Investigation of the Bioactive Compounds of Wild Grown Prickly Pear (Opuntia ficus-barbarica A.Berger) Fruits and Peels in the İskenderun Region
DOI:
https://doi.org/10.24925/turjaf.v12is4.2764-2769.7183Keywords:
Antioxidant, Phenolic, Flavonoid, Prickly Pear, Opuntia Ficus-Indica L.Abstract
Due to global warming, there is increasing interest in plant-based foods with low water requirements and valuable bioactive compounds. This study evaluated the total phenolic content, flavonoid content, and antioxidant activities of fruit (M) and peel (K) extracts from prickly pear (Opuntia ficus-barbarica A.Berger). Initially, samples were extracted using methanol. The phenolic contents in methanol extracts were determined as 835.66±21.12 mg GAE/100 g in K and 760.66±17.61 mg GAE/100 g in M, while total flavonoid contents were measured as 222.00±4.35 µg QE/g in K and 204.76±0.87 µg QE/g in M. Antioxidant activity was assessed using two methods: free radical scavenging activity (DPPH) and Cupric ion reducing antioxidant capacity (CUPRAC), with reduction capacities calculated as 40.93±1.46 µg TE/mL extract for M and 80.11±2.03 µg TE/mL extract for K. The IC50 values of fruit and peel extracts were calculated and compared with standard BHT (Butylated hydroxytoluene), with the peel extract showing the closest value. Results demonstrated that both fruit and peel of Opuntia ficus-barbarica contain significant amounts of bioactive compounds and exhibit potent antioxidant activity. This study reveals the potential of this traditionally used medicinal plant as a health-promoting food component and natural antioxidant source.
References
Al Juhaimi, F., Ghafoor, K., Uslu, N., Ahmed, IAM., Babiker, EE., Özcan, MM., & Fadimu, GJ. (2020). The effect of harvest times on bioactive properties and fatty acid compositions of prickly pear (Opuntia ficus-barbarica A. Berger) fruits. Food chemistry, 303, 125387. https://doi.org/10.1016/j.foodchem.2019.125387
Belviranlı, B. (2016). Hint İnciri (Opuntia ficus-indica L.) Meyvesi ve Tohumlarının Bazı Fiziko-Kimyasal Özellikleri Üzerine Lokasyonun Etkisi. Doktora tezi, Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Gıda Mühendisliği Anabilim Dalı.
Blois, MS. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200.
Capar, TD., Dedebas, T., Kavuncuoglu, H., Karatas, SM., Ekici, L. & Yalcin, H. (2023). Phenolic Components, Mineral Composition, Physicochemical, and Bioactive Properties of Opuntia ficus-indica with Different Drying Methods. Erwerbs-Obstbau, 65(2), 347-353.
Dengiz, T., & Zengin, H. (2016). Hint inciri (Opuntia ficus-indica) Meyve Suyunun Kimyasal ve Antioksidan Özelliklerinin İncelenmesi. İstanbul Aydın Üniversitesi Dergisi 30, 125-150.
Duggan, MR., Lee-Soety, JY., Anderson, MJ. (2017). Personality types in Budgerigars, Melopsittacus undulatus. Behav Processes. 138, 34-40. https://doi.org/10.1016/j.beproc.2017.02.003
Dumanoğlu, Z., Güzel, Ü., & Çakır, A. (2020). Doğu Akdeniz Bölgesinde Yetişen Dikenli incir (Opuntia ficus-indica L.) Tohumlarının Bazı Fiziksel Özelliklerinin Belirlenmesi Üzerine Bir Araştırma. Türk Doğa ve Fen Dergisi, 9 (Özel Sayı), 28-33. https://doi.org/10.46810/tdfd.708523
Duru, B., & Türker, N. (2005). Changes in Physical Propartice and Cheminal Composition of Cactus Pear (Opuntia ficus-indica) During Maturation. Journal of The Professiona Association for Cactus Development, 7, 22-33. https://doi.org/10.56890/jpacd.v7i.285
El-Mostafa, K., El Kharrassi, Y., Badreddine, A., Andreoletti, P., Vamecq, J., El Kebbaj, MHS., ... & Cherkaoui-Malki, M. (2014). Nopal cactus (Opuntia ficus-indica) as a source of bioactive compounds for nutrition, health and disease. Molecules, 19(9), 14879-14901. https://doi.org/10.3390/molecules190914879
Emami Bistgani, Z., Siadat, A., Bakhshandeh, M., Ghasemi Pirbaloutu, A., Hashemi, M. (2017). Morpho-physiological and phytochemical traits of (Thymus daenensis Celak.) in response to deficit irrigation and chitosan application. Acta Physiol. Plant., 39, 1-13. https://doi.org/10.1007/s11738-017-2526-2
Ergün, F. (2022). Satureja hortensis L.’nin Fitokimyasallarının ve Antioksidan Kapasitesinin Belirlenmesi. Turkish Journal of Agriculture-Food Science and Technology, 10, 2635-2639. https://doi.org/10.24925/turjaf.v10isp1.2635-2639.5577
Ergün, F. (2021). Cotoneaster transcaucasicus pojark. determination of bioactive component amounts and antioxidant activities in fruit extracts. Turkish Journal of Agriculture - Food Science and Technology, 9 (7), 1258-1263. https://doi.org/10.24925/turjaf.v9i7.1258-1263.4432
Fernández-López, JA., Almela, L., Obón, JM., & Castellar, R. (2010). Determination of antioxidant constituents in cactus pear fruits. Plant Foods for Human Nutrition, 65, 253-259. https://doi.org/10.1007/s11130-010-0189-x
Franco-Vásquez, DC., Bosquez-Molina, E., & Ventura-Aguilar, RI. (2020). Standardization of Phenols and Flavonoids Extraction from Opuntia ficus-indica Mill cv. Atlixco and Identification by Mass Spectrometry. Journal of the Mexican Chemical Society, 64 (4), 272-282. https://doi.org/10.29356/jmcs.v64i4.1203
Galati, EM., Mondello, MR., Giuffrida, D., Dugo, G., Miceli, N., Pergolizzi, S., & Taviano, MF. (2003). Chemical characterization and biological effects of Sicilian Opuntia ficus indica (L.) Mill. fruit juice: antioxidant and antiulcerogenic activity. Journal of agricultural and food chemistry, 51(17), 4903-4908. https://doi.org/10.1021/jf030123d
Genç S. and Soysal MI. (2018). Parametric and nonparametric post hoc tests. BSJ Eng Sci. 1(1), 18-27.
Gouws, CA., D'cunha, NM., Georgousopoulou, E., Thomas, J., Mellor, D., Roach, PD., & Naumovskı, N. (2017). The effect of drying methods on bioactive properties in three varieties of prickly pear (Oppuntia ficus indica). Journal of Nutrition and Intermediary Metabolism, 8, 107.
Guardia, T., Rotelli, AE., Juarez, AO., & Pelzer, LE. (2001). Anti-inflammatory properties of plant flavonoids. Effects of rutin, quercetin and hesperidin on adjuvant arthritis in rat. Il farmaco, 56(9), 683-687. https://doi.org/10.1016/s0014-827x(01)01111-9
Güven, C. (2017). Doğu Akdeniz Bölgesi'nden Selekte Edilen Bazı Dikenli İncir (Opuntia ficus indica (L.) Mill.) Genotiplerine Ait Meyve Sularının Kimyasal Özelliklerinin İncelenmesi (Tez no 466574). Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı. Yükseköğretim Kurulu Ulusal Tez Merkezi
Güzel, M., & Akpınar, Ö. (2017). Turunçgil kabuklarının biyoaktif bileşenleri ve antioksidan aktivitelerinin belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 7(2), 153-167. http://dx.doi.org/10.17714/gufbed.2017.07.010
Güzel, Ü. (2019). Mersin ve Çevresinde Yetişmekte Olan Bazı Dikenli İncirlerde (Opuntia ficus-indica L.) En Uygun Hasat Dönemlerinin Saptanması (Tez no 553939). Yüksek Lisans Tezi, Bingöl Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı. Yükseköğretim Kurulu Ulusal Tez Merkezi
Hernández-Urbiola, MI., Pérez-Torrero, E., & Rodríguez-García, ME. (2011). Chemical analysis of nutritional content of prickly pads (Opuntia ficus indica) at varied ages in an organic harvest. International journal of environmental research and public health, 8 (5), 1287-1295. https://doi.org/10.3390/ijerph8051287
Jana, S. (2012). Nutraceutical and Functional Properties of Cactus Pear (Opuntia spp.) and its Utilization for Food Applications. Journal of Research and Studies, 3 (2), 60-66
Jimenez-Aguilar, MD., Mujica-Paz, H., & WeltiChanes, J. (2014). Phytochemical Characterization of Prickly Pear (Opuntia spp.) and of its Nutritional and Functional Properties: A Review. Current Nutrition & Food Seience, 10 (1), 57-69. https://doi.org/10.2174/157340131001140328120952
Karababa, E., Coşkuner, Y., & Aksay, S. (2004). Some Physical Fruit Properties of Cactus Pear (Opuntia spp.) That Grow Wild in the Earstern Mediterranean Region of Turkey. Journal of the Professional Association for Cactus Development, 6, 1-8. https://doi.org/10.56890/jpacd.v6i.293.
Martín-Lara, MA., Ortuñob, N., Conesa. JA. (2018). Volatile and semivolatile emissions from the pyrolysis of almond shell loaded with heavy metals. Sci. Total Environ., 1, 613-614:418-427. https://doi.org/10.1016/j.scitotenv.2017.09.116
Moreno, MIN., Isla, MI., Sampietro, AR., & Vattuone, MA. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71(1-2), 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
Okur, ME., Karakaş, N., Karadağ, AE., Uludağ, D., & Polat, DÇ. (2019). Investigation of antioxidant and cytotoxic activities of Opuntia ficus-indica (L.) Mill. fruit extract. Istanbul Journal of Pharmacy, 49 (3), 154-160. https://doi.org/10.26650/IstanbulJPharm.2019.19035
Park, C., Kwak, B., Park, S., Kim, H., & Rhyu, D. (2013). Comparison of Biological Activites of Opuntia humifusa and Opuntia ficus-indica. Korean Journal of Plant Resources 26(5), 519-525. https://doi.org/10.7732/kjpr.2013.26.5.519
Pietrzak, W., Nowak, R., Gawlik-Dziki, U., Lemieszek, M. & Rzeski, W. (2017). LC-ESI-MS/MS Identification of Biologically Active Phenolic Compounds in Mistletoe Berry Extracts from Different Host Trees. Molecules 22 (4), 624. https://doi.org/10.3390/ molecules22040624
Sharma, A., Shahzad, B., Kumar, V., Kohli, SK., Sidhu, GPS., Bali, AS., Handa, N., Kapoor, D., Bhardwaj, R. & Zheng, B. (2019). Phytohormones regulate accumulation of osmolytes under abiotic stress. Biomolecules, 9: 285. https://doi.org/10.3390/biom9070285.
Slinkard, K. & Singleton, VL. (1977). Total phenol analyses: automation and comparison with manual methods. American journal of enology and viticulture, 28, 49-55. https://doi.org/10.5344/ajev.1977.28.1.49
Tilahun, Y. & Walegerima, G. (2018). Pharmacological Potential of Cactus Pear (Opuntia ficus indica) a Review. Journal of pharmacognosy and phytochemistry, 7(3), 1360-1363
Toplu, C., Serce, S., Ercisli, S., Kamiloglu, O. & Sengul, M. (2009). Phenotypic variation in physico-chemical properties among cactus pear fruits (Opuntia ficus-indica (L.) Miller) from Turkey. Pharmacognosy Magazine, 5 (20s), 400-406.
Trombetta, D., Puglia, C., Perri, D., Licata, A., Pergolizzi, S., Lauriano, ER., ... & Bonina, FP. (2006). Effect of polysaccharides from Opuntia ficus-indica (L.) cladodes on the healing of dermal wounds in the rat. Phytomedicine, 13 (5), 352-358. https://doi.org/10.1016/j.phymed.2005.06.006
Tütüncü, M., Sarıer, A., İmrak, B., Çömlekçioğlu, S., Küden, A. & Küden. AB. (2016). Adana Bölgesinden Selekte Edilen Dikenli İncirlerin Meyve Özelliklerinin Belirlenmesi. Anadolu Tarım Bilimleri Dergisi, 31(2), 183-190. https://doi.org/10.7161/omuanajas.260973
Uzun, H., & Şengül, S. (1994). Frenk İnciri Yetiştiriciliği. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 7, 73-89.
Ünalan, Ö. (2023). Tropikal Meyve Yetiştiriciliğinin Türk Halk Hekimliği Üzerindeki Etkileri. Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, 13 (1), 75-85. https://doi.org/10.31020/mutftd.1206563
Vasquez, DCF. (2017). Estandarización del proceso de extracción e identificación de Flavonoides en Nopal (Opuntia ficus-indica Mill.) Cultivar atlixco desespinado. (Tesis de Maestría, Universidad Autónoma Metropolitana), https://doi.org/10.24275/uami.hm50tr74r
Wang, H., Zhou, X., Liu, Y., Xie, W., Yang, D., Huo, D., ... & Wang, R. (2024). Identification and molecular docking of xanthine oxidase and α‐glucosidase inhibitors in Opuntia ficus‐indica fruit. Journal of Food Science, 89(7), 4192-4204. https://doi.org/10.1111/1750-3841.17144
Yaribeigi Darvishvand, S. (2020). The use of Opuntia ficus-indica mucilage edible coating to extend the shelf-life of packaged strawberry and red raspberry (Master's thesis, Universitat Politècnica de Catalunya).
Yeddes, N., Chérif, JK., Guyot, S., Sotin, H. & Ayadi, MT. (2013). Comparative study of antioxidant power, polyphenols, flavonoids and betacyanins of the peel and pulp of three Tunisian Opuntia forms. Antioxidants, 2(2), 37-51. https://doi.org/10.3390/antiox2020037
Yılmaz, C. (2010). Dikenli İncir (Opuntia ficus-indica L.) Yetiştiriciliği. Tarım Türk, 24, 14-16.
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