Improvement of Salt Tolerance Potential of Solanum melongena through Backcrosses with Solanum linnaeanum

Esra Cebeci; Hatice Filiz Boyacı; Sevinç Kıran; Atilla Ata; Şeküre Şebnem Ellialtıoğlu

doi:10.24925/turjaf.v14i9.2453-2459.8871

Authors

Esra Cebeci Batı Akdeniz Agricultural Research Institute-Antalya, Türkiye https://orcid.org/0000-0003-0410-2453
Hatice Filiz Boyacı Recep Tayyip Erdogan University, Agricultural Faculty, Horticulture Department-Rize, Türkiye https://orcid.org/0000-0002-3799-4673
Sevinç Kıran Ankara University, Agricultural Faculty, Horticulture Department-Ankara, Türkiye https://orcid.org/0000-0001-6808-8326
Atilla Ata Alata Horticultural Research Institute-Mersin, Türkiye https://orcid.org/0000-0001-5479-5396
Şeküre Şebnem Ellialtıoğlu Ankara University, Doqutech Academy LLC Co.- Ankara, Türkiye https://orcid.org/0000-0002-3851-466X

DOI:

https://doi.org/10.24925/turjaf.v14i9.2453-2459.8871

Keywords:

Abiotic stress tolerance, Climate resilient breeding, Genetic introgression, Secondary gene pool , Wild relatives’ utilization

Abstract

Soil salinity is a major constraint on global agriculture, severely limiting the crop productivity. Eggplant (Solanum melongena L.), an economically important vegetable, is particularly sensitive to salt stress. Wild relatives such as Solanum linneanum L. provide valuable genetic resources for improving stress tolerance. This study evaluated the salt tolerance potential of S. linneanum and its backcross progenies with cultivated eggplant, aiming to identify breeding materials for the development of resilient cultivars. Three backcross lines (Lin1, Lin2, Lin3), derived from S. linneanum and the open-pollinated cultivar ‘Topan374’, were tested alongside ‘Topan374’ (negative control) and S. linneanum (positive control). The experiment followed a completely randomized block design with three replications, each consisting of six plants. Plants were subjected to 150 mM NaCl, and tolerance levels were assessed through morphological parameters (plant height, stem diameter, leaf number, anthocyanin presence and prickliness) and physiological stress indicators, including malondialdehyde (MDA) and proline content. Salt stress significantly reduced growth traits across genotypes. The wild parent maintained superior tolerance under 150 mM NaCl stress, while the backcross progenies (Lin1, Lin2, Lin3) showed better morphological performance than the sensitive cultivar, retaining higher growth under salinity. In addition, they exhibited elevated MDA and proline levels, in some cases exceeding those of the positive control, indicating activation of protective stress mechanisms. These findings demonstrate that introgression from S. linneanum can enhance salt tolerance in cultivated eggplant. The promising performance of backcross lines highlights their potential as valuable genetic resources for breeding salt-resilient cultivars, supporting sustainable eggplant production in saline-prone environments.

References

Akinci, I. E., Akinci, S., Yilmaz, K., & Dikici, H. (2004). Response of eggplant varieties (Solanum melongena) to salinity in germination and seedling stages. New Zealand Journal of Crop and Horticultural Science, 32(2), 193-200. https://doi.org/10.1080/01140671.2004.9514296

Bates. L. S.. Waldren. R. P.. & Teare. I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1), 205-207.

Benitez-Alfonso, Y., Soanes, B. K., Zimba, S., Sinanaj, B., German, L., Sharma, V., ... & Foyer, C. H. (2023). Enhancing climate change resilience in agricultural crops. Current Biology, 33(23), 1246-1261. https://doi.org/10.1016/j.cub.2023.10.028

Brenes, M., Pérez, J., González-Orenga, S., Solana, A., Boscaiu, M., Prohens, J., ... & Vicente, O. (2020a). Comparative studies on the physiological and biochemical responses to salt stress of eggplant (Solanum melongena) and its rootstock S. torvum. Agriculture, 10(8), 328. https://doi.org/10.3390/agriculture10080328

Brenes, M., Solana, A., Boscaiu, M., Fita, A., Vicente, O., Calatayud, Á., ... & Plazas, M. (2020b). Physiological and biochemical responses to salt stress in cultivated eggplant (Solanum melongena L.) and in S. insanum L., a close wild relative. Agronomy, 10(5), 651. https://doi.org/10.3390/agronomy10050651

Cebeci, E., Boyaci, H. F., Kiran, S., & Ellialtioglu, S. S. (2025a). Comprehensive assessment to reveal the salt tolerance potential of cultivated eggplants and their wild relatives. Frontiers in Plant Science, 16, 1483409. https://doi.org/10.3389/fpls.2025.1483409

Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş. (2025b). Screening of Eggplant F3 Segregating Population for Salt Tolerance. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 28(3), 746-756. https://doi.org/10.18016/ksutarimdoga.vi.1555674

Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş. (2024). Assessment results of salinity stressed f2 population originated from interspecific hybridization of eggplant with wild relative solanum incanum l. Horticultural Studies, 41(2), 50-59. https://doi.org/10.16882/hortis.1479101

Cebeci, E., Boyacı, H. F., Kıran, S., & Ellialtıoğlu, Ş. Ş. (2023). Responses of interspecific hybrid eggplant f4 inbred lines to drought and heat stress. Soil Studies, 12(2), 70-76. https://doi.org/10.21657/soilst.1408018

Daunay M.C. (2008): Eggplant. In: Prohens J., Nuez F. (eds): Handbook of Crop Breeding, Vegetables II: Fabaceae, Liliaceae, Umbelliferae, and Solanaceae. New York, Springer: 163−220.

Fang, Y., Wang, Z., Du, Y., Di, S., Gao, Z., Chen, X., ..& Li, Q. (2025). Comprehensive Evaluation and Screening for Salt Tolerance Germplasms at Seedling Stage in Eggplant. Horticulturae, 11(6), 697. https://doi.org/10.3390/horticulturae11060697

Gramazio, P., Alonso, D., Arrones, A., Villanueva, G., Plazas, M., Toppino, L., ... & Prohens, J. (2023). Conventional and new genetic resources for an eggplant breeding revolution. Journal of Experimental Botany, 74(20), 6285-6305. https://doi.org/10.1093/jxb/erad260

Gyanagoudar, H. S., Hatiya, S. T., Guhey, A., Dharmappa, P. M., & Seetharamaiah, S. K. (2024). A comprehensive approach for evaluating salinity stress tolerance in brinjal (Solanum melongena L.) germplasm using membership function value. Physiologia Plantarum, 176(2), e14239. https://doi.org/10.1111/ppl.14239

Hannachi, S., & Van Labeke, M. C. (2018). Salt stress affects germination, seedling growth and physiological responses differentially in eggplant cultivars (Solanum melongena L.). Scientia Horticulturae, 228, 56-65. https://doi.org/10.1016/j.scienta.2017.10.002

Hassani, A., Azapagic, A., & Shokri, N. (2021). Global predictions of primary soil salinization under changing climate in the 21st century. Nat Commun 12, 6663. https://doi.org/10.1038/s41467-021-26907-3

Harsha, S. G., Geetha, G. A., Manjugouda, I. P., Shilpashree, V. M., Singh, T. H., Laxman, R. H., ... & Shivashankara, K. S. (2025). Unraveling salinity stress tolerance: Contrasting morpho-physiological, biochemical, and ionic responses in diverse brinjal (Solanum melongena L.) genotypes. Plant Physiology and Biochemistry, 222, 109666. https://doi.org/10.1016/j.plaphy.2024.109666

Hazra, P., Chattopadhyay, A., & Hazra, S. (2025). Eggplant Genetic Resources. In Vegetable Crops (pp. 61-99). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-99-XXXX-X_3

Heuer, B., Meiri, A., Shalevet, J. (1986). Salt tolerance of eggplant. Plant Soil, 95, 9–13. doi: 10.1007/BF02378847. https://doi.org/10.1007/BF02378847

Hodges, D. M., DeLong, J. M., Forney, C. F., & Prange, R. K. (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta, 207(4), 604-611. https://doi.org/10.1007/s004250050524

Ismail, A., Takeda, S., & Nick, P. (2014). Life and death under salt stress: same players, different timing? Journal of Experimental Botany, 65(12), 2963-2979. https://doi.org/10.1093/jxb/eru159

Knapp, S., Vorontsova, M. S., & Prohens, J. (2013). Wild relatives of the eggplant (Solanum melongena L.: Solanaceae): new understanding of species names in a complex group. PloS one, 8(2), e57039. https://doi.org/10.1016/j.agwat.2024.109223

Kramer, I., Peleg, N., & Mau, Y. (2025). Climate change shifts risk of soil salinity and land degradation in water-scarce regions. Agricultural Water Management, 307, 109223. https://doi.org/10.1016/j.agwat.2024.109223

Kıran, S., Kuşvuran, Ş., Özkay, F., Özgün, Ö., Sönmez, K., Özbek, H., & Ellialtioğlu, Ş. Ş. (2015). Bazı patlıcan anaçlarının tuzluluk stresi koşullarındaki gelişmelerinin karşılaştırılması. International Journal of Agricultural and Natural Sciences, 8(1), 20-30.

Kopecká, R., Kameniarová, M., Černý, M., Brzobohatý, B., & Novák, J. (2023). Abiotic stress in crop production. International Journal of Molecular Sciences, 24(7), 6603. https://doi.org/10.3390/ijms24076603

Kouassi, B., Prohens, J., Gramazio, P., Kouassi, A. B., Vilanova, S., Galán-Ávila, A., ... & Plazas, M. (2016). Development of backcross generations and new interspecific hybrid combinations for introgression breeding in eggplant (Solanum melongena). Scientia Horticulturae, 213, 199-207. https://doi.org/10.1016/j.scienta.2016.10.033

Maas, E. V. (1990). “Crop,” in Agricultural Salinity Assessment and Management. Ed. Tanji, K. K. (New York: ASCE Publications), 262–304. ASCE Manuals and Reports on Engineering No. 71. https://doi.org/10.1061/9780872624322.ch09

Mace ES, Lester RN, Gebhardt CG (1999). AFLP analysis of genetic relationships among the cultivated eggplant, Solanum melongena L., and wild relatives (Solanaceae). Theoretical and Applied Genetics 99, 626–633. https://doi.org/10.1007/s001220051272

Mangino, G., Plazas, M., Vilanova, S., Prohens, J., & Gramazio, P. (2020). Performance of a set of eggplant (Solanum melongena) lines with introgressions from its wild relative S. incanum under open field and screenhouse conditions and detection of QTLs. Agronomy, 10(4), 467. https://doi.org/10.3390/agronomy10040467

Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59(1), 651-681. https://doi.org/10.1146/annurev.arplant.59.032607.092911

Mustafa, Z., Ayyub, C. M., Amjad, M., & Ahmad, R. (2017). Assessment of biochemical and ionic attributes against salt stress in eggplant (Solanum melongena L.) genotypes.

Oladosu, Y., Rafii, M. Y., Arolu, F., Chukwu, S. C., Salisu, M. A., Olaniyan, B. A., ... & Muftaudeen, T. K. (2021). Genetic diversity and utilization of cultivated eggplant germplasm in varietal improvement. Plants, 10(8), 1714. https://doi.org/10.3390/plants10081714

Ortega-Albero, N., González-Orenga, S., Vicente, O., Rodríguez-Burruezo, A., & Fita, A. (2023). Responses to salt stress of the interspecific hybrid Solanum insanum× Solanum melongena and its parental species. Plants, 12(2), 295. https://doi.org/10.3390/plants12020295

Özden, E., Tohumcu, F., & Sarı, S. (2025). Field-Based Assessment of Soil Salinity and Alkalinity Stress on Growth and Biochemical Responses in Eggplant (Solanum melongena L.). Agronomy, 15(8), 1945. https://doi.org/10.3390/agronomy15081945

Palmgren, M., & Shabala, S. (2024). Adapting crops for climate change: regaining lost abiotic stress tolerance in crops. Frontiers in Science, 2, 1416023. https://doi.org/10.3389/fsci.2024.1416023

Rahman, M. U., Zulfiqar, S., Raza, M. A., Ahmad, N., & Zhang, B. (2022). Engineering abiotic stress tolerance in crop plants through CRISPR genome editing. Cells, 11(22), 3590. https://doi.org/10.3390/cells11223590

Sanwal, S. K., Kumar, P., Kesh, H., Gupta, V. K., Kumar, A., Kumar, A., ... & Kumar, P. (2022). Salinity stress tolerance in potato cultivars: Evidence from physiological and biochemical traits. Plants, 11(14), 1842. https://doi.org/10.3390/plants11141842

Toppino, L., Barchi, L., & Rotino, G. L. (2022). Next generation breeding for abiotic stress resistance in eggplant. In genomic designing for abiotic stress resistant vegetable crops (pp. 115-151). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-92028-5_5

Unlukara, A., Kurunç, A., Kesmez, G. D., Yurtseven, E., Suarez, D. L. (2010). Effects of salinity on eggplant (Solanum melongena L.) growth and evapotranspiration. Irrig. Drain.: J. Int. Comm. Irrig. Drain. 59, 203–214. https://doi.org/10.1002/ird.453

Wang, W.; Vinocur, B.; Altman, A. (2003). Plant responses to drought, salinity and extreme temperatures: Towards genetic engineering for stress tolerance. Planta, 218, 1-14. https://doi.org/10.1007/s00425-003-1105-5

Yang, Y.; Guo, Y. (2018). Elucidating the molecular mechanisms mediating plant salt-stress responses. New Phytolojist, 217, 523–539. https://doi.org/10.1111/nph.14920

Yaşar, F., & Ellialtıoğlu, Ş. (2013). Antioxidative responses of some eggplant genotypes to salinity stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 23(3), 215-221.

Zhang, H., Lang, Z., Zhu, J. K., & Wang, P. (2025). Tackling abiotic stress in plants: recent insights and trends. Stress Biology, 5(1), 8. https://doi.org/10.1007/s44154-025-00080-7

Zittis, G., Almazroui, M., Alpert, P., Ciais, P., Cramer, W., Dahdal, Y., ... & Lelieveld, J. (2022). Climate change and weather extremes in the Eastern Mediterranean and Middle East. Reviews of Geophysics, 60(3), e2021RG000762. https://doi.org/10.1029/2021RG000762