Effects of Inoculation of Lactobacillus plantarum at Different Doses on Triticale (Triticosecale wittmack) Silage on Quality, Fermentation and Aerobic Stability Properties and Feed Value

Authors

DOI:

https://doi.org/10.24925/turjaf.v12i2.201-207.6323

Keywords:

triticale silage, Lactobacillus plantarum, silage quality, aerobic stability, feed value

Abstract

This study was conducted to determine the effects of different doses of Lactobacillus plantarum (LP) inoculation into triticale silage on fermentation, quality, feed value, and aerobic stability. This study used three doses of LP bacteria strains (MF098786 strain) isolated from homemade pickles as inoculants. As LP dose, 1×106, 1×108 and 1×109 cfu/mL levels were used. The LP inoculation was applied by spraying onto by using a sterile injector at 1 mL per 1 kg material. The prepared silages were incubated for 60 d. The treatment groups in the study consisted of triticale control (TC), 1×106 (LP6T), 1×108 (LP8T) and 1×109 cfu/kg DM (LP9T) LP inoculated triticale. The LP inoculation of triticale silage improved silage fermentation, chemical and microbiological properties, silage quality, and feed value, and aerobic stability of the product, regardless of dose application. This application did not change the silage's organic matter, ash, and hemicellulose contents but decreased the crude fiber, neutral detergent fiber, and acid detergent fiber contents. While there was no significant change in color parameters in all silages, a decrease in the ultimate pH value, and improvement in Flieg score and RFV were detected. The LP inoculation into triticale silage increased the number of lactic acid bacteria and decreased the number of yeast in the silages. This application improved the total digestible nutrient and energy values of LP9T silage compared with other silages. When LP doses were evaluated within themselves, it was determined that all doses gave almost similar results in terms of the parameters studied. However, when the data obtained from the research are evaluated as a whole, LP inoculation at the level of 1×109 cfu/mL can be recommended to triticale silage, because of the positive effects of silage on total digestible nutrient, digestible energy, metabolizable energy, and net energy contents.

References

Addah, W. (2022). Microbial approach to improving aerobic stability of silage. Nigerian Journal of Animal Science, 24(2), 231-244. https://www.ajol.info/index.php/tjas/article/view/233751/220781 [Accessed 26 July 2023].

AMSA. (2012). Meat color measurement guidelines. The American Meat Science Association. https://meatscience.org/docs/default-source/publications-resources/hot-topics/download-the-ebook-format-pdf-of-the-meat-color-measurement-guidelines.pdf?sfvrsn=a218b8b3 [Accessed 30 November 2021].

AOAC. (2000). Official methods of analysis, 17th edition. AOAC International, Gaithersburg, Maryland, USA.

AOCS. (2005). Official procedure. approved procedure am 5–04, rapid determination of oil/fat utilizing high temperature solvent extraction. Urbana, IL: American Oil Chemists’ Society.

Ashbell, G., Weinberg, Z., Azrieli, A., Hen, Y., & Horev, B. (1991). A simple system to study the aerobic deterioration of silages. Canadian Agricultural Engineering, 33, 391–394. https://library.csbe-scgab.ca/docs/journal/33/33_2_391_ocr.pdf [Accessed 02 December 2021]

Ávila, C. L. S., & Carvalho, B. F. (2020). Silage fermentation—updates focusing on the performance of micro‐organisms. Journal of Applied Microbiology, 128(4), 966-984. https://doi.org/10.1111/jam.14450

Carvalho, B. F., Sales, G. F. C., Schwan, R. F., & Ávila, C. L. S. (2021). Criteria for lactic acid bacteria screening to enhance silage quality. Journal of Applied Microbiology, 130(2), 341–355. https://doi.org/10.1111/jam.14833

Çayıroğlu, H., Filik, G., Coşkun, İ., Filik, A. G., Çayan, H., & Şahin, A. (2020). Spraying opened sugar beet pulp silage with oregano essential oil helps to sustain quality and stability. South African Journal of Animal Science, 50(1), 9-16. htpp://doi.org/10.4314/sajas.v50i1.2

Erdem, B., Kıray, E., Kariptaş, E., Tulumoğlu, Ş., & Akıllı, A. (2021). Characterization of probiotic abilities of lactic acid bacteria from traditional pickle juice and shalgam. Research and Reviews in Science and Mathematics, 1(33). ttps://www.researchgate.net/profile/Enihal-Ercan/publication/352178517_Imtiyaz_Sahibi_Publisher_Yasar_Hiz/links/60bda14992851cb13d83f470/Imtiyaz-Sahibi-Publisher-Yasar-Hiz.pdf [Accessed 22 June 2023].

González-Alcántara, F. D. J., Estrada-Flores, J. G., Morales-Almaraz, E., López-González, F., Gómez-Miranda, A., Vega-García, J. I., & Arriaga-Jordán, C. M. (2020). Whole-crop triticale silage for dairy cows grazing perennial ryegrass (lolium perenne) or tall fescue (lolium arundinaceum) pastures in small-scale dairy systems during the dry season in the highlands of Mexico. Tropical Animal Health and Production, 52, 1903-1910. htpp://doi.org/10.1007/s11250-020-02206-9

Harper, M. T., Oh, J., Giallongo, F., Roth, G. W., & Hristov, A. N. (2017). Inclusion of wheat and triticale silage in the diet of lactating dairy cows. Journal of Dairy Science, 100(8), 6151-6163. htpp://doi.org/10.3168/jds.2017-12553

Huuskonen, A., Jaakkola, S., & Manni, K. (2020). Intake, gain and carcass traits of Hereford and Charolaise bulls offered diets based on triticale, barley and grass silages. Agricultural and Food Science, 29(4), 318–330. https://doi.org/10.23986/afsci.89813

İnce, A., & Vurarak, Y. (2019). An Approach to color change and quality relation in roughages. Journal of Agricultural Sciences, 25(1), 21-28. https://doi.org/10.15832/ankutbd.538982

Jeranyama, P., & Garcia, A. D. (2004). Understanding relative feed value (RFV) and relative forage quality (RFQ). SDSU Extension Extra Archives, 352. https://openprairie.sdstate.edu/extension_extra/352/ [Accessed 30 November 2021].

Jung, J. S., Ravindran, B., Soundharrajan, I., Awasthi, M. K., & Choi, K. C. (2022). Improved performance and microbial community dynamics in anaerobic fermentation of triticale silages at different stages. Bioresource Technology, 345: 126485. https://doi.org/10.1016/j.biortech.2021.126485

Kılıç, A. (2006). Determined of quality in roughage. Hasat Publication, İstanbul. 68-69. (In Turkish: Kaba yemlerde niteliğin saptanması, Hasat Yayıncılık, İstanbul. 68–69.

Kılınç, K., & Gökkuş, A. (2022). Effects of different harvesting times on yield and quality of oat and triticale [In Turkish: Farklı biçim zamanlarının yulaf ve tritikale otunun verim ve kalitesine etkileri]. Acta Natura et Scientia, 3(2), 108-115. https://doi.org/10.29329/actanatsci.2022.352.03

Kim, D. H., Lee, K. D., & Choi, K. C. (2021). Role of LAB in silage fermentation: effect on nutritional quality and organic acid production—An overview. AIMS Agriculture and Food, 6(1): 216-234. https://doi.org/10.3934/agrfood.2021014

Ma, J., Dai, H., Liu, H., & Du, W. (2022). Effects of cutting stages and additives on the fermentation quality of triticale, rye and oat silage in qinghai-Tibet Plateau. Agronomy, 12(12), 3113. https://doi.org/10.3390/agronomy12123113

NRC. (2001). Nutrient requirements of dairy cattle: 7th revised ed., 405. https://doi.org/10.17226/9825

O’Kiely, P. (2011). Intake, growth and feed conversion efficiency of finishing beef cattle offered diets based on triticale, maize or grass silages, or ad libitum concentrate. Irish Journal of Agricultural and Food Research, 50, 189–207. https://www.jstor.org/stable/41549251 [Accessed 26 July 2023].

Oladosu, Y., Rafii, M. Y., Abdullah, N., Magaji, U., Hussin, G., Ramli, A., & Miah, G. (2016). Fermentation quality and additives: A case of rice straw silage. BioMed Research International, 7985167. https://doi.org/10.1155/2016/7985167

Oliveira, J. F. A., Jakelaitis, A., Cabral Filho, S. L. S., Silva, C. J. D., Guimarães, K. C., Pereira, L. S., Sousa, G. D. & Oliveira, G. S. D. (2021). Silage quality from ıntercropping corn and soybean managed with ınoculant azospirillum brasilense and nitrogen fertilization. Revista Brasileira de Saúde e Produção Animal, 22, 1–15. https://doi.org/10.1590/S1519-99402122092021

Ozduven, M. L., Onal, Z. K., & Koc, F. (2010). The Effects of bacterial inoculants and/or enzymes on the fermentation, aerobic stability and in vitro dry and organic matter digestibility characteristics of triticale silages. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(5), 751-756. https://doi.org/10.9775/kvfd.2009.1628

Sahar, A. K., Vurarak, Y., Cubukcu, P., & Oluk, C. A. (2022). Effects of storage length and variety on some quality and color parameters in soybean silage. Journal of Elementology, 27(4). http://dx.doi.org/10.5601/jelem.2022.27.4.2291

Seale, D. R., Pahlow, G., Spoelstra, S. F., Lindgren, S., Dellaglio, F., & Lowe, J. F. (1990). Methods for the microbiological analysis of silage. Proceeding of the Eurobac Conference, 147, Uppsala.

Singh, D., Chauhan, A., & Chaudhary, A. (2020). Evaluation of maize cultivars for forage yield, silage quality traits and nutrient uptake in agro-climatic conditions of Central Gujarat, India. Range Management and Agroforestry, 41(1), 133–140. https://www.researchgate.net/profile/Digvijay-Singh-8/publication/343820492 [Accessed 30 November 2021].

Soundharrajan, I., Park, H. S., Rengasamy, S., Sivanesan, R., & Choi, K. C. (2021). Application and future prospective of lactic acid bacteria as natural additives for silage production—A review. Applied Sciences, 11(17), 8127. http://dx.doi.org/10.3390/app11178127

Sucu, E., & Çifci, E. A. (2016). Effects of lines and inoculants on nutritive value and production costs of triticale silages. Revista Brasileira de Zootecnia, 45, 355-364. http://dx.doi.org/10.1590/S1806-92902016000700001

Turan, A., & Önenç, S. S. (2018). Effect of cumin essential oil usage on fermentation quality, aerobic stability and in vitro digetibility of alfalfa silage. Asian-Australas Journal Animal Science, 31(8), 1252-1258. https://doi.org/10.5713%2Fajas.17.0834

Van Soest, P. J., Robertson, J. D., & Lewis, B. A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. Journal Dairy Science, 74:10, 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Wilkinson, J. M., & Rinne, M. (2018). Highlights of progress in silage conservation and future perspectives. Grass and Forage Science, 73(1), 40-52. https://doi.org/10.1111/gfs.12327

Downloads

Published

25.02.2024

How to Cite

Çayıroğlu, H. (2024). Effects of Inoculation of Lactobacillus plantarum at Different Doses on Triticale (Triticosecale wittmack) Silage on Quality, Fermentation and Aerobic Stability Properties and Feed Value. Turkish Journal of Agriculture - Food Science and Technology, 12(2), 201–207. https://doi.org/10.24925/turjaf.v12i2.201-207.6323

Issue

Section

Research Paper