The Effect of Selenium, Vitamin E, Vitamin A and Vitamin D3 Applications on Fertility in Awassi Sheep with Estrus Synchronization During the Breeding Season

Authors

DOI:

https://doi.org/10.24925/turjaf.v11i8.1331-1336.5946

Keywords:

Sheep, synchronization, selenium, vitamin E, vitamin A, vitamin D3

Abstract

The purpose of this study was to determine the effects of selenium, vitamin E, vitamin A, and vitamin D3 treatments on reproductive parameters in Awassi sheep fed solely dry grass and grain stubble during the breeding season with estrus synchronization. Seventy-five sheep were implanted with intravaginal sponges containing 20 mg of flugestone acetate for 9 days for estrus synchronization. On the day the sponges were inserted, the first group received an intramuscular injection of a supplement containing 200,000 IU of vitamin A, 30,000 IU of vitamin D3, and 20 mg of vitamin E, as well as a supplement containing 1 mg of sodium selenite and 60 mg of vitamin E. The second group was the control group, with no supplementary vitamin injected. On the day of sponge removal, the sheep received intramuscular injections of 500 IU PMSG and 250 mcg cloprostenol sodium in both groups. The sheep in the first group were given a second injection of the supplement on the same day that contained 20 mg of vitamin E, 30,000 IU of vitamin D3, and 200,000 IU of vitamin A. The sheep that showed signs of estrus were mated naturally after the estrus synchronization. Estrus rates were 86.11% and 85.29%, conception rates were 74.19% and 58.62%, pregnancy rates were 63.88% and 50%, lambing rates were 100% and 100%, and litter size was found to be 126.08% and 123.52% in Group I and II, respectively (P=0.858). Although there was no significant difference in the reproductive characteristics between the groups (P>0.05), Group I had a greater pregnancy rate and litter size. During the breeding season, it is believed that the regular application of vitamin and mineral supplements on a program basis in sheep fed only dry pasture and grain stubble contributed to fertility.

References

Abdelrahman MM, Al-Karablieh EK. 2002. Effect of Vitamins AD3E Injections on Reproductive Performance and Net Cash Revenue from Awassi Sheep Raised under Semi-intensive System. J King Saud Univer, 14: 15-22.

Agarwal A, Allamaneni SS. 2004. Role of free radicals in female reproductive diseases and assisted reproduction. Reprod Biomed Online, 9(3): 338-347

Agarwal A, Gupta S, Sharma RK. 2005. Role of oxidative stress in female reproduction. Reprod Biol Endocrin, 3(1): 1-21.

Alaçam E. 1993. Koyunlarda siklik düzen ve üremenin denetlenmesi. Hayvancılık Araş Derg, 3: 65-69.

Alaçam E. 1999. Üreme Kontrolü. In: Alaçam E (editör). Evcil Hayvanlarda Doğum ve İnfertilite, Medisan, Ankara, pp. 71-81.

Albertini D, Barrett S. 2002. Oocyte-somatic cell communication. Reprod Suppl, 61: 49-54.

Arechiga CF, Vazquez-Flores S, Ortiz O, Hern J, Porras A, McDowell LR, Hansen PJ. 1998. Efect of injection of β-carotene or Vitamin E and selenium on fertility of lactating dairy cows. Theriogenology, 50: 65-76.

Awawdeh MS, Eljarah AH, Ababneh MM. 2019. Multiple injections of vitamin E and selenium improved the reproductive performance of estrus-synchronized Awassi ewes. Trop Anim Health Prod, 51(6): 1421-1426.

Bagavandoss P, Midgley AR. 1988. Biphasic action of retinoids on gonadotropin receptor induction in rat granulosa cells in vitro. Life Sci, 43(20): 1607-1614.

Bindari YR, Shrestha S, Shrestha N, Gaire TN. 2013. Effects of nutrition on reproduction-A review. Adv Appl Sci Res, 4(1): 421-429

Birdane MK, Avdatek F. 2020. Effect of vitamin A, D3, E treatment on fertility in the Pırlak sheep. Kocatepe Vet J, 13(2): 179-184.

Boland TM, Brophy PO, Callan JJ, Quinn PJ, Nowakowski P, Crosby TF. 2005. The effects of mineral supplementation to ewes in late pregnancy on colostrum yield and immunoglobulin G absorption in their lambs. Livest Prod Sci, 97(2-3): 141-150.

Bozkurt T, Gür S, Sönmez M. 1998. Vitamin AD3E'nin ineklerin döl verimi üzerine etkisi. YYÜ Vet Fak Derg, 9(1): 80-82.

Braun U, Forrer WF, Lutz H. 1991. Selenium and vitamin E in blood sera of cows from farms with increased incidence of disease. Vet Rec, 128: 543-547.

Brown JA, Eberhardt DM, Schrick FN, Roberts MP, Godkin JD. 2003. Expression of retinol-binding protein and cellular retinol binding protein in the bovine ovary. Mol Reprod Dev, 64: 261-269.

Ceko MJ, Hummitzsch K, Hatzirodos N, Bonner WM, Aitken JB, Russell DL, Lane M, Rodgers RJ, Harris HH. 2014. X-Ray fluorescence imaging and other analyses identify selenium and GPX1 as important in female reproductive function. Metallomics, 7(1): 71-82.

Chew BP. 1993. Effects of supplemental β-carotene and vitamin A on reproduction in swine. J Anim Sci, 71(1): 247-252.

Eberhardt DM, Will WA, Godkin JD. 1999. Retinol administration to superovulated ewes improves in vitro embryonic viability. Biol Reprod, 60(6): 1483-1487.

Farahavar A, Rostami Z, Alipour D, Ahmadi A. 2020. The effect of pre-breeding vitamin E and selenium injection on reproductive performance, antioxidant status, and progesterone concentration in estrussynchronized Mehraban ewes. Trop Anim Health Prod, 52(4): 1779-1786.

Gordon I. 1997. Controlled Reproduction in Sheep and Goats, Universty College Dublin, Ireland, pp. 241-259.

Haliloğlu S, Baspjnar N, Serpek B, Erdem H, Bulut Z. 2002. Vitamin A and β -carotene levels in plasma, corpus luteum and follicular fluid of cyclic and pregnant cattle. Reprod Dom Anim, 37: 96-99.

Harb M. 1999. Sheep production under extensive systems in the Near East: Jordan pastoral system, a case study. FAO/RNE Near East Regional Office, Food and Agriculture Organization of the United Nations: Rome, Italy.

Hashem NM, Abd-Elrazek D, Abo-Elezz ZR, Latif MGA. 2016. Effect of vitamin A or C on physiological and reproductive response of Rahmani ewes during subtropical summer breeding season. Small Rumin Res, 144: 313-319.

Hemingway RG. 2003. The influences of dietary intakes and supplementation with selenium and vitamin E on reproduction diseases and reproductive efficiency in cattle and sheep. Vet Res Commun, 27: 159-174.

Horst RL, Goff JP, Renhardt TA. 2003. Role of vitamin D in calcium homeostasis and its use in prevention of bovine periparturient paresis. Acta Vet Scan Supp, 97: 35-50.

Hostetler CE, Kincaid RL, Mirando MA. 2003. The role of essential trace elements in embryonic and fetal development in livestock. Vet J, 166 (2): 125-139.

Humann-Ziehank E. 2016. Selenium, copper and iron in veterinary medicine-From clinical implications to scientific models. J Trace Elements Med Biol, 37: 96-103.

Infante JP. 1999. A function for the vitamin E metabolite α-tocopherol quinone as an essential enzyme cofactor for the mitochondrial fatty acid desaturases. FEBS Letters, 446: 1-5.

Jozwik M, Wolczynski S, Szamatowicz M. 1999. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Reprod, 5: 409-413.

Kaçar C, Kamiloğlu NN, Uçar Ö, Arı UÇ, Pancarcı ŞM, Güngör Ö. 2008. İneklerde β-karoten+E Vitamini uygulamasıyla kombine edilen ovsynch ve cosynch senkronizasyon programlarının gebelik oranı üzerine etkisi. Kafkas Üniv Vet Fak Derg, 14(1): 45-50.

Kolb E, Seehawer J. 1998. Nutritional biochemical aspects of the use of beta-carotene, vitamin A, D and E as well as ascorbic acid in domestic animals and influence on the secretion and activity of hormones. Tierarztl Umsch, 63: 150-156.

Kott RW, Thomas VM, Hatfield PG, Evans T, Davis KC. 1998. Effects of dietary vitamin E supplementation during late pregnancy on lamb mortality and ewe productivity. J Am Vet Med Assoc, 212 (7): 997-1000.

Koyuncu M, Yerlikaya H. 2007. Effect of selenium-vitamin E injections of ewes on reproduction and growth of their lambs. S Afr J Anim Sci, 37: 233-236.

Köse M, Kırbaş M, Dursun Ş, Bayrıl T. 2013. Anöstrüs döneminde koyunlara β-karoten veya E vitamini+ selenyum enjeksiyonlarının döl verimi üzerine etkisi. YYÜ Vet Fak Derg, 24(2): 83-86.

Kuru M, Sogukpinar O, Makav M, Cetin N. 2017. Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season. Med Weter, 73(8): 479-82.

Liu S, Masters D, Ferguson M, Thompson A. 2014. Vitamin E status and reproduction in sheep: potential implications for Australian sheep production. Anim Prod Sci, 54(6): 694-714.

Mehdi Y, Dufrasne I. 2016. Selenium in cattle: a review. Molecules, 21(4): 545.

Miyamoto K, Sato EF, Kasahara E, Jikumaru M, Hiramoto K, Tabata H, Katsuragia M, Odob S, Utsumic K, Inouea M. 2010. Effect of Oxidative Stress During Repeated Ovulation on the Structure and Functions of the Ovary, Oocytes, and Their Mitochondria. Free Radic Biol Med, 49: 674-681.

Munoz C, Carson AF, McCoy MA, Dawson LER, O’Connell NE, Gordon AW. 2008. Nutritional status of adult ewes during early and mid-pregnancy. 1. Effects of plane of nutrition on ewe reproduction and offspring performance to weaning. Animal, 2(1): 52-63.

Muscogiuri G, Altieri B, Angelis C, Palomba S, Pivonello R, Colao A, Orio F. 2017. Shedding new light on female fertility: The role of vitamin D. Rev Endocr Metab Disord,18(3): 273-283.

Nayyar S, Jindal R. 2010. Essentiality of antioxidant vitamins for ruminants in relation to stress and reproduction. Iranian J Vet Res, 11: 1-9.

Nebbia C. 1982. Selenium in veterinary medicine. Riv Zoot Vet, 10: 246-278.

Özar E, Sarıbay MK, Köse AM, Sertkol R. 2022. Effects of selenium, vitamin E, and β-carotene administration on fertility of Awassi ewes synchronized for estrus in non-breeding season. MAE Vet Fak Derg, 7 (3): 167-174.

Pu Y, Wang Z, Bian Y, Zhang F, Yang P, Li Y, Zhang Y, Liu Y, Fang F, Cao H, Zhang X. 2014. All-trans retinoic acid improves goat oocyte nuclear maturation and reduces apoptotic cumulus cells during in vitro maturation. Anim Sci J, 85: 833-839.

Sen W, Fucai C, Yanyan W, Zhanqin Z. 2011. Effects of selenium supplement on the serum hormone concentration and super ovulation effect of Suffolk sheep. Chinese Agric Sci Bull, 23: 9-23.

Taniguchi K, Taketani T, Lee LM, Kizuka F, Tamura I, Sugino N. 2009. Melatonin protects granulosa cells for progesterone production as an antioxidant in human ovarian follicles. Biol Reprod, 81: 378-378.

Turan ÖD. 2018. Vitamin D Level and Infertility. Meandros Med Dent J, 19(2): 106.

White CL, Rewell L. 2007. Vitamin E and selenium status of sheep during autumn in Western Australia and its relationship to the incidence of apparent white muscle disease. Aust J Exp Agric, 47: 535-543.

Wildeus S. 2000. Current concepts in synchronization of estrus: Sheep and goats. J Anim Sci, 77: 1- 14.

Yeşil M, Sarıözkan S. 2017. Dişi üreme sistemi açısından önemli bazı vitamin ve mineraller. Erciyes Üniv Vet Fak Derg, 14(3): 201-208.

Downloads

Published

31.08.2023

How to Cite

Efe, M., Sarıbay, M. K., Koldaş Ürer, E., & Köse, A. M. (2023). The Effect of Selenium, Vitamin E, Vitamin A and Vitamin D3 Applications on Fertility in Awassi Sheep with Estrus Synchronization During the Breeding Season . Turkish Journal of Agriculture - Food Science and Technology, 11(8), 1331–1336. https://doi.org/10.24925/turjaf.v11i8.1331-1336.5946

Issue

Section

Research Paper