Debittering Process of Lupin (Lupinus albus l.) by Ultrasound Pre-treatment

Authors

DOI:

https://doi.org/10.24925/turjaf.v12i10.1673-1678.6921

Keywords:

Alkaloid, bioactive compound, debittering, lupin, ultrasound

Abstract

This study investigates the efficacy of ultrasound as a pre-treatment method for the debittering process of Lupin (Lupinus albus L.), aiming to enhance its overall quality and nutritional profile. Lupin seeds are abundant in protein and other essential nutrients, yet they contain bitter alkaloids, primarily lupinine and lupinidine, which pose challenges in their utilization as food and feed. Traditional methods of debittering involve water soaking, which are time-consuming and may result in nutrient losses. Ultrasound emerges as a promising alternative due to its ability to accelerate the debittering process without compromising nutritional integrity. In this research, Lupin seeds underwent ultrasound pre-treatment under various conditions to optimize debittering efficiency. Parameters such as ultrasound power, treatment temperature  and duration were systematically evaluated to determine their impact on alkaloid removal and preservation of nutritional content. The pre-treated Lupin samples were then analyzed for changes in alkaloid concentrations, protein content, and other nutritional attributes. The applied ultrasound-assisted extraction method brought a new perspective and the heating process was adapted with ultrasound and the bitterness removal process was performed in a shorter time. Preliminary results demonstrate the effectiveness of ultrasound pre-treatment in reducing bitterness levels and alkaloid concentrations in Lupin seeds. Moreover, the process preserves the essential nutrients present in Lupin, thereby enhancing its potential as a valuable source of protein and other nutrients for human consumption and animal feed. This study contributes to the development of sustainable and efficient pre-treatment methods for Lupin, opening avenues for its broader utilization in food, feed, and various industrial applications.

References

Alothman, M., Bhat, R., & Karim, A. A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chemistry, 115(3), 785–788. https://doi.org/10.1016/j.foodchem.2008.12.005

Anonymous. (2002). Standard methods of ınternational association for cereal science and technology. ICC.

Anonymous. (2010). American Oil Chemists’ Society. AOCS.

Bertoglio, J. C., Calvo, M. A., Hancke, J. L., Burgos, R. A., Riva, A., Morazzoni, P., Ponzone, C., Magni, C., & Duranti, M. (2011). Hypoglycemic effect of lupin seed γ-conglutin in experimental animals and healthy human subjects. Fitoterapia, 82(7), 933–938. https://doi.org/10.1016/j.fitote.2011.05.007

Bhargava, N., Mor, R. S., Kumar, K., & Sharanagat, V. S. (2021). Advances in application of ultrasound in food processing: A review. Ultrasonics Sonochemistry, 70(June 2020), 105293. https://doi.org/10.1016/j.ultsonch.2020.105293

Carvajal-Larenas, F. E., Nout, M. J. R., van Boekel, M. A. J. S., Koziol, M., & Linnemann, A. R. (2013). Modelling of the aqueous debittering process of Lupinus mutabilis Sweet. Lwt, 53(2), 507–516. https://doi.org/10.1016/j.lwt.2013.03.017

Córdova-Ramos, J. S., Glorio-Paulet, P., Hidalgo, A., & Camarena, F. (2020). Effect of technological process on antioxidant capacity and total phenolic content of Andean lupine (Lupinus mutabilis Sweet). Scientia Agropecuaria, 11(2), 157–165. https://doi.org/10.17268/SCI.AGROPECU.2020.02.02

Dorman, H. J. D., Peltoketo, A., Hiltunen, R., & Tikkanen, M. J. (2003). Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chemistry, 83(2), 255–262. https://doi.org/10.1016/S0308-8146(03)00088-8

Ertaş, N., & Bilgiçli, N. (2014). Effect of different debittering processes on mineral and phytic acid content of lupin (Lupinus albus L.) seeds. Journal of Food Science and Technology, 51(11), 3348–3354. https://doi.org/10.1007/s13197-012-0837-2

Estivi, L., Brandolini, A., Condezo-Hoyos, L., & Hidalgo, A. (2022). Impact of low-frequency ultrasound technology on physical, chemical and technological properties of cereals and pseudocereals. Ultrasonics Sonochemistry, 86(November 2021), 106044. https://doi.org/10.1016/j.ultsonch.2022.106044

Ferchichi, N., Toukabri, W., Vrhovsek, U., Nouairi, I., Angeli, A., Masuero, D., Mhamdi, R., & Trabelsi, D. (2021). Proximate composition, lipid and phenolic profiles, and antioxidant activity of different ecotypes of Lupinus albus, Lupinus luteus and lupinus angustifolius. Journal of Food Measurement and Characterization, 15(2), 1241–1257. https://doi.org/10.1007/s11694-020-00722-8

Goula, A. M., Ververi, M., Adamopoulou, A., & Kaderides, K. (2017). Green ultrasound-assisted extraction of carotenoids from pomegranate wastes using vegetable oils. Ultrasonics Sonochemistry, 34, 821–830. https://doi.org/10.1016/j.ultsonch.2016.07.022

INEN-2-390. (2005). Grano desamargado de chocho Norma Tecnica Ecuatoriana Leguminosas Grano desamargado de chocho. Instituto Ecuatoriano de Normalizacion.

Kamberoğlu, N., Olceşer, C. B., Düzyol, F., Cücen, Z., Hajıyev, V., & Uguzdogan, E. (2024). Extraction of pectin from rosehip (Rosa canina L.) fruit and waste. Pamukkale University Journal of Engineering Sciences, 30(1), 109–118. https://doi.org/10.5505/pajes.2023.93027

Karacaoğlu, C., Gürsoy, O., & Yılmaz, Y. (2016). Ultrasonikasyon Destekli Vakum İ mpregnasyon ( Emdirme ) Tekniği ile Muamele İşleminin Kivi Dilimlerinin Kuruma Kinetiği Üzerine Etkisi. 14(3), 256–266.

Karara, H. A. (1987). An Efficient Method for the Extraction of Alkaloids from Bitter Lupin Seed. European Journal of Lipid Science and Technology, 8(11), 442–446. https://doi.org/https://doi.org/10.1002/lipi.19870891107

Lampart-Szczapa, E., Korczak, J., Nogala-Kalucka, M., & Zawirska-Wojtasiak, R. (2003). Antioxidant properties of lupin seed products. Food Chemistry, 83(2), 279–285. https://doi.org/10.1016/S0308-8146(03)00091-8

Lowry, O. H., Rosenbrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193(1), 265–275. https://doi.org/10.1016/s0021-9258(19)52451-6

M. van de Noort. (2016). Lupin: An Important Protein and Nutrient Source. In Sustainable Protein Sources (pp. 165–183). Academic Press. https://doi.org/https://doi.org/10.1016/C2014-0-03542-3

Miano, A. C., Rojas, M. L., & Augusto, P. E. D. (2019). Using ultrasound for improving hydration and debittering of Andean lupin grains. Journal of Food Process Engineering, 42(6), 1–5. https://doi.org/10.1111/jfpe.13170

Navarro del Hierro, J., Herrera, T., García-Risco, M. R., Fornari, T., Reglero, G., & Martin, D. (2018). Ultrasound-assisted extraction and bioaccessibility of saponins from edible seeds: quinoa, lentil, fenugreek, soybean and lupin. Food Research International, 109(January), 440–447. https://doi.org/10.1016/j.foodres.2018.04.058

Osorio, C. E., & Till, B. J. (2022). A Bitter-Sweet Story: Unraveling the Genes Involved in Quinolizidine Alkaloid Synthesis in Lupinus albus. Frontiers in Plant Science, 12(January), 1–8. https://doi.org/10.3389/fpls.2021.795091

Siger, A., Czubinski, J., Kachlicki, P., Dwiecki, K., Lampart-Szczapa, E., & Nogala-Kalucka, M. (2012). Antioxidant activity and phenolic content in three lupin species. Journal of Food Composition and Analysis, 25(2), 190–197. https://doi.org/10.1016/j.jfca.2011.10.002

Topdaş, E. F., & Şengül, M. (2019). Katı-Sıvı Ekstraksiyonunda Kullanılan Modern Teknikler ve Bu Teknikler Arasında Ultrason Yardımlı Ekstraksiyonun Yeri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 50(2), 201–216. https://doi.org/10.17097/ataunizfd.466649

Villacrés, E., Quelal, M. B., Fernández, E., Garcìa, G., Cueva, G., & Rosell, C. M. (2020). Impact of debittering and fermentation processes on the antinutritional and antioxidant compounds in Lupinus mutabilis sweet. LWT, 131(June), 109745. https://doi.org/10.1016/j.lwt.2020.109745

Wang, S., & Clements, J. (2008). Antıoxidant Activities of Lupin Seeds. In J. A. Palta & J. D. Berger (Eds.), 12th International Lupin Conference (pp. 14–18). International Lupin Association.

Yarpuz, D. (2011). Researches On Gluten Free Bread Productıon. Selcuk University.

Yaver, E., & Bilgiçli, N. (2021). Ultrasound-treated lupin (Lupinus albus L.) flour: Protein- and fiber-rich ingredient to improve physical and textural quality of bread with a reduced glycemic index. Lwt, 148(May). https://doi.org/10.1016/j.lwt.2021.111767

Yorgancılar, M., Atalay, E., & Babaoğlu, M. (2009). Mineral content of debittered white lupin (Lupinus albus L.) seeds. Selcuk Journal of Agriculture and Food Sciences, 23(50), 10–15. http://repo.iain-tulungagung.ac.id/5510/5/BAB 2.pdf

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Published

15.10.2024

How to Cite

Baltacıoğlu, C., & Özcan Tarım, A. (2024). Debittering Process of Lupin (Lupinus albus l.) by Ultrasound Pre-treatment. Turkish Journal of Agriculture - Food Science and Technology, 12(10), 1673–1678. https://doi.org/10.24925/turjaf.v12i10.1673-1678.6921

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Research Paper