Metagenomic Analysis of the Fungal Microbiome in Contaminated Soil with Heavy Metal
DOI:
https://doi.org/10.24925/turjaf.v11i9.1671-1677.6261Keywords:
Metagenomic Analysis, Fungal Microbiota, Heavy metal, Microbiome, Samsun OIZAbstract
Heavy metal contamination, which has become one of the most important problems of today with the developing industrial activities, is a serious threat to all living organisms. Some fungi have resistance mechanisms against heavy metals and this is recognized as a sustainable approach for remediation processes. The aim of this study was to shed light on biological remediation approaches for heavy metals by identifying resistant fungal microbiota in areas with high levels of heavy metal contamination. In this study, Cr, Mn, Ni, Cu, Zn, Cd, Pb, As and V heavy metal ratios in soil samples taken from Samsun Organized Industrial Zone, which has high heavy metal pollution, were determined by ICP-OES. Soil fungal microbiota were determined using metagenomic next generation sequencing technology. Illumina MiSeq technology was used for metagenomic sequencing and QIIME 2 2017.4 was used for statistical microbiome analysis. In the heavy metal contaminated study area, Mortierella 53.90%, Halokirschsteiniothelia 18.01%, Rhizopogon 2.74%, Cladosporium 1.88%, Aspergillus 1.62% and Gibberella 1.12% genera were detected at the highest rates, respectively. The results show that taxa resistant to excess heavy metal concentrations are dominant in the environment.
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