Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries

Mahdeb Nadia; Khadidja Attafi; Souha Bouhouhou; Allouni Rima; Abdelouahab Bouzidi

doi:10.24925/turjaf.v11i10.1862-1870.5768

Authors

Mahdeb Nadia Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas, Setif 1 – 19000 Setif, Algeria https://orcid.org/0000-0003-1568-8752
Khadidja Attafi Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas, Setif 1 – 19000 Setif, Algeria https://orcid.org/0009-0004-7036-4683
Souha Bouhouhou Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas, Setif 1 – 19000 Setif, Algeria https://orcid.org/0009-0003-7734-8216
Allouni Rima Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas, Setif 1 – 19000 Setif, Algeria https://orcid.org/0000-0001-6098-5399
Abdelouahab Bouzidi Department of Biochemistry, Faculty of natural sciences and life, University Ferhat Abbas, Setif 1 – 19000 Setif, Algeria https://orcid.org/0000-0002-4370-0600

DOI:

https://doi.org/10.24925/turjaf.v11i10.1862-1870.5768

Keywords:

Ruta montana L., toxicity, LD50, liver, kidneys, ovaries.

Abstract

Ruta montana L. is an annual aromatic plant of the family rutaceae. Quantitative analysis of the methanolic crude extract of Ruta montana L. yielded 8.43%, whereas the qualitative analysis revealed the presence of alkaloid or coumarin. The Litchfield and Wilcoxon method calculated the LD₅₀ of the crude methanolic extract of Ruta montana L. in Wistar albino female rats at 393.18 mg/kg. This allows the plant to be classified as moderately toxic. The subacute toxicity study of the methanolic crude extract of Ruta montana L. in female Wistar albino rats treated with 100 mg/kg (≈ 1/4 LD₅₀) and intraperitoneally showed a significant increase in body weight of the rats treated at the 4th week. Animals treated and sacrificed after 30 days showed a disturbance of the relative mass of the organs. Biochemical parameters of hepatic function assessment showed a significant increase in PAL with elevation of AST and ALT, whereas those of renal function revealed a significant decrease in creatinine with an increase in urea. Hematologic parameters recorded a decrease in RBC, HGB and HCT. The histological sections of the treated rats reveal the existence of blood congestion in the central veins and liver tissues, foci of necrosis and steatosis in the liver, blood congestion and some glomerular atrophy in the kidneys, as well as blood congestions and developed follicles without oocytes in the ovaries.

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Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries

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