Antifungal Activity of Rosa damascena Leaf Extracts Against Botrytis cineria and Alternaria alternata
DOI:
https://doi.org/10.24925/turjaf.v13i12.4280-4285.8540Keywords:
Alternaria alternate, Antifungal activity, Botrytis cineria, R. damascena extractAbstract
This study was conducted to determine the in vitro antifungal activities of Rosa damascena ethanol, methanol, and hexane extracts against important plant pathogens, Botrytis cinerea and Alternaria alternata. In the study, the rates of inhibition of fungal mycelial growth by the extracts were determined on PDA medium at concentrations of 500, 1000, and 2000 µg/ml. In addition, to quantitatively evaluate antifungal activity, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) values (ranging from 125 to 4000 µg/ml using two-fold serial dilutions) were determined to assess the fungicidal effects of the extracts. The highest antifungal activity against both fungal phytopathogens was obtained from the hexane extract (B. cinerea 78.5% ± 3.0, A. alternata 84.1% ± 1.3). The MIC and MFC values of the extracts were determined to be 225–358 µg/ml and 2517–3960 µg/ml for B. cinerea, and 183–308 µg/ml and 2130–3917 µg/ml for A. alternata, respectively. These findings indicate that, particularly, the hexane extract may possess strong antifungal potential against economically important phytopathogens such as B. cinerea and A. alternata.
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