Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Authors

  • Mehdi Ghorbani Master Degree in Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Iran.
  • Aghil Ghorbani M.Sc. students of Agricultural Biotechnology, Department of Crop Biotechnology and Breeding, Ferdowsi University of Mashhad, Iran.
  • Mansoor Omidi Professor of Molecular Genetics and Biotechnology, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Iran.
  • Seyed Mohammad Hashemi M.Sc. students of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Iran.

DOI:

https://doi.org/10.24925/turjaf.v3i6.349-443.320

Keywords:

Agrobacterium rhizogenes, Hairy root culture, Noradrenaline, Portulaca oleracea L., Response surface methodology

Abstract

Common purslane (Portulaca oleracea L.) is an annual plant as one of the natural sources for noradrenaline hormone. In this research, hairy root culture of purslane was established by using Agrobacterium rhizogenes strain ATCC 15834. In the following, Box-Behnken model of response surface methodology (RSM) was employed to optimize B5 medium for the growth of P. oleracea L. hairy root line. According to the results, modelling and optimization conditions, including sucrose, CaCl2.H2O, H2PO4 and NO3-/NH4+ concentrations on maximum dry weight (0.155 g) and noradrenaline content (0.36 mg.g-1 DW) was predicted. These optimal conditions predicted by RSM were confirmed the enhancement of noradrenaline production as an application potential for production by hairy root cultures.

Author Biographies

Aghil Ghorbani, M.Sc. students of Agricultural Biotechnology, Department of Crop Biotechnology and Breeding, Ferdowsi University of Mashhad, Iran.

Ferdowsi University of Mashhad

Mansoor Omidi, Professor of Molecular Genetics and Biotechnology, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Iran.

University of Tehran

Seyed Mohammad Hashemi, M.Sc. students of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Iran.

University of Tehran

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Published

25.03.2015

How to Cite

Ghorbani, M., Ghorbani, A., Omidi, M., & Hashemi, S. M. (2015). Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.). Turkish Journal of Agriculture - Food Science and Technology, 3(6), 349–443. https://doi.org/10.24925/turjaf.v3i6.349-443.320

Issue

Vol. 3 No. 6 (2015)

Section

Agricultural Technologies

License

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.