Dietary factors affecting milk fat synthesis and composition in dairy cows

Authors

DOI:

https://doi.org/10.24925/turjaf.v14i9.2718-2724.9347

Keywords:

Dairy nutrition, Diet, Fatty acids, Lipid supplementation, Milk fat

Abstract

In recent years, there has been a trend towards increasing and/or maintaining the milk fat content in dairy production. The main reasons for this include the fact that milk pricing is based on milk fat content, the increased demand for dairy products such as cheese and butter, and the obligation to maintain milk fat at certain levels to ensure profitability in dairy farming. Milk fat is the most variable component of milk and can be influenced by numerous nutritional and non-nutritional factors. The nutritional factors affecting milk fat content can be listed primarily as the forage to concentrate ratio, the balance between dietary starch and fiber, the level and type of lipid supplementation, the type of dietary forage, access to pasture, ruminal biohydrogenation, and dietary amino acid composition. Non-nutritional factors affecting milk fat composition include genetics, lactation stage, parity, season, health, stress, breed and management. The aim of this review is to identify the nutritional factors affecting milk fat in dairy cattle, to explain and discuss current/novel nutritional strategies, to clarify the effects of certain dietary manipulations on milk fat in the light of literature, and to facilitate an understanding of the mechanisms of milk fat production.

References

Adeniji, Y. A., Matamoros, C., Walker, R. E., & Harvatine, K. J. (2025). Relationship between oxygenated fatty acid and milk fat concentration during diet-induced milk fat depression in dairy cows. JDS Communications, 6(6), 761-765. https://doi.org/10.3168/jdsc.2025-0812

Akdağ, Ş., Akdağ, A., & Garipoğlu, A. (2026). Comprehending the Significance of Physically Effective Neutral Detergent Fibre in Ruminant Nutrition. Black Sea Journal of Agriculture, 9(3), 469-476. https://doi.org/10.47115/bsagriculture.1883317

Angeles-Hernandez, J. C., Alberto, R. V., Kebreab, E., Appuhamy, J. A. R. N., Dougherty, H. C., Castelan-Ortega, O., & Gonzalez-Ronquillo, M. (2020). Effect of forage to concentrate ratio and fat supplementation on milk composition in dairy sheep: A meta-analysis. Livestock Science, 238, 104069. https://doi.org/10.1016/j.livsci.2020.104069

Bauman, D. E., & Griinari, J. M. (2003). Nutritional regulation of milk fat synthesis. Annual Review of Nutrition, 23:203–227. https://doi.org/10.1146/annurev.nutr.23.011702.073408.

Baumgard, L. H., Matitashvili, E., Corl, B. A., Dwyer, D. A., & Bauman, D. E. (2002). Trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. Journal of Dairy Science, 85(9), 2155-2163. https://doi.org/10.3168/jds.S0022-0302(02)74294-X

Beauchemin, K. A. (2018). Invited review: Current perspectives on eating and rumination activity in dairy cows. Journal of Dairy Science, 101(6), 4762-4784. https://doi.org/10.3168/jds.2017-13706

Broderick, G. A., & Radloff, W. J. (2004). Effect of molasses supplementation on the production of lactating dairy cows fed diets based on alfalfa and corn silage. Journal of Dairy Science, 87(9), 2997-3009. https://doi.org/10.3168/jds.S0022-0302(04)73431-1

Chaucheyras-Durand, F., Walker, N. D., & Bach, A. (2008). Effects of active dry yeasts on the rumen microbial ecosystem: Past, present and future. Animal Feed Science and Technology, 145(1-4), 5-26. https://doi.org/10.1016/j.anifeedsci.2007.04.019

Che, L., Xu, M., Gao, K., Zhu, C., Wang, L., Yang, X., Wen, X., Xiao, H., Jiang, Z., & D. Wu. (2019). Valine increases milk fat synthesis in mammary gland of gilts through stimulating AKT/MTOR/SREBP1 pathway. Biology of Reproduction, 101:126–137. https://doi.org/10.1093/biolre/ioz065.

Chen, B., Wang, C., Wang, Y. M., & Liu, J. X. (2011). Effect of biotin on milk performance of dairy cattle: A meta-analysis. Journal of Dairy Science, 94(7), 3537-3546. https://doi.org/10.3168/jds.2010-3764

Chilliard, Y., Ferlay, A., Mansbridge, R. M., & Doreau, M. (2000). Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. In Annales de zootechnie. EDP Sciences, 49 (3), 181-205.

Daley, V. L., Armentano, L. E., & Hanigan, M. D. (2022). Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis. Journal of Dairy Science, 105(10), 8016-8035. https://doi.org/10.3168/jds.2022-21777

Davis, H., Chatzidimitriou, E., Leifert, C., & Butler, G. (2020). Evidence that forage-fed cows can enhance milk quality. Sustainability, 12(9), 3688. https://doi.org/10.3390/su12093688

Dewanckele, L., Toral, P. G., Vlaeminck, B., & Fievez, V. (2020). Invited review: Role of rumen biohydrogenation intermediates and rumen microbes in diet-induced milk fat depression: An update. Journal of Dairy Science, 103(9), 7655-7681. https://doi.org/10.3168/jds.2019-17662

Dhillon, K. S., & Pal, R. P. (2025). Effect of feeding total mixed ration supplemented with sodium bicarbonate and magnesium oxide on milk yield, milk composition and manure score in early lactating dairy cattle. Indian Journal of Dairy Science, 78(3), 271-277. https://doi.org/ 10.33785/IJDS. 2025.v78i03.011

Eastridge, M. L., (2020). Sugar in Diets for Lactating Dairy Cows. Accessed 14 July, 2026. https://www.dairyherd.com/news/sugar-diets-lactating-dairy-cows

Golder, H. M., & Lean, I. J. (2024). Invited review: Ruminal acidosis and its definition—A critical review. Journal of Dairy Science, 107(12), 10066-10098. https://doi.org/10.3168/jds.2024-24817

Graulet, B., Matte, J. J., Desrochers, A., Doepel, L., Palin, M. F., & Girard, C. L. (2007). Effects of dietary supplements of folic acid and vitamin B12 on metabolism of dairy cows in early lactation. Journal of Dairy Science, 90(7), 3442-3455. https://doi.org/10.3168/jds.2006-718

Guo, Y., Wei, Z., Zhang, Y., & Cao, J. (2024). Research progress on the mechanism of milk fat synthesis in cows and the effect of conjugated linoleic acid on milk fat metabolism and its underlying mechanism: A Review. Animals, 14(2), 204. https://doi.org/10.3390/ani14020204

Hanuš, O., Samková, E., Křížová, L., Hasoňová, L., & Kala, R. (2018). Role of fatty acids in milk fat and the influence of selected factors on their variability—a review. Molecules, 23(7), 1636. https://doi.org/10.3390/molecules23071636

Harrison, J., White, R., Kincaid, R., Block, E., Jenkins, T., & St-Pierre, N. (2012). Effectiveness of potassium carbonate sesquihydrate to increase dietary cation-anion difference in early lactation cows. Journal of Dairy Science, 95(7), 3919-3925. https://doi.org/10.3168/jds.2011-4840

He, M., & Armentano, L. E. (2011). Effect of fatty acid profile in vegetable oils and antioxidant supplementation on dairy cattle performance and milk fat depression. Journal of dairy science, 94(5), 2481-2491. https://doi.org/10.3168/jds.2010-3755

Heffernan, C., O'Callaghan, T. F., Fitzgerald, R., Serra, E., Frizzarin, M., Galvin, N., & Dineen, M. (2025). Investigating the effects of concentrate supplement level and type on milk fat production and animal performance of spring-calving grazing dairy cows. Journal of Dairy Science, 108(6), 5968-5981. https://doi.org/10.3168/jds.2024-25928

Iwaniuk, M. E., & Erdman, R. A. (2015). Intake, milk production, ruminal, and feed efficiency responses to dietary cation-anion difference by lactating dairy cows. Journal of Dairy Science, 98(12), 8973-8985. https://doi.org/10.3168/jds.2015-9949

Jaakamo, M. J., Luukkonen, T. J., Kairenius, P. K., Bayat, A. R., Ahvenjärvi, S. A., Vilkki, J. H., & Leskinen, H. M. (2024). Effects of dietary forage-to-concentrate ratio and forage type on milk phospholipids and fatty acid composition of polar lipids. Journal of Dairy Science, 107(3), 1450-1459. https://doi.org/10.3168/jds.2023-23842

Jenkins, T. C., & McGuire, M. A. (2006). Major advances in nutrition: impact on milk composition. Journal of Dairy Science, 89(4), 1302-1310. https://doi.org/10.3168/jds.S0022-0302(06)72198-1

Jenkins, T. C., Bridges Jr, W. C., Harrison, J. H., & Young, K. M. (2014). Addition of potassium carbonate to continuous cultures of mixed ruminal bacteria shifts volatile fatty acids and daily production of biohydrogenation intermediates. Journal of Dairy Science, 97(2), 975-984. https://doi.org/10.3168/jds.2013-7164

Kalač, P., & Samková, E. J. C. J. (2010). The effects of feeding various forages on fatty acid composition of bovine milk fat: A review. Czech Journal of Animal Science, 55(12), 521-537. https://doi.org/ 10.17221/2485-CJAS

Kalscheur, K. F., Teter, B. B., Piperova, L. S., & Erdman, R. A. (1997). Effect of dietary forage concentration and buffer addition on duodenal flow of trans-C18: 1 fatty acids and milk fat production in dairy cows. Journal of Dairy Science, 80(9), 2104-2114.

Krajcarski-Hunt, H., Plaizier, J. C., Walton, J. P., Spratt, R., & McBride, B. W. (2002). Effect of subacute ruminal acidosis on in situ fiber digestion in lactating dairy cows. Journal of Dairy Science, 85(3), 570-573.

Kröger, I., Humer, E., Neubauer, V., Reisinger, N., & Zebeli, Q. (2019). Feeding diets moderate in physically effective fibre alters eating and feed sorting patterns without improving ruminal pH, but impaired liver health in dairy cows. Animals, 9(4), 128. https://doi.org/10.3390/ani9040128

Leroux, C., Le Provost, F., Petit, E., Bernard, L., Chilliard, Y., & Martin, P. (2003). Real-time RT-PCR and cDNA macroarray to study the impact of the genetic polymorphism at the $alpha_ {s1} $-casein locus on the expression of genes in the goat mammary gland during lactation. Reproduction Nutrition Development, 43(5), 459-469.

Lin, M., Jiang, M., Yang, T., Tan, D., Hu, G., Zhao, G., & Zhan, K. (2022). Acetate-induced milk fat synthesis is associated with activation of the mTOR signaling pathway in bovine mammary epithelial cells. Animals, 12(19), 2616. https://doi.org/10.3390/ani12192616

MacGibbon, A., & Taylor, M. (2006). Composition and Structure of Bovine Milk Lipids. In Advanced Dairy Chemistry; Fox, P., McSweeney, P., Eds.; Springer: New York, NY, USA, pp. 1–42.

Mertens, D. R. (1997). Creating a system for meeting the fiber requirements of dairy cows. Journal of Dairy Science, 80(7), 1463-1481.

Mikołajczyk, K., Pecka-Kiełb, E., & Zachwieja, A. (2019). Impact of the volume and the profile of volatile fatty acids in the rumen fermentation on cow productivity and milk composition. Dairy/Mljekarstvo, 69(4). https://doi.org/10.15567/mljeKarstvo.2019.0402

Moussiaux, I. G., Vandevoorde, T., Robinson, M., & Krogstad, K. C. (2026). Evaluating dietary starch concentrations in reduced-forage rations fed to lactating dairy cattle. Journal of Dairy Science. https://doi.org/10.3168/jds.2026-28323

Mu, T., Hu, H., Ma, Y., Feng, X., Zhang, J., & Gu, Y. (2021). Regulation of key genes for milk fat synthesis in ruminants. Frontiers in Nutrition, 8, 765147. https://doi.org/10.3389/fnut.2021.765147

NASEM, National Academies of Sciences, Engineering, and Medicine. (2021). Title of the specific report goes here. The National Academies Press.

Neville, M.C., & Picciano, M.F. (1997). Regulation of Milk Lipid Secretion and Composition. Annual Review of Nutrition, 17, 159–184.

Olijhoek, D. W., Løvendahl, P., Lassen, J., Hellwing, A. L. F., Höglund, J. K., Weisbjerg, M. R., & Lund, P. (2018). Methane production, rumen fermentation, and diet digestibility of Holstein and Jersey dairy cows being divergent in residual feed intake and fed at 2 forage-to-concentrate ratios. Journal of Dairy Science, 101(11), 9926-9940. https://doi.org/10.3168/jds.2017-14278

Osorio, J.S., Lohakare, J., Bionaz, M. (2016). Biosynthesis of milk fat, protein, and lactose: Roles of transcriptional and posttranscriptional regulation. Physiological Genomics, 48, 231–256. https://doi.org/10.1152/physiolgenomics.00016.2015

Pacheco-Pappenheim, S., Yener, S., Nichols, K., Dijkstra, J., Hettinga, K., & van Valenberg, H. J. (2022). Feeding hydrogenated palm fatty acids and rumen-protected protein to lactating Holstein-Friesian dairy cows modifies milk fat triacylglycerol composition and structure, and solid fat content. Journal of Dairy Science, 105(4), 2828-2839. https://doi.org/10.3168/jds.2021-21083

Palmquist, D. L., Beaulieu, A. D., & Barbano, D. M. (1993). Feed and animal factors influencing milk fat composition. Journal of Dairy Science, 76(6), 1753-1771.

Piperova, L. S., Sampugna, J., Teter, B. B., Kalscheur, K. F., Yurawecz, M. P., Ku, Y., ... & Erdman, R. A. (2002). Duodenal and milk trans octadecenoic acid and conjugated linoleic acid (CLA) isomers indicate that postabsorptive synthesis is the predominant source of cis-9-containing CLA in lactating dairy cows. The Journal of Nutrition, 132(6), 1235-1241.

Plaizier, J. C., Krause, D. O., Gozho, G. N., & McBride, B. W. (2008). Subacute ruminal acidosis in dairy cows: The physiological causes, incidence and consequences. The Veterinary Journal, 176(1), 21-31. https://doi.org/10.1016/j.tvjl.2007.12.016

Rico, D. E., & Razzaghi, A. (2023). Animal board invited review: The contribution of adipose stores to milk fat: Implications on optimal nutritional strategies to increase milk fat synthesis in dairy cows. Animal, 17(4), 100735. https://doi.org/10.1016/j.animal.2023.100735

Rodríguez-Bermúdez, R., Fouz, R., Rico, M., Camino, F., Souza, T.K., Miranda, M., Diéguez, F.J. Factors Affecting Fatty Acid Composition of Holstein Cow’s Milk. Animals 2023, 13, 574. https://doi.org/10.3390/ani13040574

Rojas-Downing, M. M., Nejadhashemi, A. P., Harrigan, T., & Woznicki, S. A. (2017). Climate change and livestock: Impacts, adaptation, and mitigation. In Climate Risk Management (Vol. 16, pp. 145–163). Elsevier B.V. https://doi.org/10.1016/j.crm.2017.02.001

Salfer, I. J., Matamoros, C. I., Bartell, P. A., & Harvatine, K. J. (2023). Effects of the timing of protein infusion on the daily rhythms of milk synthesis and plasma hormones and metabolites in dairy cows. Journal of Dairy Science, 106(8), 5351-5363. https://doi.org/10.3168/jds.2022-22633

Samková, E., Špička, J., Pešek, M., Pelikánová, T., & Hanuš, O. (2012). Animal factors affecting fatty acid composition of cow milk fat: A review. South African Journal of Animal Science, 42(2), 83-100. http://dx.doi.org/10.4314/sajas.v42i2.1

Sanh, M. V., Wiktorsson, H., & Ly, L. V. (2002). Effects of natural grass forage to concentrate ratios and feeding principles on milk production and performance of crossbred lactating cows. Asian-australasian journal of animal sciences, 15(5), 650-657.

Schroeder, G. F., Gagliostro, G. A., Bargo, F., Delahoy, J. E., & Muller, L. D. (2004). Effects of fat supplementation on milk production and composition by dairy cows on pasture: a review. Livestock Production Science, 86(1-3), 1-18.

Urrutia, N. L., Muñoz, C., Ungerfeld, E. M., Cisterna, C., & Harvatine, K. J. (2025). Interaction between ruminal acetate infusion and diet fermentability on milk fat production in dairy cows. Animals, 15(13), 1931. https://doi.org/10.3390/ani15131931

USDA. 2021. Farm milk components and their use among dairy products have shifted over time. Accessed June 28, 2026. https://www.ers.usda.gov/amber-waves/2021/ august/ farm -milk -components-and -their -use -among -dairy -products -have -shifted -over -time/.

Vahmani, P., Xu, Y., Dugan, M. E., & Hackmann, T. J. (2024). Trans-10 shifted ruminal biohydrogenation and its implications for ruminant milk and meat fat content and quality. Canadian Journal of Animal Science, 105, 1-10. https://doi.org/10.1139/cjas-2024-0028

Van Amburgh, M. E., & Benoit, A. C. (2025). Balancing dietary amino acids for optimum milk fat synthesis and implications of dietary fatty acid supply and profile–a modeling exercise.

Vitt, M. G. D., Brunetto, A. L. R., Leal, K. W., Deolindo, G. L., Corrêa, N. G., Silva, L. E. L. E., & Silva, A. S. D. (2025). Use of a Blend of Exogenous Enzymes in the Diet of Lactating Jersey Cows: Ruminal Fermentation In Vivo and In Vitro, and Its Effects on Productive Performance, Milk Quality, and Animal Health. Fermentation, 11(9), 495. https://doi.org/10.3390/fermentation11090495

Walker, G. P., Dunshea, F. R., & Doyle, P. T. (2004). Effects of nutrition and management on the production and composition of milk fat and protein: a review. Australian Journal of Agricultural Research, 55(10), 1009-1028.

Wei, X. S., Cai, C. J., He, J. J., Yu, C., Mitloehner, F., Liu, B. L., ... & Cao, Y. C. (2018). Effects of biotin and nicotinamide supplementation on glucose and lipid metabolism and milk production of transition dairy cows. Animal Feed Science and Technology, 237, 106-117. https://doi.org/10.1016/j.anifeedsci.2018.01.012

Wiking, L., Stagsted, J., Bjorck, L., & Nielsen, J. H. (2004). Milk fat globule size is affected by fat production in dairy cows. International Dairy Journal, 14, 909–913. https://doi.org/10.1016/j.idairyj.2004.03.005.

Yu, Y., Fu, R., Jin, C., Gao, H., Han, L., Fu, B., & Leng, J. (2024). Regulation of milk fat synthesis: key genes and microbial functions. Microorganisms, 12(11), 2302. https://doi.org/10.3390/microorganisms12112302

Zhao, Y., Guo, X., Yan, S., Shi, B., & Sheng, R. (2021). Acetate regulates milk fat synthesis through the mammalian target of rapamycin/eukaryotic initiation factor 4E signaling pathway in bovine mammary epithelial cells. Journal of Dairy Science, 104(1), 337-345. https://doi.org/10.3168/jds.2020-18246

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17.09.2026

How to Cite

Akdağ, A. (2026). Dietary factors affecting milk fat synthesis and composition in dairy cows. Turkish Journal of Agriculture - Food Science and Technology, 14(9), 2738–2744. https://doi.org/10.24925/turjaf.v14i9.2718-2724.9347

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Review Articles