Determination of Genetic Diversity Using Phenotypic and Molecular Methods among Bean Genotypes (Phaseolus vulgaris L.) in Lake Van Basin

Abstract

The genetic relationships among 95 bean genotypes collected from different districts of Lake-Van Basin were determined by both phenotypic and molecular markers. In the phenotypic method, 71 morphological traits were examined and those with high correlations were excluded from the evaluation; then 61 measurements or observations of bean genotypes were employed. In the molecular method, 219 polymorphic ISSR markers obtained from 28 primers and 76 polymorphic RAPD markers obtained from 10 primers were employed. The genetic relationships among the bean genotypes were studied by examining dendrogram resulted from Euclidean distance obtained from phenotypic data and Euclidean distance and Jaccard‘s coefficient obtained from molecular data. In the phenotypic characterization, the genotypes were originated 69.5 % to South America (Andean) and 30.5 % to Central America (Mesoamerican), and there were high genetic diversity among the genotypes. In the evaluation of combined phenotypic and molecular data, it was observed that the genotypes originated from Andean and Mesoamerican; dwarf and climbing genotypes; genotypes with white, other one-colored, and mottled seeds were clustered separately.