IGFALS Gene Polymorphisms in Simmental Breed Cattle





SNP, IGFALS, marker, DNA sequencing, Simmental


Defining new genetic markers to understand the population structure and genetic basis of cattle breeds, increase production and improve yield quality is of great importance in the field of modern breeding technology. In this study, it was aimed to identify new molecular marker polymorphisms by using DNA sequence analysis method on the Insulin-Like Growth Factor-Binding Protein, Acıd-Labile Subunit (IGFALS) gene, which affects different yield characteristics in cattle. 20 samples from PCR products with various base sizes were randomly chosen for sequence analysis. The study revealed that the IGFALS gene’s first exon region, which is 492 bp long, contains the g.1368491 A/G polymorphism. A 463 bp length region of the second exon was where the g.1369854 -/G insertion was found.


Akbar HTM, Grala M, Vailati Riboni FC, Cardoso G, Verkerk J, McGowan K, Macdonald J, Webster K, Schutz S, Meier L, Matthews JR and Loor JJ. 2015. Body condition score at calving affects systemic and hepatic transcriptome indicators of inflammation and nutrient metabolism in grazing dairy cows. J. Dairy Sci. 98:1019–1031. http://dx.doi.org/ 10.3168/jds.2014-8584

Alizadeh F, Moradian F and Farhadi A. 2020. Association of allelic polymorphisms of IGFALS gene with growth traits in Makouei and Ghezel sheep breeds. Tropical Animal Health and Production, https://doi.org/10.1007/s11250-020-02321-7.

Baxter RC. 1994. Insulin-Like Growth Factor Binding Proteins in the Human Circulation: A Review. Horm Res 42:140–144 DOI:10.1159/000184186

Boisclair YR, Hurst KR, Ueki I, Tremblay ML, Ooi GT. 2000b. Regulation and role of the acid-labile subunit of the 150-kilodalton insulin-like growth factor complex in the Mouse. Pediatr Nephrol 14:562–566

Boisclair YR, Seto D, Hsieh S, Hurst KR, Ooi GT. 2000a. Organization and chromosomal localization of the gene encoding the mouse acid labile subunit of the insulin-like growth factor binding complex. Proc. Nat. Acad. Sci. 93: 10028-10033

Castilla-Cortazar I, DeIta JR, Aguirre GA, García–Magarino M, Martín-Estal I, Lara-Diaz VJ, Elizondo MI. 2017. Growth hormone insensitivity: Mexican case report. Endocrinology, diabetes & metabolism case reports, https://doi.org/ 10.1530/EDM-17-0126.

Domene HM, Hwa V, Jasper HG, Rosenfeld RG. 2011. Acid-labile subunit (ALS) deficiency. Best Pract. Res. Clin. Endocr. Metab. 25: 101-113

Fang ZH and Pausch H. 2019. Multi-trait meta-analyses reveal 25 quantitative trait loci for economically important traits in Brown Swiss cattle. BMC Genomics 20, sy 1: 695. https://doi.org/10.1186/s12864-019-6066-6.

Flannery BM, Amuzie CJ, Pestka JJ. 2013. Evaluation of Insulin-like Growth Factor Acid-Labile Subunit as a Potential Biomarker of Effect for Deoxynivalenol-Induced Proinflammatory Cytokine Expression. Toxicology 304:192-98. https://doi.org/10.1016/j.tox.2012.12.017.

Gauvin MC, Pillai SM, Reed SA, Stevens JR, Hoffman ML, Jones AK, Zinn SA, Govoni KE. 2020. “Poor Maternal Nutrition during Gestation in Sheep Alters Prenatal Muscle Growth and Development in Offspring”. Journal of Animal Science 98 (1): skz388. https://doi.org/10.1093/jas/skz388.

Guo, Jiazhong, Hossein Jorjani, ve Örjan Carlborg. “A Genome-Wide Association Study Using International Breeding-Evaluation Data Identifies Major Loci Affecting Production Traits and Stature in the Brown Swiss Cattle Breed”. BMC Genetics 13, sy 1: 82. https://doi.org/10.1186/1471-2156-13-82.

Hall T. 2011. Biosciences, I., & Carlsbad, C.J.G.B.B. BioEdit: an important software for molecular biology. GERF Bull Biosci, 2.1: 60-61.

Hector J. 2016. Past, Present, and Future in the Relationship between Growth Retardation and the IGF System: Excerpts from the Cesar Bergada Lecture Given during the SLEP 2015 Annual Meeting. Hormone Research in Paediatrics 86, sy 5: 291-99. https://doi.org/10.1159/000449287.

Hess O, Khayat M, Hwa V, Heath KE, Teitler A, Hritan Y, Tenenbaum‐Rakover Y. 2013. A Novel Mutation in IGFALS, c.380T>C (p.L127P), Associated with Short Stature, Delayed Puberty, Osteopenia and Hyperinsulinaemia in Two Siblings: Insights into the Roles of Insulin Growth Factor-1 (IGF1)”. Clinical Endocrinology 79, sy 6 (Aralık 2013): 838-44. https://doi.org/10.1111/cen.12200.

Hetz JA,. Menzies BR, Shaw G, Rao A, Clarke IJ, Renfree MB.2015. “Growth Axis Maturation Is Linked to Nutrition, Growth and Developmental Rate”. Molecular and Cellular Endocrinology 411: 38-48. https://doi.org/10.1016/ j.mce.2015.04.009.

Högler W, Martin DD, Crabtree N, Nightingale P, Tomlinson J, Metherell L, Frystyk J. 2014. IGFALS Gene Dosage Effects on Serum IGF-I and Glucose Metabolism, Body Composition, Bone Growth in Length and Width, and the Pharmacokinetics of Recombinant Human IGF-I Administration”. The Journal of Clinical Endocrinology & Metabolism 99, sy 4: E703-12. https://doi.org/10.1210/ jc.2013-3718.

Hu ZH, Park CA and Reecy JM. 2019. Building a livestock genetic and genomic information knowledgebase through integrative developments of Animal QTLdb and CorrDB. Nucleic Acids Research, Volume 47, Issue D1, Pages D701–D710. DOI: 10.1093/nar/gky1084

Iniguez G, Argandona F, Medina P, González C, San Martin S. 2011. IGF-IR Signal Transduction Protein Content and Its Activation by IGF-I in Human Placentas: Relationship with Gestational Age and Birth Weight. Editör Ana Claudia Zenclussen. PLoS ONE 9, sy 7: e102252. https://doi.org/10.1371/journal.pone.0102252

Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. Jul;33(7):1870-4, doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.

Lai YC, Li HY, Wu TJ, Jeng CY, Chuang LM. 2014. Correlation of Circulating Acid-Labile Subunit Levels with Insulin Sensitivity and Serum LDL Cholesterol in Patients with Type 2 Diabetes: Findings from a Prospective Study with Rosiglitazone. PPAR Research 2014: 1-10. https://doi.org/10.1155/2014/917823.

Liu Y, Duan X, Liu X, Guo J, Wang H, Li Z, Yang J. 2014. Genetic Variations in Insulin-like Growth Factor Binding Protein Acid Labile Subunit Gene Associated with Growth Traits in Beef Cattle (Bos Taurus) in China. Gene 540, sy 2: 246-50. https://doi.org/10.1016/j.gene.2014.01.044.

Ozdemir M, Sonmez Z, Aksakal V. 2021. Associations between PRL/RsaI polymorphism and some performance traits in Holstein cattle reared under organic condition. J Anim Plant Sci, 31 (3): 900-905, 2021. DOI: 10.36899/ JAPS. 3.0279

McCarthy SD, Waters SM, Kenny DA, Diskin MG, Fitzpatrick R, Patton J, Wathes DC, Morris DG, 2010. “Negative Energy Balance and Hepatic Gene Expression Patterns in High-Yielding Dairy Cows during the Early Postpartum Period: A Global Approach”. Physiological Genomics 42A (3): 188-99. https://doi.org/10.1152/physiolgenomics.00118.2010.

Pareek CS, Sachajko M, Jaskowski JM, Herudzinska M, Skowronski M, Domagalski K, Kumar D. 2019. Comparative Analysis of the Liver Transcriptome among Cattle Breeds Using RNA-Seq. Veterinary Sciences 6, sy 2: 36. https://doi.org/10.3390/vetsci6020036.

Ramirez L, Sanguineti N, Scaglia P, Keselman A, Ballerini MG, Karabatas L, Gutiérrez M. 2020. A Novel Heterozygous STAT5B Variant in a Patient with Short Stature and Partial Growth Hormone Insensitivity (GHI). Growth Hormone & IGF Research 50: 61-70. https://doi.org/ 10.1016 /j.ghir.2019.12.005.

Rechler MM 1993. Insulin-like growth factor binding proteins. Vitam Horm 47:1–114

Sönmez Z. 2017. Sığırlarda verim özelliklerini etkileyen bazı moleküler markır polimorfizmlerinin belirlenmesi ve verimle ilişkilendirilmesi, Atatürk Üni. Fen Bil. Ens. Doktora Tez, ERZURUM.

Storr HL, Prasad R, Temple IK, Metherel LA, Savage MO, Walker JM. 2015. Heterogeneity of the Growth Phenotype and Birth Size in Acid-Labile Subunit (ALS) Deficiency. Journal of Endocrinological Investigation 38, sy 4: 407-12. https://doi.org/10.1007/s40618-014-0195-1.

Ueki I, Giesy SL, Harvatine KJ, Kim JW and Boisclair YR. 2009. The acid-labile subunit is required for full effects of exogenous growth hormone on growth and carbohydrate metabolism. Endocrinology.;150(7):3145-52. DOI: 10.1210/en.2008-1740.

Wathes DC, Cheng Z, Fenwick MA, Fitzpatrick R, Patton J, 2011. Influence of Energy Balance on the Somatotrophic Axis and Matrix Metalloproteinase Expression in the Endometrium of the Postpartum Dairy Cow. REPRODUCTION 141, sy 2: 269-81. https://doi.org/10.1530/REP-10-0177.

Yeh, F. C., Yang, R. C., & Boyle, T. 1999. PopGene: Microsoft Window-based freeware for population genetic analysis, version 1.31. University of Alberta: Edmonton, Canada.

Zhi-Liang Hu Carissa AP and James MR. 2019. Building a livestock genetic and genomic information knowledgebase through integrative developments of Animal QTLdb and CorrDB. Nucleic Acids Research, Volume 47, Issue D1, 8 January 2019, Pages D701–D710. DOI: 10.1093/nar/gky1084




How to Cite

Genç, R., & Sönmez, Z. (2023). IGFALS Gene Polymorphisms in Simmental Breed Cattle. Turkish Journal of Agriculture - Food Science and Technology, 11(4), 846–850. https://doi.org/10.24925/turjaf.v11i4.846-850.5873



Research Paper