Effectiveness of Phosphorous acid, Bacillus subtilis and Copper Compounds on Apple cv. Gala with M9 Rootstock in the Control of Fire Blight
DOI:
https://doi.org/10.24925/turjaf.v11is1.2595-2600.6533Keywords:
fire blight, control, copper, plant defenceAbstract
Necrogenic Gram-negative bacterium Erwinia amylovora, which causes economic losses especially in pome fruits such as apple, pear, quince and loquat, some berries and many ornamental plants, causes fire blight disease. Copper compounds are used extensively in disease control programs and they can cause toxicproblems in terms of plant and environmental health. In addition, the formation of resistance to copper in the pathogen is frequently observed. In this study, plant activator phosphorous acid and biological control agent Bacillus subtilis, and 3 different copper compounds, Copper sulfate, Copper oxychloride and Copper hydroxide which are commonly used against E. amylovora, on apple cv. Gala with M9 rootstock were evaluated comparatively. When the new season shoot lengths of 3-year-old plants with homogeneous growth reached 20-25 cm, chemicals and B. subtilis were applied first time before one week ago from the pathogen inoculation, and after 2 times with 1 week intervals. The youngest two leaves at tips of actively growing terminal plant shoots were inoculated by cutting off using scissors dipped in suspension of E. amylovora str. EaARADY5 containing 108 CFU ml-1. Disease assessments were made after the disease severity (%) was determined on the basis of shoot blight after symptom development of the disease stopped, and the results were found to be statistically significant (p<0.05). According to the findings, while the highest effect was obtained by copper oxychloride with 69% effectiveness among all applications, the lowest effectiveness was obtained with 43.5% copper sulfate. It has been revealed that the use of the most effective of the copper compounds in field applications will result in less exposure to chemicals in terms of human and environmental health, and that B. subtilis and phosphorous acid can be used significantly in the integrated control of fire blight.
References
Abbasi PA, Weselowski B. 2014. Influence of foliar sprays of Bacillus subtilis QST 713 on development of early blight disease and yield of field tomatoes in Ontario. Can J Plant Pathol 36(2):170–178.
Abbott WS. 1925. A method of computing the effectiveness of an insecticide. J. econ. Entomol, 18(2), 265-267.
Aldwinckle H, Bhaskara RMV, Norelli J. 2002. Evaluation Of Control Of Fire Blight Infection of Apple Blossoms and Shoots with SAR Inducers, Biological Agents, A Growth Regulator, Copper Compounds And Other Materials. Acta Hort. 590: 325-331.
Anonymous, 2020. Tarım Ürünleri Piyasaları, https://arastirma.tarimorman.gov.tr. Erişimtarihi:10.11.2020.
Bereswill S, Pahl A, Bellemann P, Zeller W, Geider K. 1992. Sensitive and species- specific detection of Erwinia amylovora by polymerase chain reaction analysis. App. Environ. Microbial, 58:3522-3526.
Bonasera JM, Kim JF, Beer SV. 2006. PR genes of apple: identification and expression in response elicitors and inoculation with Erwinia amylovora, BMC Plant Biol. doi: 10, 1186/1471-2229-6-23.
Borriss R. 2011. Use of plant-associated Bacillus strains as biofertilizers and biocontrol agents in agriculture. In: Maheshwari D (ed) Bacteria in agrobiology: plant growth responses. Springer, Berlin, pp 41–76
Butt H, Bastas KK. 2021. Antagonistic Activity of Bacillus spp. Against Fire Blight Disease In vitro and In planta. Turkish Journal of Agriculture-Food Science and Technology, 9, 2486-2492.
Deckers T, Schoofs H. 2007. Status of the pear production in Europe. In X International Pear Symposium 800 (pp. 95-106).
Düzgüneş O, Kesici T, Gürbüz F. 1983. İstatistik Metodları. AÜZF Yayınları No:861. Ankara.
FAO 2018. Birleşmiş Milletler Gıda ve Tarım Örgütü. FAO STAT. http://www.fao.org/faostat, Erişim tarihi. 11.01.2018.
Fernando WGD, Jones AL. 1999. Prohexadione-Ca: A tool for reducing secondary fire blight infections. Acta Hort. 489: 597-600. DOI: 1017660/ActaHortic.1999.489.103.
Fousia S, Paplomatas EJ, Tjamos SE. 2016. Bacillus subtilis QST 713 confers protection to tomato plants against Pseudomonas syringae pv. tomato and induces plant defence-related genes. J Phytopathol 164:264–270.
Ibrahim YE, Saleh AA, El-Komy MH, Al-Saleh MA. 2016. Bacillus subtilis QST 713, copper hydroxide, and their tank mixes for control of bacterial citrus canker in Saudi Arabia. J Cit Pathol 3(1):1–6
Johnson KB, Temple T. 2013. Evaluation of strategies for fire blight control in organic pome fruit without antibiotics. Plant Dis 97:402–409.
Koch R, 1884. Die Aetiologie der Tuberkulose. Mittheilungen aus dem Kaiserlichen Gesundheitsamte. 2:1–88.
Klement Z, Rudolph K, Sands D. 1990. Methods in Phytobacteriology, Akademiai Kiado, p.112.
Lamichhane JR, Osdaghi E, Behlau F, Köhl J, Jones JB, Aubertot JN. 2018. Thirteen decades of antimicrobial copper compounds applied in agriculture. A review. Agron. Sustain. Dev. 38:28
ldwinckle H, Bhaskara RMV, Norelli J. 2002. Evaluation ofcontrol of fire blight infection of apple blossoms and shoots with SAR inducers, biological agents,a growth regulator, copper compounds and other materials. Acta Hort. 590: 325-331.
McGhee GC, Sundin GW. 2011. Evaluation of kasugamycin for fire blight management, effect on nontarget bacteria, and assessment of kasugamycin resistance potential in Erwinia amylovora. Phytopathology 101:192-204.
McManus PD. 2014. Does a drop in the bucket make a splash? Assessing the impact of antibiotic use on plants. Curr. Opin. Microbiol. 19:76-82.
Momol MT, Yegen O, Basım H, Zachowski MA, Rudolph K, Purdy LH. 1991. Development of fire blight epidemics and control measures in pear orchards in Turkey. Phytopathology. 81: 1137–1138.
Myung IS, Lee JY, Yun MJ, Lee YH, Park DH, Oh CS. 2016. Fire blight of apple caused by Erwinia amylovora, a new disease in Korea. Plant Dis. 100:1774.
Ninot A, Aletà N, Moragrega C, Montesinos E. 2002. Evaluation of a reduced copper spraying program to control bacterial blight of walnut. Plant Dis. 86:583-587.
Norman DJ, Chen J, Yuen JMF, Mangravita-Novo A, Byrne D, Walsh L. 2006. Control of bacterial wilt of geranium with phosphorous acid. Plant disease, 90(6), 798-802.
Ordax M, Marco-Noales E, López MM, Biosca EG. 2006. Survival strategy of Erwinia amylovora against copper: induction of the viable-but-nonculturable state. Applied and environmental microbiology, 72(5), 3482-3488.
Russell DW, Sambrook J. 2001. Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Cold Spring Harbor, NY, p1890.
Ryu DK, Adhikari M, Choi DH, Jun KJ, Kim DH, Kim CR, ... & Park DH. 2023. Copper-Based Compounds against Erwinia amylovora: Response Parameter Analysis and Suppression of Fire Blight in Apple. The Plant Pathology Journal, 39(1), 52.
Saygılı H, Şahin F, Aysan Y. 2008. Bitki Bakteri Hastalıkları. Meta Basım, İzmir, 61-68:177-178.
Saygılı H, Üstün N. 1995. Studies on Effectiveness of Some Chemicals to Fire Blight Pathogen Erwinia amylovora (Burrill) Winslow et al. In VII International Workshop on Fire Blight 411 (pp. 331-336).
Schaad NW, Jones JB, Chun W. 2001. Laboratory guide for the identification of plant pathogenic bacteria. Ed. 3, American Phytopathological Society (APS Press).
Smith T. 2012. Improving the management of two critical pome fruit diseases. Tree Fruit Research Commission Final Report
Smith T. 2015. Improving the management of two critical pome fruit diseases. Tree Fruit Research Commission Final Report
Yüce HG, Tosun N, Türküsay H. 2020. Sanayi Domatesinde Bakteriyel Leke (Xanthomonas axonopodis pv. vesicatoria) ve Geç Yanıklık (Phytophthora infestans) Hastalıklarına Karşı Farklı İlaçlama Programlarının Etkinliklerinin Araştırılması. Ege Üniversitesi Ziraat Fakültesi Dergisi, 61-69.
Zhao YQ, Tian YL, Wang LM, Geng GM, Zhao WJ, Hu BS, Zhao, YF. 2019. Fire blight disease, a fastapproaching threat to apple and pear production in China. J. Integr. Agric. 18:815-820.
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