Investigation of Antioxidant and Immune System Parameters in Oreochromis niloticus Fed with Organic and Inorganic Selenium Supplementation Feeds

Abstract

In this study, antioxidant enzyme activities and immun system parameters of liver tissue of Oreochromis niloticus fed with organic (Sel-Plex®-2000, Alltech, USA) and inorganic selenium (Na2SeO3.5H2O - Sodium selenite pentahydrate-FLUCA) supplemented feed were investigated. The fish with average weights of 12.62 ± 0.11 g were placed in aquariums and fed for 75 days in 5 different application groups (Control; Org1.5; Org3.0; Inorg1.5; Inorg3.0). At the end of the trial period, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), lysozyme (LYZ) and myeloperoxidase (MPO) activities and MDA level in the liver tissue and selenium accumulation in the muscle was determined. The antioxidant enzyme activities and immune system parameters in liver were increased in all Se groups compared to control. Se levels in muscle tissues significantly increased in all tested selenium groups when compared to control. In fish fed with 3mg/kg of organic selenium, muscle selenium levels were found to be higher than other groups.