Micropropagation of Spathiphyllum with Temporary Immersion Bioreactor System

Abstract

Turkey has an advantageous position for the cultivation of ornamental plants for reasons such as favourable conditions, proximity to markets and cheap labour. In addition to classical production methods, biotechnological methods are used to meet the demand of Spathiphyllum, which is an indoor plant. In recent years, it has been started to be used in micropropagation of plants called temporary immersion system as well as classical tissue culture systems. Within the scope of the present study, micropropagation and rooting studies were carried out using classical tissue culture system and Plantform, one of the temporary immersion bioreactor systems, in the commercially important Spathiphyllum ‘Chico‘ genotype. MS medium containing 1 mg L-1 6-Benzylaminopurine (BAP) was used in micropropagation experiments established in both systems, and Murashige and Skoog (MS) medium containing 1 mg L-1 Indole-3-butyric acid (IBA) was used in rooting experiments. The results of micropropagation and rooting have been found to be successful in both systems. As a result of the screening with Sequence-Related Amplified Polymorphism (SRAP) markers, it was determined that there were no genetic differences in the plants that were reproduced and rooted.