Determination of The Prevalence of Potato Soft Rot and Blackleg Disease in Potato Production Areas of Tokat Province and Identification of Disease Causal Agent

Abstract

This study was aimed at identification and prevalence of potato soft rot and black leg disease agent in the potato production areas of Tokat province. In March-August 2018, 67 field surveys were carried out in Central, Turhal, Zile, Pazar, Erbaa, Niksar, Artova and Basciftlik districts of Tokat. The disease incidences were 0.25%, 0.33%, 0.31%, 0.5%, 1%, and 8% in Central, Erbaa, Niksar, Pazar, Turhal, and Zile district, respectively. In Artova and Basciftlik districts, no disease was encountered. The following tests, pectolytic activity on potato, gram reaction, catalase, oxidase, growth at 37°C and 39°C, salt tolerance, hypersensitivity reaction were applied to isolates obtained from diseased plant and tuber samples. In the PCR assay, 19 isolates were produced 434 bp product with Y1/Y2 primers specific to Pectobacterium spp., and 3 isolates were produced 420 bp product with ADE1/ADE2 primers specific to Dickeya spp. The isolates resulted positive with Y1/Y2 primers weren‘t produced PCR product with ECA1/ECA2 primers specific to Pectobacterium atrosepticum. According to this, 19 isolates were identified as Pectobacterium carotovorum. With this study, the causal agent of potato blackleg and soft rot disease have been identified in the potato production areas of Tokat. Further studies will be conducted to determine the species and subspecies level of the pathogens using specific primers.