Synergistic Herbicide Applications Enhance Alfalfa (Medicago sativa L.) Yield, Weed Suppression and Forage Quality
DOI:
https://doi.org/10.24925/turjaf.v14i7.1881-1894.8762Keywords:
Alfalfa, Integrated weed management, Fodder and Forage, Livestock, Crude Protein (CP)Abstract
The livestock sector relies heavily on fodder crops, with alfalfa (Medicago sativa L.) being a prominent, high-nutritional-value legume in Southeast Asia. However, heavy weed infestation remains a major obstacle to quality production. This two-year study (2023–2024), evaluated the forage yield potential and nutritional quality of two alfalfa varieties (V1=Supersonic and V2=SGD-2002) under five weed control systems, including Weedy check (H0), Weed free (H1), Pendimethalin (H2), Flumetsulam (H3) and combination of both pre- and post-emergence application Pendimethalin + Flumetsulam (H4). Analysis of variance (ANOVA) across both seasons confirmed the highly significant effect of the herbicide factor on all yield and quality parameters, establishing weed control as the primary driver of productivity. The (H1) treatment consistently defined the biological maximum, achieving the highest fresh fodder yield (36.67 t ha-1) and superior quality (lowest Acid Detergent Fiber (ADF) and Neutral Detergent Fiber (NDF)). While, the (H0) resulted in severe yield loss, with minimum fresh fodder yield (12.15 t ha-1). Among the chemical applications, the (H4) was the most effective for production and suppression. This treatment consistently achieved the maximum fresh fodder yield (29.42 t ha-1) and the best weed control efficiency (up to 69%). However, the single application of (H2) performed best for nutritional quality, yielding maximum crude protein (20.33%) and lowest ADF among chemical controls. In conclusion, the (H4) combined herbicide strategy performed best overall, significantly suppressing weeds and maximizing crop productivity across both years, making it the most suitable chemical recommendation for high-yield alfalfa production.
References
Arshad, M. A., Abbas, R. N., Baloch, R., Ahmad, A., Zulfiqar, U., El-Beltagi, H. S., ... & Vara Prasad, P. V. (2025). Assessing herbicide efficacy and selectivity for weed management and enhancing the production of non-GMO soybean cultivation. Archives of Agronomy and Soil Science, 71(1), 1-23. https://doi.org/10.1080/03650340.2025.2554157
Bajwa, A. A. (2014). Sustainable weed management in conservation agriculture. Crop protection, 65, 105-113. https://doi.org/10.1016/j.cropro.2014.07.014
Behera, P., Das, N., & Karan, N. (2024). Sustainable weed management strategies for crop production. Comprehensive insights into environmental science. Stella International Publication, Kurukshetra, 159-180.
Bhattarai, S., Biswas, D., Fu, Y. B., & Biligetu, B. (2020). Morphological, physiological, and genetic responses to salt stress in alfalfa: A review. Agronomy, 10(4), 577. https://doi.org/10.3390/agronomy10040577
Bradley, K., Kallenbach, R., & Roberts, C. A. (2010). Influence of seeding rate and herbicide treatments on weed control, yield, and quality of spring‐seeded glyphosate‐resistant alfalfa. Agronomy Journal, 102(2), 751-758. https://doi.org/10.2134/agronj2009.0416
Chad Cummings, D., Berberet, R. C., Stritzke, J. F., & Caddel, J. L. (2004). Sod‐seeding and grazing effects on alfalfa weevils, weeds, and forage yields in established alfalfa. Agronomy Journal, 96(5), 1216-1221. https://doi.org/10.2134/agronj2004.1216
Chen, Y., Wang, H., Hu, W., Wang, S., Snider, J. L., & Zhou, Z. (2017). Co-occurring elevated temperature and waterlogging stresses disrupt cellulose synthesis by altering the expression and activity of carbohydrate balance-associated enzymes during fiber development in cotton. Environmental and Experimental Botany, 135, 106-117. https://doi.org/10.1016/j.envexpbot.2016.12.012
Choudhary, B. B., Sharma, P., Phand, S., Gupta, G., & Sharma, R. K. (2021). Agripreneurship development on value added fodder products [E-book]. Hyderabad: National Institute of Agricultural Extension Management & ICAR-Indian Grassland and Fodder Research Institute, Jhansi (UP). https://www.researchgate.net/profile/Bishwa-Choudhary/publication/360889417_Agripreneurship_Development_On_Value_Added_Fodder_Products/links/6290731cc660ab61f84880cf/Agripreneurship-Development-On-Value-Added-Fodder-Products.pdf
Dhakal, M., West, C. P., Villalobos, C., Brown, P., & Green, P. E. (2020). Inter-seeding alfalfa into native grassland for enhanced yield and water use efficiency. Agronomy Journal, 112(3), 1931-1942. https://doi.org/10.1002/agj2.20147
Dillehay, B. L., Curran, W. S., & Mortensen, D. A. (2011). Critical period for weed control in alfalfa. Weed science, 59(1), 68-75. https://doi.org/10.1614/WS-D-10-00073.1
El-Ramady, H., Abdalla, N., Kovacs, S., Domokos-Szabolcsy, É., Bákonyi, N., Fari, M., & Geilfus, C. M. (2020). Alfalfa growth under changing environments: An overview. Environment, Biodiversity and Soil Security, 4(2020), 201-224. https://doi.org/10.21608/jenvbs.2020.37746.1101
Fernandez, A. L., Fabrizzi, K. P., Tautges, N. E., Lamb, J. A., & Sheaffer, C. C. (2019). Cutting management and alfalfa stand age effects on organically grown corn grain yield and soil N availability. Renewable Agriculture and Food Systems, 34(2), 144-154. https://doi.org/10.1017/S1742170517000394
Food and Agriculture Organization Statistical Databases (FAOSTAT). 2023. Available online: https://www.fao.org/pakistan/our-office/pakistan-at-a-glance/en/. Accessed on 28-05-2024
Franco Teodoro Duarte, A. (2025). Addressing Management Barriers in Two Common Forage Systems in Wisconsin (Doctoral dissertation). https://minds.wisconsin.edu/handle/1793/95922
Gondal, M. R., Rizvi, S. A., Naseem, W., Khan, A., Hayat, S., Mustafa, S., Arif, M., Basit, A., & Salim, J. (2021). Role of weedicides for weed management and improvement in production of berseem (Trifolium alexandrinum L.) fodder. International Journal of Agricultural Technology, 17(4), 1329–1344. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6145
Govindasamy, P., Kantwa, S. R., Palsaniya, D. R., Ramakrishnan, S., Pooniya, V., Raj, R., & Saikia, N. (2025). Integrated weed management practices for effective weed control and quality fodder production in sorghum and cowpea under rainfed conditions. Range Management and Agroforestry, 46(1), 104-113. https://publications.rmsi.in/index.php/rma/article/view/1331
Hayat, M. K., Khan, A. R., Klutse, S., Rasool, M. W., & Mohsin, M. (2025). Empowering Crops: How Organic, Agronomic Practices and Technology Can Fortify Our Fields. World News of Natural Sciences, 59, 358-383. https://psjd.icm.edu.pl/psjd/element/bwmeta1.element.psjd-cf75060d-f9f3-4657-bb74-976c1e58d295/c/WNOFNS_59__2025__358-383.pdf
Hayat, M. K., Mumtaz, M. M., Ahsan Ali, M., Huaira, A., Ur Rehman, M., Shabbir, M. A., Usman, M., & Afzal, A. (2024). Optimizing Livestock Feed Systems: A Multi-Faceted Approach for Sustainable and Resilient Animal Agriculture; A Comprehensive Review. J. Plant Biota, 3, 27-33. https://doi.org/10.51470/JPB.2024.3.2.27
Hidosa, D., & Kibret, S. (2021). Dry matter yield and chemical composition of alfalfa (Medicago sativa) varieties as animal feed in the south Omo zone of South-western Ethiopia. Acta Scientific Veterinary Sciences (ISSN: 2582-3183), 3(4). https://www.researchgate.net/profile/DenbelaHidosa/publication/350721679_Dry_Matter_Yield_and_Chemical_Composition_of_Alfalfa_Medicago_sativa_Varieties_as_Animal_Feed_in_the_South_Omo_Zone_of_Southwestern_Ethiopia/links/606e9f83a6fdcc5f778cad5d/Dry-Matter-Yield-and-Chemical-Composition-of-Alfalfa-Medicago-sativa-Varieties-as-Animal-Feed-in-the-South-Omo-Zone-of-South-western-Ethiopia.pdf
Hopper, J. A., Peterson, H. H., & Burton Jr, R. O. (2004). Alfalfa hay quality and alternative pricing systems. Journal of agricultural and applied economics, 36(3), 675-690. https://doi.org/10.1017/S1074070800026948
Kanatas, P., Gazoulis, I., & Travlos, I. (2021). Irrigation timing as a practice of effective weed management in established alfalfa (Medicago sativa L.) crop. Agronomy, 11(3), 550. https://doi.org/10.3390/agronomy11030550
Kousta, A., Katsis, C., Tsekoura, A., & Chachalis, D. (2024). Effectiveness and selectivity of pre-and post-emergence herbicides for weed control in grain legumes. Plants, 13(2), 211. https://doi.org/10.3390/plants13020211
Kirilov, A., Georgieva, N., & Stoycheva, I. (2016). Determination of composition and palatability of certain weeds. International Journal of Agricultural Science and Food Technology, 2, 41-43. http://doi.org/10.17352/2455-815X.000013
Jalilian, A., Ghasemi, M. M., Ghaznavi, S., Moghadam, M. S. K., Mobtaker, H. G., Dastbala, G., & Kaab, A. (2025). Evaluating the effectiveness of Variable Rate Technology of herbicides and chemical fertilizers on reducing environmental pollutants and energy use in wheat production. Results in Engineering, 108722. https://doi.org/10.1016/j.rineng.2025.108722
Jeong, S. M., Lee, K. W., & Park, H. S. (2024). Effects of Chemical Foliar Herbicide on Weed Control in Alfalfa Field. Journal of The Korean Society of Grassland and Forage Science, 44(3), 140-144. https://doi.org/10.5333/KGFS.2024.44.3.140
Jehangir, I. A., Raja, W., Hussain, A., Al-Shuraym, L. A., Sayed, S. M., Lone, A. H., ... & Dar, E. A. (2024). Herbicide effectiveness and crop yield responses in direct-seeded rice: insights into sustainable weed management. Advances in Weed Science, 42, e020240004. https://doi.org/10.51694/AdvWeedSci/2024;42:00012
Latif, A., Sun, Y., & Noman, A. (2023). Herbaceous Alfalfa plant as a multipurpose crop and predominant forage specie in Pakistan. Frontiers in Sustainable Food Systems, 7, 1126151. https://doi.org/10.3389/fsufs.2023.1126151
Lee, B. H., Kim, J. H., Lee, K. W., Lee, S. Y., Jung, J. S., & Park, H. S. (2022). Effect of postemergence herbicides on dry matter yield and weed control in spring seeding alfalfa (Medicago sativa L.). https://doi.org/10.5333/KGFS.2022.42.1.10
Li, Q., Ren, Z., Xu, H., Wang, W., Zhang, Y., Huang, F., ... & Li, J. (2025). Sustainable Weed Management: The Effects of Applying Pre-and Post-Emergence Herbicides to Medicago ruthenica. Plants, 14(6), 864. https://doi.org/10.3390/plants14060864
Marten, G. C., Sheaffer, C. C., & Wyse, D. L. (1987). Forage nutritive value and palatability of perennial weeds. Agronomy Journal, 79(6), 980-986. https://doi.org/10.2134/agronj1987.00021962007900060006x
Matloob, A., Safdar, M. E., Abbas, T., Aslam, F., Khaliq, A., Tanveer, A., ... & Chadhar, A. R. (2020). Challenges and prospects for weed management in Pakistan: A review. Crop Protection, 134, 104724. https://doi.org/10.1016/j.cropro.2019.01.030
MacLaren, C., Storkey, J., Menegat, A., Metcalfe, H., & Dehnen-Schmutz, K. (2020). An ecological future for weed science to sustain crop production and the environment. A review: An ecological future for weed science to sustain crop production and the environment. A review. Agronomy for Sustainable Development, 40(4), 24. https://doi.org/10.1007/s13593-020-00631-6
Meighani, F., Mirvakili, S. M., Jahedi, A., Baghestani, M. A., & Shimi, P. (2010). Study of 2, 4-DB (Butress) efficacy in weed control in established alfalfa (Medicago sativa).
Milić, D., Katanski, S., Karagić, Đ., & Vasiljević, S. (2016). Alfalfa–the queen of forage crops: a guide for successful production. Novi Sad: Institut. za. Ratarstvo-i-povrtarstvo. https://fiver.ifvcns.rs/handle/123456789/2446
Mishra, M. M., Dash, R., & Mishra, M. (2016). Weed persistence, crop resistance and phytotonic effects of herbicides in direct-seeded rice. https://doi.org/10.5958/0974-8164.2016.00002.2
Mrabet, R. (2023). Sustainable agriculture for food and nutritional security. In Sustainable agriculture and the environment (pp. 25-90). Academic Press. https://doi.org/10.1016/B978-0-323-90500-8.00013-0
Noroozi, M., Dadashi, M. R., Maighany, F., & Ajamnorozei, H. (2023). Evaluation of forage yield and weed control of alfalfa (Medicago sativa L.) under the influence of different herbicides. https://doi.org/10.30495/IPER.2022.688800
Noroozi, M., Dadashi, M. R., Meighani, F., & Norouzi, H. A. (2022). Efficacy of different herbicides on weed control, growth indices and forage yield in alfalfa (Medicago sativa L.). Annali di Botanica, 12, 43-50. https://doi.org/10.13133/2239-3129/17623
Osterholz, W. R., Dias, J. L. C., Grabber, J. H., & Renz, M. J. (2021). PRE-and POST-applied herbicide options for alfalfa inter-seeded with corn silage. Weed Technology, 35(2), 263-270. https://doi.org/10.1017/wet.2020.104
Peng, W., Cai, W., Pan, J., Su, X., & Dou, L. (2025). Molecular Mechanisms of Alfalfa Response to Abiotic Stresses. Plants, 14(3), 487. https://doi.org/10.3390/plants14030487
Rana, S. S., & Rana, M. C. (2016). Principles and practices of weed management. Department of Agronomy, College of Agriculture, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur, 138.
Raoofi, M., & Alebrahim, M. T. (2017). A comparison of weeds interference and non-interference at different planting densities, on yield, nutritional value and some morphological traits of alfalfa (Medicago sativa L.). http://dx.doi.org/10.17582/journal.sja/2017/33.2.220.231
Razzaq, M. K., Aziz, A., Hayat, F., Ul Haq, M. Z., Rasool, I., Babur, M. N., ... & Hussain, K. (2025). Efficacy of Flumetsulam for Post-Emergence Weed Management in Chickpea (Cicer Arietinum L.) Under the Semi-Arid Region of Pakistan. Journal of Crop Health, 77(4), 111. https://doi.org/10.1007/s10343-025-01171-3
Shekhawat, K., Rathore, S. S., & Chauhan, B. S. (2020). Weed management in dry direct-seeded rice: A review on challenges and opportunities for sustainable rice production. Agronomy, 10(9), 1264. https://doi.org/10.3390/agronomy10091264
Shrotri, A., Kobayashi, H., & Fukuoka, A. (2017). Catalytic conversion of structural carbohydrates and lignin to chemicals. In Advances in catalysis (Vol. 60, pp. 59-123). Academic Press. https://doi.org/10.1016/bs.acat.2017.09.002
Steel, R. G. D., & Torrie, J. H. (1997). Boston, MA Principles and Procedures of Statistics. https://www.cabidigitallibrary.org/doi/full/10.5555/19611601129
Summers, H. (2020). Evaluating Integrated Weed Management Tactics to Reduce Herbicides and Control Herbicide-Resistant Weeds. https://etda.libraries.psu.edu/catalog/17418hns20
Tidemann, B. D., Harker, K. N., Shirtliffe, S., Willenborg, C., Johnson, E., Gulden, R., ... & Zuidhof, J. (2023). Using integrated weed management systems to manage herbicide-resistant weeds in the Canadian Prairies. Frontiers in Agronomy, 5, 1304741. https://doi.org/10.3389/fagro.2023.1304741
Viti, C., Bellabarba, A., Daghio, M., Mengoni, A., Mele, M., Buccioni, A., ... & Pini, F. (2021). Alfalfa for a sustainable ovine farming system: Proposed research for a new feeding strategy based on alfalfa and ecological leftovers in drought conditions. Sustainability, 13(7), 3880. https://doi.org/10.3390/su13073880
Wana, D., Abera, A., & Jane, W. (2024). Effect of intercropping oats (Avena sativa L.) With alfalfa (Medicago sativa) on dry matter yield, nutritive value, in vitro ruminal Fermentation kinetics and silage quality. https://repository.ju.edu.et//handle/123456789/9753
Wang, S. Y., Yang, G. L., Jing, Y. Y., He, Z. N., Liu, B., Sun, L., ... & Gao, F. (2025). Alfalfa quality improvement and loss reduction technology advances. Frontiers in Animal Science, 6, 1550492. https://doi.org/10.3389/fanim.2025.1550492
Wan, W., Li, Y., & Li, H. (2022). Yield and quality of alfalfa (Medicago sativa L.) in response to fertilizer application in China: A meta-analysis. Frontiers in plant science, 13, 1051725. https://doi.org/10.3389/fpls.2022.1051725
Wilson, R. G., & Burgener, P. A. (2009). Evaluation of glyphosate-tolerant and conventional alfalfa weed control systems during the first year of establishment. Weed Technology, 23(2), 257-263. https://doi.org/10.1614/WT-08-082.1
Zarzecka, K., Gugała, M., Mystkowska, I., & Sikorska, A. (2020). Total and true protein content in potato tubers depending on herbicides and biostimulants. Agronomy, 10(8), 1106. https://doi.org/10.3390/agronomy10081106
Zeller, F. M. E., Edmunds, B. L., & Schwarz, F. J. (2014). Effect of genotype on chemical composition, ruminal degradability and in vitro fermentation characteristics of maize residual plants. Journal of animal physiology and animal nutrition, 98(5), 982-990. https://doi.org/10.1111/jpn.12156
Zhang, Y., & Wang, L. (2025). Advances in basic biology of alfalfa (Medicago sativa L.): a comprehensive overview. Horticulture Research, 12(7), uhaf08. https://doi.org/10.1093/hr/uhaf081
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