., Sonboli, A., & Mirjalili, M. H. (2020). Metabolic profiling, antioxidant, and antibacterial activity of some Iranian Verbascum L. species. Industrial Crops and Products, 153, 112609. https://doi.org/10.1016/j.indcrop.2020.112609
Şin, B., Kadıoğlu, İ., & Onaran, A. (2017). Parazit bitkilerden (Orobanche ramosa L., Cuscuta campestris Yunck. ve Viscum album L.) elde edilen ekstraktların bazı bitki patojeni funguslara karşı antifungal etkileri. Turkish Journal of Weed Science, 20(1), 61–69.
Tariq, A., Shah, G. M., Ahmad Zada, A. A., Shah, A. Z., & Fatima, I. (2021). Phytochemical analysis and in vitro antibacterial and antifungal activity of Verbascum arianthum (Benth). Pure and Applied Biology, 10(3), 797–806. https://doi.org/10.19045/bspab.2021.100084
Tatli, I. I., & Akdemir, Z. F. (2006). Traditional uses and biological activities of Verbascum species. FABAD Journal of Pharmaceutical Sciences, 31(2), 85–96.
Verma, N., & Shukla, S. (2015). Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4), 105–113. https://doi.org/10.1016/j.jarmap.2015.09.002
Vogt, M. V., Cravero, C., Tonn, C. E., Sabini, L. I., & Rosas, S. B. (2010). Verbascum thapsus: Antifungal and phytotoxic properties. Idecefyn, 20(1), 105–108.
Yang, L., Wen, K. S., Ruan, X., Zhao, Y. X., Wei, F., & Wang, Q. (2018). Response of plant secondary metabolites to environmental factors. Molecules, 23(4), 762. https://doi.org/10.3390/molecules23040762
Zengin, G., Mahomoodally, M. F., Sinan, K. I., Sadeer, N., Maggi, F., Caprioli, G., … & Akan, H. (2021). Evaluation of chemical constituents and biological properties of two endemic Verbascum species. Process Biochemistry, 108, 110–120. https://doi.org/10.1016/j.procbio.2021.06.004
Zobayed, S. M. A., Afreen, F., & Kozai, T. (2005). Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s wort. Plant Physiology and Biochemistry, 43(10–11), 977–984. https://doi.org/10.1016/j.plaphy.2005.08.010
Zubrod, J. P., Bundschuh, M., Arts, G., Brühl, C. A., Imfeld, G., Knäbel, A., Payraudeau, S., Rasmussen, J. J., Rohr, J., Scharmüller, A., Smalling, K. L., Stehle, S., Schulz, R., & Schäfer, R. B. (2019). Fungicides: An overlooked pesticide class? Environmental Science & Technology, 53(7), 3347–3365. https://doi.org/10.1021/acs.est.8b04392
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