PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments

Authors

  • Homaira Anzum Himi Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,
  • Md. Shafiullah Parvej Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202,
  • M. Bahanur Rahman
  • K M Nasiruddin Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,
  • Wahedul Karim Ansari Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,
  • Md. Mostakin Ahamed Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,

DOI:

https://doi.org/10.24925/turjaf.v3i6.361-364.293

Keywords:

Commercial Poultry, Ciprofloxacin, E. coli, Shiga toxin, PCR

Abstract

Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram‘s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin.

Author Biography

M. Bahanur Rahman

Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202,

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Published

28.02.2015

How to Cite

Himi, H. A., Parvej, M. S., Rahman, M. B., Nasiruddin, K. M., Ansari, W. K., & Ahamed, M. M. (2015). PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments. Turkish Journal of Agriculture - Food Science and Technology, 3(6), 361–364. https://doi.org/10.24925/turjaf.v3i6.361-364.293

Issue

Section

Animal Production