Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line
DOI:
https://doi.org/10.24925/turjaf.v11is1.2499-2504.6410Keywords:
Pumpkin seed oil, Pentylenetetrazole, Oxidative stress, Nitrosative stress, HT-22 cellsAbstract
Recent studies have shown the positive effects of Cucurbita pepo L. (pumpkin) seed oil (PSO) in different disease models. However, the effect of PSO on neurological diseases has not been clarified yet. Therefore, this study aims to elucidate the effects of BBS on pentylenetetrazole (PTZ)-induced neuronal damage and the possible roles of oxidative and nitrosative stress in this effect in vitro. The HT-22 hippocampal neuronal cell line was used in the study. Cell survival after PTZ-induced neuronal damage was evaluated with the XTT test in the groups. While the effects of BBS on total antioxidant status (TAS) and total oxidant status (TOS) after PTZ were measured with colorimetric commercial kits, its effects on neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) levels were also determined by ELISA kits. In light of the data obtained, it was found that pre-treatment with PSO prevented the decrease in cell survival after exposure to PTZ. In addition, it has been found that PSO normalizes the increase in TOS, nNOS, and NO in neuronal cells after PTZ. As a result, it was determined that the treatment of neuronal cells with PSO prevented neuronal damage caused by PTZ and showed neuroprotective properties. It is thought that PSO may achieve these effects through oxidative and nitrosative systems. Enrichment of a daily diet with PSO might be beneficial in reducing the risks of neurological diseases.
References
Ahlatcı A, Yıldızhan K, Tülüce Y, Bektaş M. 2022. Valproic Acid Attenuated PTZ-induced Oxidative Stress, Inflammation, and Apoptosis in the SH-SY5Y Cells via Modulating the TRPM2 Channel. Neurotox Res, 40(6):1979-1988. doi: 10.1007/s12640-022-00622-3
El-Mosallamy AE, Sleem AA, Abdel-Salam OM, Shaffie N, Kenawy SA. 2012. Antihypertensive and Cardioprotective Effects of Pumpkin Seed Oil. J Med Food, 15:180–189. doi: 10.1089/JMF.2010.0299
Eraslan G, Kanbur M, Aslan Ö, Karabacak M. 2013. The antioxidant effects of pumpkin seed oil on subacute aflatoxin poisoning in mice. Environ Toxicol, 28:681–688. doi: 10.1002/TOX.20763
Fruhwirth GO, Hermetter A. 2007. Seeds and oil of the Styrian oil pumpkin: Components and biological activities. Eur J Lipid Sci Technol, 109:1128–1140. doi: 10.1002/EJLT.200700105
Gossell-Williams M, Davis A, O’Connor N. 2006. Inhibition of Testosterone-Induced Hyperplasia of the Prostate of Sprague-Dawley Rats by Pumpkin Seed Oil. J Med Food, 9:284–286. doi: 10.1089/JMF.2006.9.284
Irnawati I, Riyanto S, Martono S, Windarsihe A, Rohman A. 2022. Physicochemical properties and antioxidant activities of pumpkin seed oil as affected by different origins and extraction methods. J Appl Pharm Sci, 12:115–122. doi: 10.7324/JAPS.2022.120312
Makni M, Fetoui H, Gargouri NK, Garoui el M, Jaber H, Makni J, Boudawara T, Zeghal N. 2008. Hypolipidemic and hepatoprotective effects of flax and pumpkin seed mixture rich in omega-3 and omega-6 fatty acids in hypercholesterolemic rats. Food Chem Toxicol, 46:3714–3720. doi: 10.1016/J.FCT.2008.09.057
Morrison MC, Mulder P, Stavro PM, Suárez M, Arola-Arnal A, van Duyvenvoorde W, Kooistra T, Wielinga PY, Kleemann R. 2015. Replacement of Dietary Saturated Fat by PUFA-Rich Pumpkin Seed Oil Attenuates Non-Alcoholic Fatty Liver Disease and Atherosclerosis Development, with Additional Health Effects of Virgin over Refined Oil. PLoS One, 10:e0139196. doi: 10.1371/JOURNAL.PONE.0139196
Nicoletti M. 2012. Nutraceuticals and botanicals: overview and perspectives. Int J Food Sci Nutr, 63:2–6. doi: 10.3109/09637486.2011.628012
Patel S. 2013. Pumpkin (Cucurbita sp.) seeds as nutraceutic: A review on status quo and scopes. Med J Nutrition Metab, 6:183–189. doi: 10.1007/S12349-013-0131-5/METRICS
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. 2017. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev, 2017: 8416763. doi: 10.1155/2017/8416763
Rouag M, Berrouague S, Djaber N, Khaldi T, Boumendjel M, Taibi F, Abdennour C, Boumendjel A, Messarah M. 2020. Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach. Afr Health Sci, 20:413–425. doi: 10.4314/AHS.V20I1.48
Salim S. 2017. Oxidative Stress and the Central Nervous System. J Pharmacol Exp Ther, 360:201. doi: 10.1124/JPET.116.237503
Šamec D, Loizzo MR, Gortzi O, Çankaya İT, Tundis R, Suntar İ, Shirooie S, Zengin G, Devkota HP, Reboredo-Rodríguez P, Hassan STS, Manayi A, Kashani HRK, Nabavi SM. 2022. The potential of pumpkin seed oil as a functional food—A comprehensive review of chemical composition, health benefits, and safety. Compr Rev Food Sci Food Saf, 21:4422–4446. doi: 10.1111/1541-4337.13013
Shaban A, Sahu RP. 2017. Pumpkin Seed Oil: An Alternative Medicine. Int J Pharmacogn Phytochem Res, 9:11. doi: 10.25258/PHYTO.V9I2.8066
Siró I, Kápolna E, Kápolna B, Lugasi A. 2008. Functional food. Product development, marketing and consumer acceptance A review. Appetite, 51:456–467. doi: 10.1016/ J.APPET.2008.05.060
Stevenson DG, Eller FJ, Wang L, Jane JL, Wang T, Inglett GE. 2007. Oil and Tocopherol Content and Composition of Pumpkin Seed Oil in 12 Cultivars. J Agric Food Chem, 55:4005–4013. doi: 10.1021/JF0706979
Taskiran AS, Ergul M, Gunes H, Ozturk A, Sahin B, Ozdemir E. 2021. The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line. Cell Mol Neurobiol, 41:173–183. doi: 10.1007/S10571-020-00956-6/FIGURES/9
Tastemur Y, Gumus E, Ergul M, Ulu M, Akkaya R, Ozturk A, Taskiran AS. 2020. Positive effects of angiotensin-converting enzyme (ACE) inhibitor, captopril, on pentylenetetrazole-induced epileptic seizures in mice. Trop J Pharm Res, 19:637–643. doi: 10.4314/TJPR.V19I3.26
Yildizhan K. 2020. Protective role of selenium against bisphenol-A induced oxidative stress, cytokine generation and apoptosis in SH-SY5Y neuronal cell line. J Cell Neurosci Oxid Stress, 12(3): 955-962. doi: 10.37212/jcnos.1005692
Yildizhan K, Gunes H, Taskiran AS. 2023. Effect of Anakinra and Infliximab on Oxidative Stress and Caspase Activation in PTZ-Induced Acute Seizure in Rats. Eastern Journal of Medicine, 28(1): 75-81. doi: 10.5505/ejm.2023.84669
Yildizhan K, Naziroğlu M. 2019. Microglia and its role in neurodegenerative diseases. Journal of Cellular Neuroscience and Oxidative Stress, 11(2): 861-873. doi: 10.37212/jcnos.683407
Yildizhan K, Ozturk A. 2022. Quipazine treatment exacerbates oxidative stress in glutamate-induced HT-22 neuronal cells. The European Research Journal, 8(4): 521-528. doi: 10.18621/eurj.1027423
Yüncü M, Eralp A, Celik A. 2006. Effect of aged garlic extract against methotrexate-induced damage to the small intestine in rats. Phyther Res, 20:504–510. doi: 10.1002/ptr.1896
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
