Effects of Different Treatments on Germination and Seedling Development of Moringa (Moringa oleifera L.) Seeds
DOI:
https://doi.org/10.24925/turjaf.v12is1.2076-2081.7052Keywords:
Moringa oleifera, Germination, GA3, PEG, Microbial fertilizerAbstract
This study consists of two phases: moringa seed germination and seedling development. Firstly, four different treatments (a: Soaking in warm water at 35 ℃ for 24 hours; b: 24 hours in 1000 ppm GA3 solution; c: -1MPa polyethylene glycol (PEG) solution for 24 hours; d: 2500 ppm microbial fertilizer solution for 24 hours) were applied to the seeds in addition to the control treatment. In germination trials carried out under controlled conditions, the temperature was set at 25 ℃ and the relative humidity was above 80%. After seed sowing, germination rate, time and germination energy were determined for each treatment. After germination, the seedlings were transferred to growing medium containing peat and perlite in a 1:1 ratio. Then, NPK, NPK + 2500 ppm microbial fertilizer and NPK + 5000 ppm microbial fertilizer were applied to the seedlings six times with 15 days intervals. After 90 days, plant height, stem diameter, chlorophyll content, stem, root wet and dry weights were determined. The results of the study showed that the best results for seed germination rate, time and energy were obtained by soaking the seeds in warm water at 35 ℃ for 24 hours before sowing in vials, followed by soaking in 1000 ppm GA3 solution for 24 hours. Considering the parameters examined in terms of seedling growth and development, the use of standard NPK together with microbial fertilizer gave better results compared to standard NPK. As a result of this study, it was recommended to keep the seeds in warm water at 35℃ for 24 hours for seed germination and to use standard NPK with microbial fertilizer for seedling growth.
References
Afzal, M. I., & Iqbal, M. A. (2015). Plant nutrients supplementation with foliar application of allelopathic water extracts improves wheat (Triticum aestivum L.) yield. Advance in Agriculture and Biolog, 4, 64–70. https://doi.org/10.15192/PSCP.AAB.2015.4.2.6470
Ahmad, T. A., Ahmad, F. K., Rasul, K. S., Aziz, R. R., Omer, D. A., Tahir, N. A. R., & Mohammed, A. A. (2020). Effect of some plant extracts and media culture on seed germination and seedling growth of Moringa oleifera. Journal of Plant Production, 11(7), 669-674. https://doi.org/10.21608/jpp.2020.110586
Alshoaibi, A. (2021). Seed germination, seedling growth and photosynthetic responses to temperature in the tropical tree Moringa oleifera and Its relative desert, Moringa peregrina. Egyptian Journal of Botany, 61(2), 541-551. https://doi.org/10.21608/ejbo.2021.63271.1631
Altunlu, H. (2021). Mikrobiyal gübre ve vermikompost uygulamalarının baş salata (Lactuca sativa L. var capitata) yetiştiriciliğinde bitki gelişimi, verim ve nitrat içeriğine etkisi. Mediterranean Agricultural Sciences, 34(1), 135-140. https://doi.org/10.29136/mediterranean.801439
Barminas, J. T., Milam Charles, & Emmanuel D. (1998). Mineral composition of non-conventional leafy vegetables. Plant Foods for Human Nutrition 53 (1): 29-36. https://doi.org/10.1023/A:1008084007189
Barraza, A. (2017). Germinación de semillas de moringa (Moringa oleifera Lam.) en diferentes tiempos de imbibición en agua. Revista UDCA Actualidad & Divulgación Científica, 20(1), 71-77. https://doi.org/10.31910/rudca.v20.n1.2017.64
Basra, S. M. A., Iftikhar, M. N., & Irfan, A. (2011). Potential of moringa (Moringa oleifera) leaf extract as priming agent for hybrid maize seeds. International Journal of Agriculture and Biology 13(6), 1006-1010.
Booth F. E., & Wickens G. E. (1988). Non-timber uses of selected arid zone trees and shrubs in Africa (No. 19). Food and Agriculture Organization of the United Nations.
Culver, M., Fanuel, T. & Chiteka, A. Z. (2012). Effect of moringa extract on growth and yield of tomato. Greener Journal of Agricultural Sciences, 2(5), 207–211.
Ellis, R. H., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9(2), 373-409.
Fahey, J. W. (2005). Moringa oleifera: A Review of the medicinal evidence for its nutritional, therapeutic and prophylactic properties, Trees for life Journal, 1 (5), 1-15.
Foidl, N., Makkar, H. P. S., & Becker, K. (2001). The potential of Moringa oleifera for agricultural and industrial uses. In: Fuglie, L.J. (eds.), The miracle tree: the multiple attributes of moringa, pp: 45–76. Wageningen, The Netherlands.
Fuglie L. J. (1999). The miracle tree: Moringa oleifera: Natural nutrition for the tropics. Church World Service.
Gunes, E., Gubbuk, H., Ayala-Silva, T., Gozlekci, S., & Ercisli, S. (2013). Effects of various treatments on seed germination and growth of carob (Ceratonia siliqua L.). Pakistan Journal of Botany, 45(4), 1173-1177.
Hassanein, A. M. A., & Al-Soqeer, A. A., 2017. Evaluation of seed germination and growth characteristics of Moringa oleifera and M. peregrina under laboratory, greenhouse and field conditions. International Journal of Agriculture and Biology, 19(4), 873–879. https://doi.org/10.17957/IJAB/15.0381
Javorekova, S., Makova, J, Medo, J., Kovacsova, S., Charousova, I., & Horak, J. (2015). Effect of bio-fertilizers application on microbial diversity and physiological profiling of microorganisms in arable soil. Eurasian Journal of Soil Science 4, 54-61. https://doi.org/10.18393/ejss.07093
Karaguzel, O., Baktir, I., Cakmakci, S., Ortacesme, V., Aydınoglu, B., &Atik, M. (2002). Effects of scarification methods, temperature and sowing date on some germination characteristics of Lupinus varius L. In:2nd National Congress on Ornamental Plants, October 22-24, Antalya, Turkey. pp: 40-47.
Katoriya, R. S., Singh, K. V., & Singh, R. P. (2021). Effect of seed priming on drumstick (Moringa oleifera L.) seedling growth with different concentrations of gibberellic acid. The Pharma Innovation, 10(11), 1817-1821.
Korsor, M., Ntahonshikira, C., Kwaambwa, H. M., & Bello, H. M. (2016). Comparative performance of Moringa oleifera and Moringa ovalifolia seeds and seedlings establishment in central Namibia. Net Journal of Agricultural Science, 4(2), 35–44.
Montilla-Mota, J. J., Amundaray, W. G., & Estiven, G. C. (2017). Tratamientos pregerminativos en semillas de moringa y su efecto en variables agronómicas. Pastos Forrajes 40 (3), 188–194.
Mubvuma, M. T., Mapanda, S., & Mashonjowa, E. (2013). Effect of storage temperature and duration on germination of moringa seeds (Moringa oleifera). Greener Journal of Agricultural Sciences, 3(5), 427–432. https://doi.org/10.15580/GJAS.2013.3.121912328
Onyekwelu, J. C., & Olabiwonnu, A. A. (2010). Seed germination and early growth of Moringa oleifera seedlings. Forests and Forest Products Journal, 3, 12–19.
Núñez-Gastélum, J. A., Arguijo-Sustaita, A. A., López-Díaz, J. A., Díaz-Sánchez, Á. G., Hernández-Peña, C. C., & Cota-Ruiz, K. (2023). Seed germination and sprouts production of Moringa oleifera: A potential functional food?. Journal of the Saudi Society of Agricultural Sciences, 22(4), 223-230. https://doi.org/10.1016/j.jssas.2022.12.002
Singh, S., Mishra, S. P., Singh, P. & Prasad, R. S. (2013). Moringa oleifera leaf extract as biostimulant for increasing pea yield. Indian Forester 139, 562–563.
Sreehari, S., Singh, D., Wesley, C. J., & Bahadur, V. (2022). Effect of seed germination and seedling vigour in different soil media for the establishment of annual drumstick (Moringa oleifera L.) cv. PKM-1. International Journal of Environment and Climate Change, 12, 1401-1407. https://doi.org/10.9734/ijecc/2022/v12i1131119
Sreeramulu, N., Godwin, D., & Mtotomwema, K. (1983). Effect of cooking on the nutritive value of common food plants of Tanzania: Part 1-Vitamin C in some of the wild green leafy vegetables. Food Chemistry, 10(3): 205-210. https://doi.org/10.1016/0308-8146(83)90056-0
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.