Effects of Biochar Applications on the Growth and Bioactive Compound Content of Red Pansy (Viola wittrockiana)
DOI:
https://doi.org/10.24925/turjaf.v13i12.4252-4257.8427Keywords:
Antioxidant Activity , Individual Phenolic , Edible Flower , Biochar , YieldAbstract
The study examined the effects of biochar applications on the individual phenolic compounds, bioactive compound content, and yield of the edible flower Red Pansy (Viola Wittrockiana). The study was carried out in a greenhouse with a temperature of 18°C. A randomized plot design was used with three replications, with two pots per replicate. Two-liter pots were used in the study. Walnut shell and walnut pruning waste were used as the main biochar materials. Biochar was applied at a rate of 2%. Biochar applications caused an increase in some bioactive compounds and individual phenolics, while causing a decrease in others. To examine plant yield, the number of flowers, flower diameter, plant height, stem weight, root length, and root weight were determined. Significant increases were observed in all these parameters in both biochar applications compared to the control group. For example, the number of flowers recorded as 4.7 in the control group could be increased to 22.9 with biochar application. The data obtained from the study revealed that biochar is a good material for producing more productive Red Pansy flowers, but when the goal is to increase functional phytochemicals, characteristics such as application dose, biochar base material, and production temperature must be carefully selected.
References
Acir, Y., Erdem, H. (2020). “Biochar uygulamalarının ekmeklik buğdayın kadmiyum (Cd) alımına etkisi “, Akademik Ziraat Dergisi 9(2): 327-336 (2020) Araştırma ISSN: 2147-6403 e-ISSN: 2618-5881 DOI: http://dx.doi.org/10.29278/azd.813360, son erişim tarihi: 04.02.2022.
Akhtar, S. S., G. Li, M. N. Andersen, and F. Liu. (2014). Biochar enhances yield and quality of tomato under reduced irrigation. Agric. Water Manag.
Anteh, J.D., Almugrabi, E., Mostyakova, A., Timofeeva, O. (2023). Biochar influences onphytochemical composition and expression genes of curly kale at different treatmenttimes. Turk. J. Bot. 47 (6), 5. https://doi.org/10.55730/1300-008X.2782.
Benzie, İ.F.F. and Strain, J.J. (1996). The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay, Anal. Biochem., 239 (1), 70-76, 1996.
Beyhan, Ö., Elmastaş, M., & Gedikli, F. (2010). Total phenolic compounds and antioxidant capacity of leaf, dry fruit and fresh fruit of feijoa (Acca sellowiana, Myrtaceae). Journal of Medicinal Plant Research, 4, 1065-1072. https://doi.org/10.5897/JMPR10.008
Blois, M.S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature 1958, 181, 1199–1200.
Chen, X., Mao, X., Ding, Y., Chen. T., Wang, Y., Bao, J., Guo, L., Fang, L., Zhou, J. (2025). Biochar-induced microbial and metabolic reprogramming enhances bioactive compound accumulation in Panax quinquefolius L. BMC Plant Biol. 2025 May 20;25(1):669. doi: 10.1186/s12870-025-06656-x.
Conte, P., Marsala, V., De Pasquale, C., Bubici, S., Valagussa, M., Pozzi, A., Alonzo, G. (2013). “Nature of water-biochar interface interactions “, GCB Bioenergy 5:116–121.
Deng, R., Lan, Z., Shang, X., Fang, S. (2022). Effects of Biochar application pyrolyzed at different temperatures on soil properties, growth and leaf secondary metabolite accumulation in Cyclocarya paliurus. Forests 13:1572. https://doi.org/10.3390/f13101572.
El-Naggar, A., Lee, S.S., Rinklebe, J., Farooq, M., Song, H., Sarmah, A.K., Zimmerman, A.R., Ahmad, M., Shaheen, S.M., Ok, Y.S. (2019). Biochar application to low fertility soils: a review of current status, and future prospects. Geoderma, 337: 536-554.
Eo, J., Park, K.C., Kim, M.H., Kwon, S.I., Song, Y.J. (2018). “Effects of rice husk and rice husk biochar on root rot disease of ginseng (Panax ginseng) and on soil organisms “, Biological Agriculture & Horticulture, 34(1), 27-39.
Fernandes, L., Casal, S., Jose, A., Pereira, J.A., Saraiva, J.A. and Elsa Ramalhosa, E. (2020). An overview on the market of edible flowers. Food Reviews Internatıonal, 36, 258–275.
Githinji, A. (2014). “Effects of Training on Employee Performance: A Case Study of United Nations Support Office for the African Union Mission in Somalia “, (MBA Thesis) retrieved from http://erepo.usiu.ac.ke/handle/11732/71, son erişim tarihi: 04.02.2022.
Giusti, M.M., Wrolstad, R.E. (2001). Characterization and measurement of anthocyanins by UV-visible spectroscopy. In: Wrolstad, R.E, Editor. Current protocols in food analytical chemistry. John & Wiley, Inc. P F1.2.1- F1.2.13 New York, USA.
González-Barrio, R.; Periago, M.J.; Luna-Recio, C.; Garcia-Alonso, F.J.; Navarro-González, I. (2018). Chemical composition of the edible flowers, pansy (Viola wittrockiana) and snapdragon (Antirrhinum majus) as new sources of bioactive compounds. Food Chem. 2018, 252, 373–380.
Karhu, K., Mattila, T., Bergstrom, I., Regina, K. (2011). “Biochar addition to agri-cultural soil ıncreased ch4 uptake and water holding capacity results from a short-term pilot field study “, Agriculture, Ecosystems & Environment, 140: 309-313. doi.org/10.1016/ j. agee.2010.12.005.
Marchioni, I., Najar, B., Ruffoni, B., Copetta, A., & Pistelli, L. (2020). Bioactive compounds and aroma profile of some Lamiaceae edible flowers. Plants, 9(6), 691. https://doi.org/10.3390/plants9060691.
Oleszczuk, P., Rycaj, M., Lehmann, J., Cornelissen, G. (2012). “Influence of activated carbon and biochar on phytotoxicity of air-dried sewage sludges to Lepidium sativum “, Ecotoxicol. Environ, 80, 321–326.
Ozturk, B., Erdal, A., Karakaya, O., Saracoglu, O., Gün, S. (2019). Effects of preharvest GA3, CaCl2 and modified atmosphere packaging treatments on specific phenolic compounds of sweet cherry. Turk J. Food Agric. Sci.2019, 1 (2): 44-56
Regmi, A., Poudyal, S., Singh, S., Coldren, C., Moustaid-Moussa, N. (2023). Biochar Influences Phytochemical Concentrations of Viola cornuta Flowers. Sustainability 15: 3882. doi.org/10.3390/su15053882.
Ronsse, F., Van Hecke, S., Dickinson, D., & Prins, W. (2013). Production and characterization of slow pyrolysis biochar: influence of feedstock type and pyrolysis conditions. Gcb Bioenergy, 5(2), 104-115.
Rop, O., Mlcek, J., Jurikova, T., Neugebauerova, J. and Vabkova, J. (2012). Edible flowers—A New Promising Source of Mineral Elements in Human Nutrition. Molecules, 17, 6672–6683. Doi: 10.3390/molecules17066672
Sigua, G.C., Novak, J.M., Watts, D.W., Johnson M.G., Spokas, K. (2016). “Efficacies of designer biochars in improving biomass and nutrient uptake of winter wheat grown in a hard setting subsoil layer “, Chemosphere, 142, 176-183.
Sohi, S., Lopez-Capel, E., Krull, E., Bol, R. (2010). “Biochar climate change and soil: A review to guide future research “, Rep. No. 05/09. CSIRO.
Tag, A.T., Duman, G., Ucar, S., Yanik, J. (2016). “Effects of feedstock type and pyrolysis temperature on potential applications of biochar “, J. Anal Appl Pyrol 120:200–206.
Takahashi, J. A., Rezende, F., Moura, M., Dominguete, L., Sande, D. (2020). Edible flowers: Bioactive profile and its potential to be used in food development. Food Research International 129: 108868.
Yang, Z.Y. Ren, Z.T. Zhu, X.Z. Yang, W.Y. Ye, Z.Q. Tian, L.M. Ma, J.W. (2025). Biochar-based fertilizer increases soil nutrients and enhances tea quality: a metabolomics-based analysis. Front Plant Sci., 16 (2025), Article 1552759, 10.3389/fpls.2025.1552759.
Yılmaz-Vural, G., Erdem, H., Yıldız, K. (2025). "Optimizing Biochar Applications for Improved Growth and Nutritional Quality of Basil Plants Using Rice and Corn Biochars", Turkish Journal of Agriculture - Food Science and Technology. Vol. 13 No. 4.
Yuan, J., Xu, R.K., Zhang, H. (2011). “The forms of alkalis in the biochar produced from crop residues at different temperatures “, Bioresource Technology 102(3):3488-97.
Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
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