Haematological Indices and Fertility Potential of Rabbits Receiving Camels Foot (Piliostigma thonningii) Essential Oil Supplemented Diet
DOI:
https://doi.org/10.24925/turjaf.v12i12.2470-2477.6870Keywords:
bucks, tropical phytogenics, feed supplement, blood, semenAbstract
The current study aimed to evaluate Piliostigma thonningii seeds-derived essential oil (PTO) effect on hematological and reproductive parameters in rabbits. Three groups consisting of 15 animals each were randomly assigned and with an average initial body weight (BW) of 262.89 ± 22.36 g in a fully randomised experimental design. Group 1 received the control diet, while for groups 2 and 3 the basal control diet was supplemented with 2 mL PTO/kg diet and 4 mL PTO/kg diet, respectively. At the end of the experiment, blood samples were collected and the blood was analysed using the ABACUS ROSS haematology analyser. The results indicated significant differences in rabbits receiving PTO supplemented feed, namely; Packed cell volume, red blood cells, white blood cells, mean corpuscular haemoglobin concentration, neutrophil increased (P<0.05) with increasing level of PEO supplementation. Mean corpuscular volume and haemoglobin, lymphocyte, monocyte and platelet were higher (P<0.05) in T2 and T3 than in T1. Neutrophil/lymphocyte ratio was higher (P<0.05) in T3 than T1 with T2 being intermediate (P>0.05) between T1 and T3. Semen volume, concentration and motility were higher (P<0.05) in T2 and T3 than in T1. While semen abnormalities and bucks’ reaction time to does (libido) were greater (P<0.05) in T1 than in T2 and T3, Live dead ratio was higher (P<0.05) in T3 relative to T1 while T2 was intermediate between T1 and T3 (P>0.05). semen color and pH were not affected (P>0.05) by treatments. It was therefore concluded that P. thonningii essential oil supplementation enhanced both haematological and fertility potential of the experimental rabbits.
References
Abdel-Wareth, A.A.A. and Metwally, A.E. (2020). Effects of dietary phytogenic supplementation on performance, semen quality and serum biochemical of rabbits. https://doi.org/10.21203/rs.3.rs-28978/v1.
Ajiboye, A.T., Lawal, S.O., Otun, K.O., Bale, A.T. and Arowon, M.T. (2017). Evaluation of physico-chemical and antimicrobial studies of Delonix regia and Piliostigma thonningii seed oils. Jordan Journal of Chemistry 12(1): 21-32.
Anaso EU, Olafadehan OA, Chibuogwu IC, Alagbe JO. Seminal mophology and organ morphometrics of rabbit bucks fed Piliostigma thinningii essential oil supplementd diet. Science Letters 2024; 12(2):70-75.
Anaso EU, Olafadehan. OA and Chibuogwu, IC. Semen characteristics of rabbits fed Camel’s foot (Piliostigma thonningii) essential oil supplemented diet. Discovery 2023; 59: e36d1037.
Anaso EU. Bioactive compounds of piliostigma thonningii essential oil detected by the gas chromatography-mass spectrometry. EJABS, 2023b: 3(2): 68-72.
Anaso EU. Phytogenics and essential oil supplementation in rabbit and monogastrate production, a panacea to improved animal production and antibiotic resistance challenge in Nigeria. EJABS, 2023a: 3(2):45-51.
Anaso, E.U., Olafadehan, O.A. and Oluwafemi, R.A. (2021). Biodegradation of agro industrial by products as a panacea to feed scarcity in Nigeria amidst farmer insecurity and global pandemic. Proceedings of the 46th Annual Nigerian Society for Animal Production Conference, Dutsin-Ma, Nigeria, pp. 616 - 620.
Angel-Gracia, O., Meza- Herrera, C.A., Guillen-Munoz, J.M., Carillo-Castellanos, E., Luna- Orozko, J.R., Mellado, M. and Veliz- Deraz, F.G. (2015). Seminal characteristics, libido and serum testosterone concentrations in mixed –breed goat bucks receiving testosterone during the non-breeding period. Journal of Applied Animal Research 43(4): 457-461.
Ayoade, J.A., Wuanor, A.A. and Ochepo, G.O. (2015). Performance, haematology and serum biochemistry of grazing Bunaji bulls supplemented varying levels an agro industrial by- product based diet. IOSR Journal of Agriculture and Veterinary Science 8: 95-100.
Bahare, S., Upadhyay, I., Erdogan, O., Arun Kumar, J, Sumali, L.D.J., Daniel A.D., Farukh, S., Yasaman, T., Natália, M., Navid, B., William, C. and Cho, J.S. (2019). Antidiabetic potential of medicinal plants and their active components. Biomolecules 30;9(10):551. doi: 10.3390/biom9100551.
Bassiony, S.S.M., Elhindawy, M.M., Attia, A.E. and Ismail, I.E. (2015). Effect of some bioactive components of essential oils on growing rabbits performance. Zagazig Journal of Agricultural Research 42(5).
Beers, M.H. (2006). The Merck manual of diagnosis and therapy. 18th ed. Whitehouse Station, N.J. Merck Research Laboratories.
Brian, T. (2009). Red blood cells. Microsoft Encartha (DVD) Redmond, W.A. Microsoft Corporation.
CBRA-Colegio Brasileiro de Reproduçâo (1998). Animal. Manual de exame e avaliação de sêmen animal. 2. ed. Belo Horizonte: CBRA.
Chattopadhyay, B.P., Mahata, A., Kundu, S., Alan, J., Dars, S. and Roychawdhury, A. (2007). A study on blood eosinophil level and ventilatory pulmonary function of the workers exposed to storage grain dust. Indian Journal of Occupational and Environmental Medicine 11(1): 21–25.
Elghalid, O.A., Kholif, A.E., El-Ashry, G.M., Matloup, O.H., Olafadehan, O.A., El-Raffaa, A.M and AbdEl-Hadya, A.M. (2020). Oral supplementation of the diet of growing rabbits with a newly developed mixture of herbal plants and spices enriched with special extracts and essential oils affects their productive performance and immune status. Livestock Science 238: 104082.
El-Gindy, Y.M., Abu Hafsa, S.H. and Dosoky, W.M. (2020) Effects of potato peel extract on semen quality, sex hormones and immunity of rabbit bucks under intensive breeding system. Journal of the American Oil. https://doi.org/10.1111/and.13869
El-Gogary, M.R., El-Said, E.A. and Mansour, A.M. (2018). Physiological and immunological effects of rosemary essential oil in growing rabbit diets. Journal Agricultural Science 10: 485.
El-Ratel, I.T., Attia, K.A.H., El-Raghi, A.A. and Fouda, S.F. (2021). Relief of the negative effects of heat stress on semen quality, reproductive efficiency and oxidative capacity of rabbit bucks using different natural antioxidants. Animal bioscience 34(5): 844-854.
Hancock, J.I. (1951). A staining technique for the study of temperature shock in semen. Nature, London 197: 323-343.
Ismail, I.E. and Bealish, A.M.A. (2014). Effect of acetic acid addition on growth and some physiological and immunological characteristics of growing rabbits. Egyptian Journal of Rabbit Science 24 (1): 19 – 34.
Jain N. C. (1986). Scanning electron micrograph of blood cell. In Schalm’s Veterinary Haematology, D.J. Weiss and K.J. Wardrop Eds. John Willey and Sons Inc. New York Pp. 63-70.
Jelalu, K. (2014). Laboratory manual and review of clinical pathology. OMICS group ebooks, USA. pp. 5-21.
Jimoh, F.O. and Oladiji, A.T. (2005). Preliminary Studies on Piliostigma thonningii Seeds: Proximate Analysis, Mineral Composition and Phytochemical Screening. African Journal of Biotechnology, 4, 1439-1442.
Mahgoub, A.M., Aufy, M.G., Saadi, M.G. and Adel Elmallawany, M. (2009). Risk factors predisposing to Toxoplasmosis in chronic renal patients and renal transplant recipients. Journal of Egyptian Society for Parasitology 36: 945-958.
Mahmoud, M.A., Shehata, S.S.A and Khalil, B.A. (2016). Effect of water thyme extract on growing rabbit’s performance. Zagazig Journal of Agricultural Research 43 (6A): 2023-2035.
Mohamed, A., Ferhet Brahim, Y., Meklati, J.S. and Farid, C. (2006). An improved microwave Clevenger apparatus for distillation of essential oil from orange peel. Journal of chromatography 1112: 121-126.
NRC (1984). Nutrient requirements of poultry. 9th Ed. Nat. Res. Coun., Nat. Acad. Press, Washington, D.C., USA.
Ogbiko C1., Chukwunwejim, C. R. and Okezie, U. (2021). Phytochemical Composition, Acute Toxicity and Lipid Profile of the Methanol Leaf Extract of Piliostigma Thonningii (Fabaceae). Journal of Pharmaceutical and Allied Sciences 18 (1) 3430 – 3436.
Olafadehan O. A. (2011). Haematological Parameters, Serum Constituents and Organ Development of Growing Rabbits as Affected by Feeding of Processed Cassava Peels. Animal Nutrition and Feed Technology Vol. 11(1) Pp 41- 51.
Olafadehan, O.A., Adewumi, M.K. and Okunade, S.A. (2014). Effects of feeding tanning- containing forage in varying proportion with concentrate on the voluntary intake, haematological and biochemical indices of goats. Trakia Journal of Sciences 12: 73- 81.
Osinowo, O.A. (2016). Introduction to Animal Reproduction, Sophie Academics Services Limited. 2nd edition.
Ososanya, T. O., Adewumi, M. K., Oyeyemi, M. O. and Adeosun, A. O. (2013). Semen characteristics of pubertal West Africa dwarf rams fed pineapple waste silage as replacement for dried cassava peel. African Journal of Agricultural Research 9(4): 501- 506.
Oyeyemi. M.O., Leigh, O.O. and Fakayode, M.O. (2011). Spermiogram and changes in body parameters of West African Dwarf bucks fed dussa- poultry waste combination. Global Veterinaria 6(2): 136-139.
Pandi N, Sonawane SH, Anand Kishore K. Synthesis of cellulose nanocrystals (CNCs) from cotton using ultrasound-assisted acid hydrolysis. Ultrason Sonochem. 2021 Jan;70:105353. doi: 10.1016/j.ultsonch.2020.105353. Epub 2020 Sep 19. PMID: 33007536; PMCID: PMC7786604.
Romero, C., P.G. Rebollar, A. Dal Bosco, C. Castellini and R. Cardinali (2011). Dietary effect of short-chain organic acids on growth performance and development of intestinal lymphoid tissues in fattening restricted rabbits. World Rabbit Science 19: 133 – 142.
SCLabs. (2016). Beyond Aroma: Terpenes in cannabis Archived 2016-06-15 at the Wayback Machine.
Zhou, L., Zhao, Y., Wang, S., Han, S and Liu, J., (2015). Lead in the soil–mulberry (Morus alba L.)–silkworm (Bombyx mori) food chain: Translocation and detoxification. Chemosphere, 128: 171–177.
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