Cloud Point Extraction for the Recovery of Bioactive Compounds from Peanut Shells

Authors

DOI:

https://doi.org/10.24925/turjaf.v14i1.103-108.8189

Keywords:

Peanut shell, bioactive compounds, cloud point extraction, Agricultural waste , Sustainability

Abstract

This study investigated the effectiveness of the cloud point extraction (CPE) method for the recovery of bioactive phenolic and flavonoid compounds from peanut shells. Key parameters affecting extraction efficiency, including salt concentration, pH, temperature, and sample amount, were systematically evaluated. The findings revealed that the optimal salt concentration for higher total phenolic content was 10%, whereas the maximum yield of total flavonoid compounds was obtained at a 12% salt concentration. Beyond these concentrations, a decline in extraction efficiency was observed for both compound groups. In terms of pH, the highest recovery of total phenolics occurred at pH 4, while total flavonoids reached their peak at pH 4.5. Overall, acidic conditions were found to enhance extraction performance. Regarding temperature, both phenolic and flavonoid yields were maximized at 90 °C. For the sample amount, the optimum recovery was achieved with 0.05 grams, while higher sample quantities led to decreased efficiency. These results underscore the critical importance of precisely optimizing CPE conditions to ensure efficient extraction of phenolic and flavonoid compounds from peanut shell biomass.

References

Arya, S. S., Salve, A. R., & Chauhan, S. (2016). Peanuts as functional food: a review. Journal of Food Science and Technology, 53, 31-41.

Bertioli, D. J., Seijo, G., Freitas, F. O., Valls, J. F., Leal-Bertioli, S. C., & Moretzsohn, M. C. (2011). An overview of peanut and its wild relatives. Plant Genetic Resources, 9(1), 134-149.

Çiftçi, S., & Suna, G. Ü. L. E. N. (2022). Functional components of peanuts (Arachis hypogaea L.) and health benefits: A review. Future foods, 5, 100140.

Daigle, D. J., Conkerton, E. J., Sanders, T. H., Mixon, A. C. (1988). Peanut hull flavonoids: Their relationship with peanut maturity. Journal of Agricultural and Food Chemistry, 36(6), 1179-1181.

De Araújo Padilha, C. E., de Azevedo, J. C. S., de Sousa Júnior, F. C., de Oliveira Júnior, S. D., de Santana Souza, D. F., de Oliveira, J. A., ... & dos Santos, E. S. (2018). Recovery of polyphenols from camu-camu (Myrciaria dubia HBK McVaugh) depulping residue by cloud point extraction. Chinese Journal of Chemical Engineering, 26(12), 2471-2476.

Dean, L. L. (2020). Extracts of peanut skins as a source of bioactive compounds: methodology and applications. Applied Sciences, 10(23), 8546.

Duh, P. D., & Yen, G. C. (1995). Changes in antioxidant activity and components of methanolic extracts of peanut hulls irradiated with ultraviolet light. Food Chemistry, 54(2), 127-131.

Duh, Pin Der, Yeh, D. B., Yen, G. C. (1992). Extraction and identification of an antioxidative component from peanut hulls. Journal of the American Oil Chemists Society, 69(12), 1277-1277.

El-Abbassi, A., Kiai, H., Raiti, J., & Hafidi, A. (2014). Cloud point extraction of phenolic compounds from pretreated olive mill wastewater. Journal of Environmental Chemical Engineering, 2(3), 1480-1486.

Er, B. (2021). Ispanaktan lutein ve β-karotenin bulutlanma noktası tekniği ile ekstraksiyonu (Yüksek lisans tezi). İnönü Üniversitesi, Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı, Malatya, Türkiye.

FAOSTAT, (2020). http://www.fao.org/faostat/en/#data/QCL, Erişim tarihi: 12.02.2025

Freeland-Graves, J. H., & Nitzke, S. (2013). Position of the academy of nutrition and dietetics: total diet approach to healthy eating. Journal of the Academy of Nutrition and Dietetics, 113(2), 307-317.

Gökkaya, N. (2014). Bazı Eser Metallerin Bulutlanma Noktası Ekstraksiyonu İle Tayini(Yüksek Lisans Tezi). Pamukkale Üniversitesi, Fen Bilimleri Enstitüsü, Denizli.

Guo, Y., Liu, Y., Zhang, Z., Chen, M., Zhang, D., Tian, C., ... & Jiang, G. (2020). The antibacterial activity and mechanism of action of luteolin against Trueperella pyogenes. Infection and drug resistance, 1697-1711.

Imran, A., Humiyion, M., Arshad, M. U., Saeed, F., Arshad, M. S., Afzaal, M., ... & Al Jbawi, E. (2022). Extraction, amino acid estimation, and characterization of bioactive constituents from peanut shell through eco-innovative techniques for food application. International Journal of Food Properties, 25(1), 2055-2065.

Ji, Y., Wu, L., Lv, R., , H., Song, S., & Cao, M. (2021). Facile cloud point extraction for the separation and determination of phenolic acids from dandelion. ACS omega, 6(20), 13508-13515.

Jung, M., Kim, J., & Ahn, S. M. (2020). Factors associated with frequency of peanut consumption in Korea: a national population-based study. Nutrients, 12(5), 1207.

Katsoyannos, E., Chatzilazarou, A., Gortzi, O., Lalas, S., Konteles, S., & Tataridis, P. (2006). Application of cloud point extraction using surfactants in the isolation of physical antioxidants(phenols) from olive mill wastewater. Fresenius Environmental Bulletin, 15(9), 1122-1125.

Khani, R., Sheykhi, R., & Bagherzade, G. (2019). An environmentally friendly method based on micro-cloud point extraction for determination of trace amount of quercetin in food and fruit juice samples. Food Chemistry, 293, 220-225.

Kiai, H., Raiti, J., El-Abbassi, A., & Hafidi, A. (2018). Recovery of phenolic compounds from table olive processing wastewaters using cloud point extraction method. Journal of environmental chemical engineering, 6(1), 1569-1575.

Liu, W., Wang, L., & Zhang, J. (2022). Peanut Shell extract and Luteolin regulate lipid metabolism and induce Browning in 3T3-L1 adipocytes. Foods, 11(17), 2696.

Mai, X., Liu, Y., Tang, X., Wang, L., Lin, Y., Zeng, H., ... & Li, P. (2020). Sequential extraction and enrichment of flavonoids from Euonymus alatus by ultrasonic-assisted polyethylene glycol-based extraction coupled to temperature-induced cloud point extraction. Ultrasonics Sonochemistry, 66, 105073.

Mandala, R., Hegde, G., Kodali, D., Kode, V. R. (2023). From Waste to Strength: Unveiling the Mechanical Properties of Peanut-Shell-Based Polymer Composites. Journal of Composites Science, 7(8), 307.

Melnyk, A., & Namies, J. (2015). Theory and recent applications of coacervate-based extraction techniques. Trends in Analytical Chemistry, 71, 282–292.

Mishra, A. P., Saklani, S., Parcha, V., Nigam, M., & Coutinho, H. D. (2021). Antibacterial activity and phytochemical characterisation of Saussurea gossypiphora D. Don. Archives of Microbiology, 203(8), 5055-5065.

More, P. R., & Arya, S. S. (2019). A novel, green cloud point extraction and separation of phenols and flavonoids from pomegranate peel: An optimization study using RCCD. Journal of Environmental Chemical Engineering, 7(5), 103306.

More, P. R., Jambrak, A. R., & Arya, S. S. (2022). Green, environment-friendly and sustainable techniques for extraction of food bioactive compounds and waste valorization. Trends in Food Science & Technology, 128, 296-315.

Motikar, P. D., More, P. R., & Arya, S. S. (2021). A novel, green environment-friendly cloud point extraction of polyphenols from pomegranate peels: A comparative assessment with ultrasound and microwave-assisted extraction. Separation Science and Technology, 56(6), 1014-1025.

Niu, L., Zhang, S., Si, X., Fang, Y., Wang, S., Li, L., & Sheng, Z. (2025). Ultrasonic-assisted extraction of luteolin from peanut shells using ionic liquid and its molecular mechanism. Ultrasonics sonochemistry, 113, 107228.

Pendse, R., Rao, A. R., & Venkataraman, K. (1973). 5, 7-Dihydroxychromone from Arachis hypogoea shells. Phytochemistry, 12(8), 2033-2034.

Peng, M., Chen, Z., Deng, Q., Zhu, S., Wang, G. (2021). The roles of luteolin in peanut shell extract - Mediated protection of erythrocytes against hypoxanthine-xanthine oxidase-induced toxicity. Food Bioscience, 39, 100826.

Qiu, J., Chen, L., Zhu, Q., Wang, D., Wang, W., Sun, X., … Du, F. (2012). Screening natural antioxidants in peanut shell using DPPH–HPLC–DAD–TOF/MS methods. Food Chemistry, 135(4), 2366-2371.

Şahin, G. (2014). Türkiye'de Yerfıstığı (Arachis hypogaea L.) Yetiştiriciliği ve Bir Coğrafi İşaret Olarak Osmaniye Yerfıstığı. Gaziantep University Journal of Social Sciences, 13(3).

Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of Functional Foods, 18, 820-897.

Śliwa, P., & Śliwa, K. (2021). Nanomicellar extraction of polyphenols—methodology and applications review. International Journal of Molecular Sciences, 22(21), 11392.

Sorita, G. D., Leimann, F. V., & Ferreira, S. R. S. (2020). Biorefinery approach: is it an upgrade opportunity for peanut by-products? Trends in Food Science & Technology, 105, 56-69.

Sun, Z., Liang, P., Ding, Q., & Cao, J. (2006). Cloud point extraction preconcentration ofmanganese(II) from natural water samples using 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone and triton X-100 and determination by flame atomic absorptionspectrometry. Analytical Sciences, 22(6), 911–913.

Ulusoy, H. I., Yilmaz, Ö., & Gürkan, R. (2013). A micellar improved method for tracelevels selenium quantification in food samples, alcoholic and nonalcoholic beverages through CPE/FAAS. Food Chemistry, 139(1–4), 1008–1014.

Wang, W., Gao, Y. T., Wei, J. W., Chen, Y. F., Liu, Q. L., & Liu, H. M. (2021). Optimization of ultrasonic cellulase-assisted extraction and antioxidant activity of natural polyphenols from passion fruit. Molecules, 26(9), 2494.

Wee, J.-H., Moon, J.-H., Eun, J.-B., Chung, J.-H., Kim, Y.-G., Park, K.-H. (2007). Isolation and Identification of Antioxidants from Peanut Shells and the Relationship between Structure and Antioxidant Activity". Food Science and Biotechnology, 16(1), 116-122.

Win, M. M., Abdul-Hamid, A., Baharin, B. S., Anwar, F., Sabu, M. C., & Pak-Dek, M. S. (2011). Phenolic compounds and antioxidant activity of peanut’s skin, hull, raw kernel and roasted kernel flour. Pak. J. Bot, 43(3), 1635-1642.

Zhao, X., Chen, J., & Du, F. (2012). Potential use of peanut by-products in food processing: a review. Journal of Food Science and Technology, 49, 521-529.

Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559.

Zhou, J., Sun, J. B., Xu, X. Y., Cheng, Z. H., Zeng, P., Wang, F. Q., & Zhang, Q. (2015). Application of mixed cloud point extraction for the analysis of six flavonoids in Apocynum venetum leaf samples by high performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 107, 273-279.

Downloads

Published

11.01.2026

Issue

Section

Research Paper