From Grain to Genome: Investigating Arsenic Levels in Triticum turgidum ssp durum Desf. Using GWAS
Arsenic Levels in Durum Wheat from Türkiye
DOI:
https://doi.org/10.24925/turjaf.v11i6.1148-1160.6088Keywords:
Durum wheat, Triticum Turgidum, Arsenic, Toxic, GWAS, GenomeAbstract
Producing safe and high-quality food is increasingly important, and developing durum wheat varieties with low toxicity is crucial to meeting this demand. Durum wheat breeders can achieve this goal by developing new varieties that are either more resistant to arsenic uptake or better adapted to grow in areas with high arsenic levels. High levels of arsenic can pose serious health hazards, which makes it critical to evaluate the arsenic levels. Therefore, this study evaluated the arsenic levels in diverse durum wheat genotypes, including Turkish-released cultivars and local landraces. The results showed that all genotypes had significantly low and non-toxic levels of arsenic, with an average concentration of 5.24 μg/kg. These concentrations were much lower than the minimum reported in numerous published research studies and well below the risky international standard limits for durum wheat grain (0.1 mg/kg). The study also identified two significant marker-trait associations linked to arsenic contents located on chromosomes 4A and 7B, which explained 11-17% of the phenotypic variation. These findings provide valuable insights into the arsenic levels in durum wheat genotypes and highlight the need for ongoing monitoring to ensure safe and healthy food for consumers. By conducting collaborative genome-wide association studies and employing marker-assisted selection, durum wheat breeders can accelerate the creation of new varieties that have reduced arsenic levels by identifying alleles linked to arsenic content. This study emphasizes the importance of developing low-toxicity durum wheat varieties to ensure the safety and quality of our food supply. The findings can inform breeding programs to develop such varieties and contribute to sustainable agriculture. While the study’s methodology was robust, further research is necessary to confirm and validate the genetic factors contributing to variation in arsenic content among different durum wheat genotypes.
References
Abedin MJ, Feldmann J, Meharg AA. 2002. Uptake Kinetics of Arsenic Species in Rice Plants. Plant Physiology, 128(3), 1120-1128. doi: 10.1104/pp.010733
Agency for Toxic Substances and Disease Registry (ATSDR). 2007. Toxicological Profile for Arsenic. Page: 315. Retrieved from https://www.atsdr.cdc.gov/toxprofiles/tp2-c6.pdf
Alsaleh A, Baloch FS, Derya M, Azrak M, Kilian B, Ozkan H, Nachit M. 2015. Genetic linkage map of Anatolian durum wheat derived from a cross of Kunduru-1149 x Cham1. Plant Mol. Biol. Rep. 33 (2), 209e220. DOI 10.1007/s11105-014-0749-6
Alsaleh A, Baloch FB, Nachit M, Özkan H. 2016. Phenotypic and genotypic intra-diversity among Anatolian durum wheat “Kunduru” landraces, Biochemical Systematics and Ecology, Volume 65, 2016, Pages 9-16, ISSN 0305-1978, https://doi.org/10.1016/j.bse.2016.01.008
Alsaleh A, Bektas H, Baloch FS, Nadeem MA, Özkan H. 2022a. Turkish durum wheat conserved ex-situ and in situ unveils a new hotspot of unexplored genetic diversity. Plant Genetic Resources, Crop Science. 2022; 62:1200–1212. DOI: 10.1002/csc2.20723
Alsaleh A, Baloch FA, Sesiz U, Nadeem MA, Hatipoglu R, Erbakan M, Özkan H. 2022b. Marker-assisted selection and validation of DNA markers associated with cadmium content in durum wheat germplasm, crop and pasture science, 73(7–8), 943–956. doi:10.1071/CP21484
Alsaleh A. 2022c. SSR-based genome-wide association study in Turkish durum wheat germplasms revealed novel QTL of accumulated platinum, Molecular Biology Reports. https://doi.org/10.1007/s11033-022-07720-7
Baloch FS, Alsaleh A, Shahid MQ, Çiftçi V, Sáenz de Miera LE, Aasim M, Nadeem MA, Aktaş H, Özkan H, Hatipoğlu R. 2017. A whole genome DArTseq and SNP analysis for genetic diversity assessment in durum wheat from Central Fertile Crescent. PLoS ONE 12(1):e0167821. https://doi.org/10.1371/journal. pone.0167821
Bradbury P, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y, Buckler ES. 2007. TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635. https://doi.org/10.1093/bioinformatics/btm308
Canadian Food Inspection Agency. 2020. Levels of Arsenic in Foods. Retrieved from https://inspection.canada.ca/food-safety-for-industry/food-chemistry-and-microbiology/food-safety-testing-bulletin-and-reports/total-arsenic-and-arsenic-speciation-in-infant-foo/eng/1665086574334/1665086574693
Corguinha APB, de Souza GA, Gonçalves VC, Carvalho CA, de Lima WEA, Martins FAD, Yamanaka CH, Francisco EAB, Guilherme LRG. 2015. Assessing arsenic, cadmium, and lead contents in major crops in Brazil for food safety purposes, Journal of Food Composition and Analysis, Volume 37, Pages 143-150, ISSN 0889-1575, https://doi.org/10.1016/j.jfca.2014.08.004
Cubadda F, Ciardullo S, D’Amanto M, Ragi A, Aureli F, Carcea M. 2010. Arsenic contamination of the environment–food chain: phytoavailable arse- nic in Italian agricultural soils and as a source of inorganic arsenic in the diet. Journal of Agricultural and Food Chemistry 58 (18), 10176–10183. https://doi.org/10.1021/jf102084p
Doyle J J, Doyle J L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11–15. https://webpages.charlotte.edu/~jweller2/pages/ BINF8350f2011/BINF8350_Readings/Doyle_plantDNAextractCTAB_1987.pdf
Ellegren H. 2004. Microsatellites: simple sequences with complex evolution. Nat Rev Genet 5, 435–445. https://doi.org/10.1038/nrg1348
European Union, 2015. Commission Regulation (EU) No 2015/1006 of 25 June 2015 amending Regulation (EC) No 1881/2006 as regards maximum levels of inorganic arsenic in foodstuffs. Annex II of the regulation specifies the maximum levels of inorganic arsenic in various foodstuffs, including durum wheat. Available from https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A32015R1006
FAO and WHO, 2018. CODEX Alimentarius Commission, Joint FAO/WHO Food Standards Programme. Codex Committee on Contaminants in Foods. Working document for information and use in discussions related to contaminants and toxins in the GSCTFF. Twelfth session, Utrecht, The Netherlands, 12-16 March 2018
FAO, 2020. Food and Agriculture Organization of the United Nations. FAOSTAT database, Production Quantity of Wheat in 2020
Frouin J, Labeyrie A, Boisnard A, Sacchi GA, Ahmadi N. 2019. Genomic prediction offers the most effective marker assisted breeding approach for ability to prevent arsenic accumulation in rice grains. PLoS ONE 14(6): e0217516. https://doi.org/10.1371/journal.pone.0217516
Giraldo P, Royo C, González M, Carrillo JM, Ruiz M. 2016. Genetic Diversity and Association Mapping for Agromorphological and Grain Quality Traits of a Structured Collection of Durum Wheat Landraces Including subsp. durum, turgidum and diccocon. PLoS ONE 11(11): e0166577. https://doi.org/10.1371/journal.pone.0166577
Hirzel J, Retamal-Salgado J, Walter I, Matus I. 2019. Residual effect of cadmium applications in different crop rotations and environments on durum wheat cadmium accumulation. Journal of Soil and Water Conservation January 2019, 74 (1) 41-50; DOI: https://doi.org/10.2489/jswc.74.1.41
Hossain M, Piyatida P, da Silva JAT, Fujita M. 2012. Molecular Mechanisms of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation. Journal of Botany, 2012, 1-37. https://doi.org/10.1155/2012/872875
Huang M, Zhou S, Sun B, Zhao Q. 2008. Heavy metals in wheat grain: Assessment of potential health risk for inhabitants in Kunshan, China. Science of the Total Environment 405, 54–61. DOI: 10.1016/j.scitotenv.2008.07.004
IARC (International Agency for Research on Cancer). 2004. Some drinking-water disinfectants and contaminants, including arsenic. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 84, 269-477. https://pubmed.ncbi.nlm.nih.gov/15645577/
Iqbal M J, Shams N, Fatima K. 2022. Nutritional Quality of Wheat. Wheat. Intech Open. DOI: 10.5772/intechopen.104659.
Kaler AS, Purcell LC. 2019. Estimation of a significance threshold for genome-wide association studies. BMC Genomics 20, 618 (2019). https://doi.org/10.1186/s12864-019-5992-7
Kaya H B, Cetin O, Kaya H S, Sahin M, Sefer F, Tanyolac B. 2016. Association Mapping in Turkish Olive Cultivars Revealed Significant Markers Related to Some Important Agronomic Traits Biochem Genet, DOI 10.1007/s10528-016-9738-9.
Liu WX, Liu JW, Wu MZ, Li Y, Zhao Y, Li SR. 2009. Accumulation and Translocation of Toxic Heavy Metals in Winter Wheat (Triticum aestivum L.) Growing in Agricultural Soil of Zhengzhou, China. Bull Environ Contam Toxicol 82, 343–347 (2009). https://doi.org/10.1007/s00128-008-9575-6
Moon K, Guallar E, Navas-Acien A. 2012. Arsenic exposure and cardiovascular disease: an updated systematic review. Curr Atheroscler Rep. 2012 Dec; 14 (6):542-55. doi: 10.1007/s11883-012-0280-x.
Nachit MM, Elouafi I, Pagnotta A, El Saleh A, Iacono E, Labhilili M, Asbati A, Azrak M, Hazzam H, Benscher D, Khairallah M, Ribaut JM, Tanzarella OA, Porceddu E, Sorrells ME. 2001. Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum). Theoretical and Applied Genetics 102(2–3), 177–186. doi:10.1007/s001220051633
Nadeem MA, Nawaz MA, Shahid MQ, Do˘gan Y, Comertpay G, YıldızM, Hatipoglu R, Ahmad F, Alsaleh A, Labhane N, Özkan H, Chung G, Baloch FS. 2018. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology & Biotechnological Equipment 32(2), 261–285. doi:10.1080/13102818.2017.1400401
Pompa C, D’Amore T, Miedico O, Preite C, Chiaravalle AE. 2021. Evaluation and Dietary Exposure Assessment of Selected Toxic Trace Elements in Durum Wheat (Triticum durum) Imported into the Italian Market: Six Years of Official Controls. Foods. 2021 Apr 4;10(4):775. doi: 10.3390/foods10040775
Schuelke M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18(2), 233–234. https://doi.org/10.1038/72708.
Shahid M, Dumat C, Khalid S, Schreck E, Xiong T, Niazi NK. 2017. Foliar heavy metal uptake, toxicity and detoxification in plants: A comparison of foliar and root metal uptake. J Hazard Mater. 2017 Mar 5;325:36-58. Epub 2016 Nov 24. PMID: 27915099. doi: 10.1016/j.jhazmat.2016.11.063
Shewry PR, Sandra J H. 2015. The contribution of wheat to human diet and health, Food Energy Secur. 2015 Oct; 4(3): 178–202. doi: 10.1002/fes3.64
Shi GL, Li DJ, Wang YF, Liu CH, Hu ZB, Lou LQ, Rengel Z, Cai QS. 2019. Accumulation and distribution of arsenic and cadmium in winter wheat (Triticum aestivum L.) at different developmental stages, Science of The Total Environment, Volume 667, 2019, Pages 532-539, ISSN 0048-9697, https://doi.org/10.1016/j.scitotenv.2019.02.394
Skrbic B, Onjia A. 2007. Multivariate analyses of microelement contents in wheat cultivated in Serbia. Food Control 18, 338–345. https://doi.org/10.1016/j.foodcont.2005.10.017
Tam V, Patel N, Turcotte M, Bossé Y, Paré G, Meyre D. 2019. Benefits and limitations of genome-wide association studies. Nat Rev Genet 20, 467–484 (2019). https://doi.org/10.1038/s41576-019-0127-1
U.S. Food and Drug Administration. 2016. Code of Federal Regulations Title 21, Part 109.30: Arsenic in Food. Federal Register, 81(63), 15523-15528
Vergine M, Aprile A, Sabella E, Genga A, Siciliano M, Rampino P, Lenucci MS, Luvisi A, Bellis LD. 2017. Cadmium concentration in grains of durum wheat (Triticum turgidum L. subsp. durum). Journal of Agricultural and Food Chemistry 65(30), 6240–6246. doi:10.1021/ acs.jafc.7b01946
Vieira ML, Santini L, Diniz AL, Munhoz Cde F. 2016. Microsatellite markers: what they mean and why they are so useful. Genet Mol Biol. 2016 Jul-Sep;39(3):312-28. doi: 10.1590/1678-4685-GMB-2016-0027. Epub 2016 Aug 4. PMID: 27561112; PMCID: PMC5004837.
WHO, 2022. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/arsenic
Zhang WD, Liu DS, Tian JC, He FL. 2009. Toxicity and accumulation of arsenic in wheat (Triticum aestivum L.) varieties of China, FYTON ISSN 0031 9457, (2009) 78: 147-154. https://doi.org/10.32604/phyton.2009.78.147
Zhao FJ, Stroud JL, Eagling T, Dunham SJ, McGrath SP, Shewry PR. 2010. Accumulation, Distribution, and Speciation of Arsenic in Wheat Grain, Environmental Science & Technology 2010 44 (14), 5464-5468, DOI: 10.1021/es100765g
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