Impact of Extraction Solvent Polarity: Antioxydant Activity of Methanolic, Hydromethanolic and Aqueous Decocted Extracts of Algerien Thymelaea hirsuta (L.) Endl. Areal Parts
Keywords:Thymelaea hirsuta, antioxidant, solvent polarity, reducing power.
Thymelaea hirsuta (L.) Endl. , known as ‘Methnane’ in Algeria, is a widely medicinal plant used in folk medicine. In the present study, In vitro antioxidant activity of T. hirsta extracts and the impact of extraction solvent polarity on the antioxidant potential were investigated. Three types of polar solvents with decreasing polarity were chosen; water for decocted extract, methanol-water at 50% and absolute methanol for macerated extracts. Total phenolic and flavonoid contents were evaluated and showed a high amount wihch decreases with increasing polarity. Antioxydant activity was assessed with different methods: ABTS assay for evaluation of scavenge activity, CUPRAC and reducing power for assessement of the reduction potential of T. hirsuta areal parts. The results showed that T. hirsuta areal parts exhibited a strong scavenging activity with significant difference between extracts in terms of their polarity. In the same line, the most polar aqueous decocted extract exhibited a considerable reducing activity followed by hydromethanolic and methanolic extract with increasing potential. These findings suggest the suitability of polar solvents for the extraction of phytochemical compounds from T. hirsuta areal parts and so, their antioxidant activity against several radicals and ions.
Adam G, Raffaella Mammucari, Foster NR. 2011. A review of subcritical water as a solvent and its utilisation for the processing of hydrophobic organic compounds, Chemical Engineering Journal, Volume 172, Issue 1, 2011, Pages 1-17, ISSN 1385-8947, https://doi.org/10.1016/j.cej.06.007.
Akiyama H, Sakushima J, Taniuchi S, Kanda T, Yanagida A, Kojima T, Teshima R, Kobayashi Y, Goda Y, Toyoda M. 2000. Antiallergic effect of apple polyphenols on the allergic model mouse. Biological and Pharmaceutical Bulletin; l (23): 1370. https://doi.org/10.1248/bpb.23.1370
Amari N, Bouzouina, M, Berkani A, Lotmani B. 2014. Phytochemical screening and antioxidant capacity of the aerial parts of Thymelaea hirsuta L. Asian Pacific journal of tropical disease; 4(2): 104-109, https://doi.org/10.1016/S2222-1808(14)60324-8
Apak R, Guclu K, Ozyurek M, Karademir SE. 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry; 52: 7970-7981, https://doi.org/10.1021/jf048741x
Owen B B, Miller R C, Milner C E, Cogan H L. 1961. The Dielectric Constant of Water as a Function of Temperature and Pressure. The Journal of Physical Chemistry; 65: 2065- 2070.
Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, Pinkas M. 1996. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittel-forschung; 46(11): 1086-1089.
Benchikh F, Amira S, Benabdallah H. 2018. The evaluation of antioxidant capacity of different fractions of Myrtus communis L. leaves. Annual Research & Review in Biology, 22(5): 1-14. DOI:10.9734/ARRB/2018/39217
Ben Farhat M, Chaouch-Hamada R, Sotomayor JA, Landoulsi A, Jordán MJ. 2015. Antioxidant properties and evaluation of phytochemical composition of Salvia verbenaca L. extracts at different developmental stages. Plant Foods for Human Nutrition; 70(1): 15-20. https://doi.org/10.1007/s1113 0-015-0466-9
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature; 18: 1199-1200
Borris RP, Blask OG, Cordell GA. 1988. Ethnopharmacologic and phytochemical studies of the Thymelaeaceae. Journal of Ethnopharmacology; 24: 41-91, https://doi.org/10.1016/0378-8741(88)90138-9
Castilla P, Echarri R, Dávalos A, Cerrato F, Ortega H, Luis Teruel J, Fernández Lucas M, Gómez-Coronado L, Ortuño J, Lasunción MA. 2006. Concentrated red grape juice exerts antioxidant, hypolipidemic, and antiinflammatory effects in both hemodialysis patients and healthy subjects, The American Journal of Clinical Nutrition; 84-252. https://doi.org/10.1093/ajcn/84.1.252
Djermane N, Gali L, Arhab R, Gherraf N, Bensouici C, Erenler R, Gok M, Abdessamed A. 2020. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, and enzyme inhibitory activities of Erucaria uncata and Thymeleae hirsuta. Biocatalysis and Agricultural Biotechnology, https://doi.org/10.1016/j.bcab.2020.101834.
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis; 22: 296-302 doi: 10.1016/j.jfda.2013.11.001.
Ferreira A, Proença C, Serralheiro ML, and Araújo ME. 2006. The in vitro screening for acetyl cholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. Journal of Ethnopharmacology; 108: 31-37, https://doi.org/10.1016/j.jep.2006.04.010
Friedman J, Yaniv Z, Dafni A, Palewitch D. 1986. A preliminary classification of the of the healing potential of medicinal plants, based on a rational analysis of an ethnopharmacological field survey among Bedouins in the Negev Desert, Israel. Journal of ethnopharmacology; 16(2-3): 275-287, https://doi.org/10.1016/0378-8741(86)90094-2
Galicia-Herbada D. 2006. Origin and diversification of Thymelaea (Thymelaeaceae): inferences from a phylogenetic study based on ITS (rDNA) sequences. Plant Systematics and Evolution; 257: 159-187.
Ghedira K. 2005. Les flavonoïdes: structure, propriétés biologiques, rôle prophylactique et emplois en thérapeutique. Phytothérapie; 3 (4): 162-169.
Greenspan P, Bauer JD, Pollock SH, David Gangemi J, Mayer EP, Ghaffar A, Hargrove JL, Hartle DK. 2005. Anti-inflammatory properties of the muscadine grape (Vitis rotundifolia), Journal of Agricultural and Food Chemistry; 53: 8481. https://doi.org/10.1021/jf058015+
The Angiosperm Phylogeny Group (TAP). 1998. An ordinal classification for the families of flowering plants. Annals of the Missouri Botanical Garden; PP: 531-553. https://doi.org/10.2307/2992015
Halliwell B. 2007. Oxidative stress and cancer: have we moved forward. Biochemical Journal; 401 (1): 1-11, https://doi.org/10.1042/BJ20061131
Hamrouni-Sellami I, Rahali FZ, Rebey IB, Bourgou S, Limam F, Marzouk B. 2012. Total phenolics, flavonoids, and antioxidant activity of sage (Salvia officinalis L.) plants as affected by different drying methods. Food and Bioprocess Technology; 6(3): 806-817. https:// doi.org/10.1007/s1194 7-012-0877-7.
Iloki-Assanga SB, Lewis-Luján LM, Lara-Espinoza CL, Gil- Salido AA, Fernandez-Angulo D, Rubio-Pino JL et al. 2015. Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Research Notes; 8(1): 396.
Ioannou I, Chaaban H, Slimane M, Ghoul M. 2015. Origin of the variability of the antioxidant activity determination of food material. Biotechnology; 4: 77-92.
Ishida Y, Kitagawa K, Goto K, Ohtani H. 2005. Solid sampling technique for direct detection of condensed tannins in bark by matrix-assisted laser desorption/ ionization mass spectrometry. Rapid Commun Mass Spectrom; 19: 706-710, https://doi.org/10.1002/rcm.1845
Jamila F, Mostafa E. 2014. Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. Journal of ethnopharmacology; 154: 76-87, https://doi.org/10.1016/j.jep.2014.03.016
Javanmardi J, Stushnoff C, Locke E, Vivanco JM. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry; 83: 547-550, https://doi.org/10.1016/S0308-8146(03)00151-1
Jayaprakasha GK, Singh RP, Sakariah KK. 2001. Antioxidant activity of grape seed (Vitis vinifera) exracts on peroxidation models in vitro. Food Chemistry; 73: 285-290, https://doi.org/10.1016/S0308-8146(00)00298-3
Kanatt SR, Chander R, Sharma A. 2007. Antioxi-dant potential of mint (Mentha spicata L.) in radiationprocessed lamb meat. Food Chemistry; 100: 451-458. https://doi.org/ 10.1016/j.foodchem.2005.09.066
Khanavi M, Hajimahmoodi M, Cheraghi- Niroomand M, Kargar Z, Ajani Y, Hadjiakhoondi A et al. 2009. Comparison of the antioxidant activity and total phenolic contents in some Stachys species. African Journal of Biotechnology; 8: 1143-1147.
Kristanti AN, Tanjung M, Aminah NS. 2018. Secondary Metabolites of Aquilaria, a Thymelaeaceae Genus. Mini-reviews in organic chemistry; 15: 36-55. https://doi.org/10.2174/1570193X14666170721143041
Kumoro AC, Hasan M, Singh H. 2009. Effects of solvent properties on the Soxhlet extraction of diterpenoid lactones from Andrographis paniculata leaves. Science Asia; 35: 306-309. doi: 10.2306/scienceasia1513- 1874 .35.306, doi: 10.2306/scienceasia1513-1874.2009.35.306
Lapornik B, Prošek M, Wondra AG. 2005. Comparison of extracts prepared from plant by-products using different solvents and extraction time. Journal of Food Engineering; 71(2): 214-222, https://doi.org/10.1016/j.jfoodeng.2004.10.036
Le Floc’h E. 1983. Contribution to an ethnobotanical study of the Tunisian Flora. Tunisian Scientific Publications.
Lesjak MM, Beara IN, Or£i¢ DZ, Ana£kov GT, Balog KJ, Franci²kovi¢ MM, Mimica-Duki¢ NM. 2011. Juniperus sibirica Burgsdorf. as a novel source of antioxidant and anti-inflammatory agents. Food Chemistry; 124: 850-856, https://doi.org/10.1016/j.foodchem.2010.07.006
Lev E. 2002. Reconstructed materia medica of the Medieval and Ottoman al-Sham. Journal of Ethnopharmacology; 80(2-3): 167-179, https://doi.org/10.1016/S0378-8741(02)00029-6
Li HB, Cheng KW, Wong CC, Fan KW, Chen F & Jiang Y. 2007. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry; 102(3): 771-776, https://doi.org/10.1016/j.foodchem.2006.06.022
Lushchak VI. 2014. Free radicals, reactive oxygen species, oxidative stress and itsclassification. Chemico-Biological Interactions; 224: 164-175, https://doi.org/10.1016/ j.cbi.2014.10.016
Makitra, R. G. 2005. “Reichardt C. Solvents and Solvent Effects in Organic Chemistry, Weinheim: Wiley-VCH, 2003, 630 p.” Russian Journal of General Chemistry 75(4): 664-664.
Martins N, Barros L, Santos-Buelga C, Henriques M, Silva S, Ferreira IC. 2014. Decoction, infusion and hydroalcoholic extract of Origanum vulgare L.: Different performances regarding bioactivity and phenolic compounds. Food chemistry; 158: 73-80, https://doi.org/10.1016/ j.foodchem.2014.02.099
Mohammed FS, Kına E, Sevindik M, Doğan M, Pehlivan M. 2021. Antioxidant and antimicrobial activities of ethanol extract of Helianthemum salicifolium (Cistaceae). Indian Journal of Natural Products and Resources, 12(3): 459-462.
Mohammed FS, Sevindik M, Uysal I, Sevindik E, Akgül H. 2022. A Natural Material for Suppressing the Effects of Oxidative Stress: Biological Activities of Alcea kurdica. Biology Bulletin, 49(Suppl 2): S59-S66.
Morré DM, James MD. 2006. Anticancer activity of grape and grape skin extracts alone and combined with green tea infusions. Cancer Letters; 238-202, https://doi.org/10.1016/j.canlet.2005.07.011
Oyaizu M. 1986. Studies on products of browning reactions: antioxidative activities of browning reaction prepared from glucosamine. Japanese Journal of Nutrition; 44: 307-315, https://doi.org/10.5264/eiyogakuzashi.44.307
Özyürek M, Güçlü K., Tütem E, Başkan KS, Erçağ E, Çelik SE, Apak R. 2011. A comprehensive review of CUPRAC methodology. Analytical methods; 3(11): 2439-2453, DOI https://doi.org/10.1039/C1AY05320E
Dyer PJ, Cummings PT. 2006. Hydrogen bonding and induced dipole moments in water: Predictions from the Gaussian charge polarizable model and Car-Parrinello molecular dynamics. The Journal of chemical physics; 125(14): 144-519, https://doi.org/10.1063/1.2355485
Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry; 53: 4290-4302, https://doi.org/10.1021/ jf0502698
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine; 26(9-10): 1231-1237, https://doi.org/10.1016/S0891-5849(98)00315-3
Welton T and Reichardt, C. 2011. Solvents and solvent effects in organic chemistry. John Wiley & Sons.
Romero-Jiménez M, Campos-Sánchez J, Analla M, Muñoz-Serrano A, Alonso-Moraga Á. 2005. Genotoxicity and antigenotoxicity of some traditional medicinal herbs. Mutation Research; 585: 147-155. https://doi.org/10.1016/ j.mrgentox.2005.05.004
Sarikurkcu C, Tepe B, Daferera D, Polissiou M, Harmandar M. 2008. Studies on the antioxidant activity of the essential oil and methanol extract of Marrubium globosum subsp. globosum (lamiaceae) by three different chemical assays. Bioresource Technology; 99: 4239 4246, https://doi.org/10.1016/j.biortech.2007.08.058
Sevindik M, Onat C, Mohammed FS, Uysal İ, Koçer O. 2023. Antioxidant and antimicrobial activities of White Radish. Turkish Journal of Agriculture-Food Science and Technology, 11(2): 372-375.
Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.
Schmitt-Schillig S, Schaffer S, Weber CC, Eckert GP, Muller WE. 2005. Flavonoid and the Aging Brain. Journal of physiology and Pharmacology; 56(1): 23-36.
Shahidi F, Ambigaipalan P. 2015. Phenolics and polyphenolics in foods, beverages and spices: antioxidant activity and health effects – a review. Journal of Functional Foods; 18, 820–897. https://doi.org/10.1016/j.jff.2015.06.018.
Spigno G, Tramelli L, De Faveri DM. 2007. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering; 81: 200-208, https://doi.org/10.1016/ j.jfoodeng.2006.10.021
Szydłowska-Czerniak A, Dianoczki C, Recseg K, Karlovits G, Szłyk E. 2008. Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta; 76(4): 899-905, https://doi.org/10.1016/ j.talanta.2008.04.055
Trigui M, Hsouna AB, Tounsi S, Jaoua S. 2013. Chemical composition and evaluation of antioxidant and antimicrobial activities of Tunisian Thymelaea hirsuta with special reference to its mode of action. Industrial Crops and Products; 41: 150-157, https://doi.org/10.1016/j.indcrop.2012.04.011
Unal O, Eraslan EC, Uysal I, Mohammed FS, Sevindik M, Akgul H. 2022. Biological activities and phenolic contents of Rumex scutatus collected from Turkey. Fresenius Environmental Bulletin, 31(7): 7341-7346.
Uysal I, Koçer O, Mohammed FS, Lekesiz Ö, Doğan M, Şabik AE, Sevindik E, Gerçeker FÖ, Sevindik M. 2023. Pharmacological and Nutritional Properties: Genus Salvia. Advances in Pharmacology and Pharmacy, 11(2): 140-155
Wangensteen H, Samuelsen AB, Malterud KE. 2004. Antioxidant activity in extracts from coriander. Food Chemistry; 88: 293-297, https://doi.org/10.1016/j.foodchem.2004.01.047
Zahra MA, Phillip BO, John GD. 2016. Characterization of the polarity of subcritical water. Journal of Chromatography A. http://dx.doi.org/10.1016/j.chroma.2016.12.072
Zheng W, Wang SY. 2001. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry; 49(11): 5165-5170. https://doi.org/ 10.1021/jf010697n
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.