Effect of Different Media on Short Term Storage of Leaping Mullet (Liza saliens (Risso, 1810)) Sperm

Serhat Engin; Muammer Kürşat Fırat; Osman Özden; Cüneyt Suzer; Müge Hekimoğlu; Onurkan Antepli; Şahin Saka

doi:10.24925/turjaf.v8i4.996-1000.3262

Authors

Serhat Engin Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0002-1663-1769
Muammer Kürşat Fırat Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0003-1470-209X
Osman Özden Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0003-3638-6657
Cüneyt Suzer Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0003-3535-2236
Müge Hekimoğlu Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0003-4685-7475
Onurkan Antepli Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0002-7242-8925
Şahin Saka Aquaculture Department, Faculty of Fisheries, Ege University, 35100 İzmir http://orcid.org/0000-0001-7300-4860

DOI:

https://doi.org/10.24925/turjaf.v8i4.996-1000.3262

Keywords:

Short – term storage, Liza saliens, leaping mullet, sperm, viability

Abstract

The present study aimed to evaluate the activation characteristics of the leaping mullet spermatozoa and diluted with four different media (D1, D2, D3, D4) for 250 h at 4±2Â°C. The semen was collected from nine wild fish in Homa Lagoon, İzmir, Turkey in July, during reproductive season. In each trial (n = 9), semen samples were collected and mixed to reduce sperm quality variation to obtain the required gametes for the experiment. The semen was activated with natural seawater (salinity of 34, pH 7.9 and 4±2Â°C) to determine: motility time, motility rate and viability of sperm. Dilution with inactivation media 1:3 increased motility time of diluted semen than control group. At the end of the experiment, no significant difference was found for the duration of survival in the D2 and D4 groups. The highest survival rate was found in D2 group.