Enzyme-Assisted Pectin Extraction from Pomegranate Peels
DOI:
https://doi.org/10.24925/turjaf.v14i7.1952-1962.8824Keywords:
Pectin, Pomegranate Peel, Enzymatic Extraction, Cellulase, OptimizationAbstract
In this study enzymatic extraction of pectin from pomegranate peel using cellulase was investigated. Extraction conditions were optimized by response surface methodology and the properties of the pectin obtained under optimum conditions were examined. The optimum enzyme level, substrate concentration, and extraction time were determined as 0.075 U/mL, 9.99 g pomegranate/100 mL, and 2.58 hours, respectively. A yield of 7.19% was obtained under these conditions, and the results of analysis revealed highly methoxylated pectin, whose structure was confirmed by FT-IR analysis. The results show that the enzyme-assisted extraction method can be successfully used instead of the hot acid extraction method for pectin isolation, and that pomegranate peel can be considered as an alternative pectin source for the production of highly methoxylated pectin.
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