Mitigation of Chilling Stress Effects on Eggplant Seedlings by Exogenous Application of Melatonin

Abstract

The purpose of this study was to explore the possibilities of improving chilling stress tolerance of eggplant seedlings through exogenous melatonin (MEL) application. Eggplant (Hadrian F1) seedlings were treated with various concentrations (0, 1, 5 or 25 μM) of MEL via soil drench after which they were subjected to chilling stress at 5°C/10°C (night/day) for 3 days. Following stress imposition, the efficacy of MEL applications on enhancing chilling stress tolerance was determined by several physical and physiological measurements and biochemical analyses. The results demonstrated that exogenous application of MEL alleviated the adverse effects of chilling stress in eggplant seedlings. Among the MEL concentrations tested, 5 µM was determined as the most effective concentration since antioxidant enzyme (CAT, POX and APOX) and photosynthetic activities increased while visual and membrane damage decreased in 5 µM MEL-treated seedlings. Also, these results are the first experimental evidence that exogenous application of MEL could improve chilling stress tolerance in eggplant, but further detailed studies are necessary to better understand the mechanism in acquiring chilling tolerance.