Identification of InDel Variants of CSN1S1 and CMTM2 Genes Associated with Prolificacy in Hair, Honamlı, and Kabakulak Goats
DOI:
https://doi.org/10.24925/turjaf.v12i9.1577-1582.7087Keywords:
CMTM2, Multiple births, Casein, Goat, Molecular genotyping, PCRAbstract
Variations such as insertion and deletion (InDel) in gene regions of alpha S1 casein (CSN1S1) and CKLF like MARVEL transmembrane domain protein 2 (CMTM2) may affect litter size in goats. n this study, a total of 210 animals belonging to Hair (KIL, 66 samples), Honamlı (HNM, 74 samles), and Kabakulak (KBK, 70 samples) goats were genotyped by Polymerase Chain Reaction (PCR) method to detect InDel variations of CSN1S1 and CMTM2 genes. All goat populations showed polymorphism in terms of both genes in the current study in which the frequency of the desired genotype (II) for the CSN1S1 ranged from 0.10 (KBK) to 0.12 (KIL and HNM). The lowest and highest observed heterozygosity (HO) values were observed in KBK (0.53) and KIL (0.65) goats, respectively. II genotype frequency varied between 0.09 (KIL) and 0.29 (KBK) in terms of the CMTM2 gene while H0 values ranged from 0.41 (KIL) to 0.58 (KBK). All goat populations turned out to be under Hardy-Weinberg equilibrium regarding both genes. The results of the current study showed that KIL, HNM, and KBK goats conserve a sufficient genetic variation together with variable frequencies of the desired II genotype in terms of CSN1S1 and CMTM2 genes. Therefore, it is also thought that variations of the CSN1S1 and CMTM2 genes may be utilized in Marker Assisted Selection (MAS) studies to improve litter size in studied native goat breeds.
References
Ağyar, O., & Kırıkçı, K. (2022). Investigation of FecXI mutation by PCR-RFLP method in Awassi sheep breed. Bahri Dağdaş Hayvancılık Araştırma Dergisi, 11, 88-93.
Aslan, M., Demir, E., & Karslı, T. (2022). Microsatellite diversity and restriction enzyme-based polymorphisms of MHC loci in some native Turkish goats. Journal of Agricultural Sciences, 28, 626-634. https://doi.org/10.15832/ankutbd.924222
Atay, S., Yurdagül, K. G., Bilginer, Ü., Karslı, T., & Demir, E. (2023). InDel variations of PRL and GHR genes associated with litter size in Pirlak sheep breed. Tekirdağ Ziraat Fakültesi Dergisi, 20, 890-897. https://doi.org/10.33462/jotaf.1226643
Ayçiçek, S. T. (2023). Küçük hücreli dışı akciğer kanserlerinde immunhistokimyasal CMTM6 ve PD-L1’in klinikopatolojik ve prognostik önemi. (Tez No. 777208) [Tıpta Uzmanlık Tezi, Necmettin Erbakan Üniversitesi). Yükseköğretim Kurulu Ulusal Tez Merkezi.
Bozkaya, F., Gurler, S., & Yerturk, M. (2013). Genetic variability of CSN1S1 gene in goat populations raised in Southeastern Region of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, 147-152. https://doi.org/10.9775/kvfd.2012.7402
Cui, Y., Yan, H., Wang, K., Xu, H., Zhang, X., Zhu, H., Liu, J., Qu, L., Lan, X., & Pan, C. (2018). Insertion/deletion within the KDM6A gene is significantly associated with litter size in goat. Frontiers in Genetics, 9, 91. https://doi.org/10.3389/fgene.2018.00091
Çelikeloğlu, K., Tekerli, M., Erdoğan, M., Koçak, S., Hacan, Ö., & Bozkurt, Z. (2021). An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep. Archives Animal Breeding, 64, 223-230. https://doi.org/10.5194/aab-64-223-2021
Demir, E. (2024). Microsatellite-based bottleneck analysis and migration events among four native Turkish goat breeds, Archives Animal Breeding, 67, 353–360. https://doi.org/10.5194/aab-67-353-2024
Demir, E., Karslı, B. A., Karslı, T., & Balcioğlu, M. S. (2020). Determination of SacII and MboII polymorphisms in the Nerve Growth Factor (NGF) gene in four native Turkish goat populations. Mediterranean Agricultural Sciences, 33, 145-148. https://doi.org/10.29136/mediterranean.633476
Demirbaş, N., Tosun, D., & Taşkın, T. (2009). AB üyesi kimi Akdeniz ülkeleri ve Türkiye’de koyun-keçi üretim ve dış ticareti. Hayvansal Üretim, 50, 45-53.
Erduran, H., & Kırbaş, M. (2010). Hair goat breeding and improvement studies in Konya province. National Goat Congress, Çanakkale, 193-197.
Gedik, Y. (2023). Investigation for mutation in BMPR-1B (FecB) fecundity gene in Awassi sheep. Selcuk Journal of Agriculture and Food Sciences, 37, 242-247.
Günlü, A., & Alaşahan, S. (2010). Türkiye’de keçi yetiştiriciliği ve geleceği üzerine bazı değerlendirmeler. Veteriner Hekimler Dergisi, 81, 15-20.
He, L., Kang, Z., Kang, Y., Xiang, W., Pan, C., Chen, H., Zhu, H., Qu, L., Lan, X., & Song, X. (2020). Goat CMTM2: mRNA expression profiles of different alternative spliced variants and associations analyses with growth traits. 3 Biotech, 10, 1-10. https://doi.org/10.1007/s13205-020-2125-6
Kang, Z., Zhang, S., He, L., Zhu, H., Wang, Z., Yan, H., & Pan, C. (2019). A 14-bp functional deletion within the CMTM2 gene is significantly associated with litter size in goat. Theriogenology, 139, 49-57. https://doi.org/10.1016/j.theriogenology.2019.07.026
Karadağ, O. (2016). Honamlı keçisinin bazı morfolojik özellikleri döl verimi ve kazein genleri polimorfizmi bakımından incelenmesi. (Tez No. 420333) [Doktora Tezi, Namık Kemal Üniversitesi]. Yükseköğretim Kurulu Ulusal Tez Merkezi.
Karslı, T., & Balcıoğlu, M. S. (2010). An investigation of presence of FecB allele on BMPR-IB (Booroola) gene raised in Turkey in six local sheep breeds using PCR-RFLP method. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, 1033-1036.
Karslı, T., Şahin, E., Karslı, B. A., Eren, M. G., & Balcıoğlu, M. S. (2011). Kangal ve Güney Karaman koyunlarında FecB, FecXG, FecXH allellerinin PZR-RFLP yöntemi kullanılarak araştırılması. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 51, 71-80.
Karslı, T., Şahin, E., Karslı, B. A., Alkan, S., & Balcıoğlu, M. S. (2012). An investigation of mutations (FecXG, FecXI, FecXH, FecXB) on BMP-15 gene in some local sheep breeds raised in Turkey. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 25(1), 29-33.
Karslı, T., Demir, E., Fidan, H. G., Aslan, M., Karslı, B. A., Arik, I. Z., Sahin Semerci, E., Karabag, K., & Balcioglu, M. S. (2020). Determination of genetic variability, population structure and genetic differentiation of indigenous Turkish goat breeds based on SSR loci. Small Ruminant Research, 190, 106147. https://doi.org/10.1016/j.smallrumres.2020.106147
Kirikçi, K. (2023). Investigation of BMP15 and GDF9 gene polymorphisms and their effects on litter size in Anatolian sheep breed Akkaraman. Turkish Journal of Veterinary & Animal Sciences, 47, 248-254. https://doi.org/10.55730/1300-0128.4292
Li, M., Luo, F., Tian, X., Yin, S., Zhou, L., & Zheng, S. (2020). Chemokine-like factor-like MARVEL transmembrane domain-containing family in hepatocellular carcinoma: latest advances. Frontiers in Oncology, 10, 595973. https://doi.org/10.3389/fonc.2020.595973
Miller, S., Dykes, D., & Plesky, H. A. (1988). Simple salting out procedure for extracting DNA from human cells. Nucleic Acids Research, 16, 1215-1215.
Rahmatalla, S. A., Arends, D., & Brockmann, G. A. (2022). Genetic and protein variants of milk caseins in goats. Frontiers in Genetics, 13, 995349. https://doi.org/10.3389/fgene.2022.995349
TÜIK. (2023). Türkiye İstatistik Kurumu, İstatistik Tablolar, Hayvansal Üretim İstatistikleri (Yıllık), Küçükbaş Hayvan Sayıları. https://data.tuik.gov.tr/Kategori/GetKategori?p=tarim-111&dil=1 (Erişim Tarihi: 06.05.2024).
Wang, K., Yan, H., Xu, H., Yang, Q., Zhang, S., Pan, C., Chen, H., Zhu, H., Liu, J., Qu, L., & Lan, X. (2018). A novel indel within goat casein alpha S1 gene is significantly associated with litter size. Gene, 671, 161-169. https://doi.org/10.1016/j.gene.2018.05.119
Yeh, F. C., Yang, R. C., Boyle, T. B. J., Ye, Z. H., & Mao, J. X. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
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