Morphological Characterization and Multivariate Analysis of Rain Fed Rice (Oryza sativa L) Genotypes in Northwest Ethiopia

Authors

DOI:

https://doi.org/10.24925/turjaf.v13i2.423-438.7274

Keywords:

Cluster, polymorphic, principal component, Shannon Weaver diversity index

Abstract

Over the years, more than 3336 rice genotypes were introduced into Ethiopia from different countries and evaluated for diseases reaction and agronomic traits. However, morphological characteristics and relationships were not well investigated among genotypes. Hence, the objective of this study was to characterize 100 rice genotypes morphologically for better identification and to classify genotypes relationships. The experiment was done using triple lattice design with 10×10 in 2021/22 main cropping season. Twenty-five qualitative morphological traits were used to characterize and 13 quantitative traits were used to categorize relationships of the studied rice genotypes. The Shannon Weaver diversity index of qualitative traits were varied from 0 to 1. Moreover, based on quantitative traits the studied rain fed rice genotypes were grouped into four distinct clusters and four principal components with Eigen value >1 were contributed 78.99% of the total variation. We recommend crossing cluster I with IV genotypes to get early-maturing besides moderate grain yield variety for short rainfall season areas. And cross cluster III with IV genotypes to get medium matured, high filled spikelet per panicle, augmented harvest index, biomass and grain yield variety for long rainfall season areas.

Author Biography

Assaye, Ethiopian Institute of Agricultural Research, Fogera National Rice Research and Training Center, Rice breeding, P.O Box: 1937, Bahir Dar, Ethiopia

Assaye Berie Mekonnen: He graduated his B.Sc. in plant science in 2014 and MSc in plant breeding in 2022 both at Bahir Dar University, Ethiopia. He was working as junior instructor from December 2, 2014 to September 22, 2016 in plant science at ministry of agriculture, Gewane ATVET College, Ethiopia. Now, he is researcher I in rice breeding in Ethiopian Institute of Agricultural Research, Fogera National Rice Research and Training Center, Woreta, Ethiopia since 2016.

References

Abebe, T. (2016). Genetic Variability and Character Association in Rain Fed Lowland Rrice (Oryza sativa L.) Genotypes at Pawe and Fogera in Ethiopia, M.Sc. Thesis, Jimma University, Ethiopia. http://10.140.5.162//handle/123456789/2793

Akbar, F., Rabbani, M. A., Shinwari, Z. K., & Khan, S. J. (2011). Genetic divergence in sesame (Sesamum indicum L.) landraces based on qualitative and quantitative traits, Pak. J. Bot., 43, 2737-2744. https://www.researchgate.net/publication/279572248

Asante, M.D. (2017). Chapter 5: breeding rice for improved grain quality, World's largest Science, Technology and Medicine Open Access book publisher, In. Tech., DOI, 10.5772/66684. https://www.intechopen.com/chapters/53681

Asmelash, Y. (2014). Determinants of adoption of upland rice varieties in Fogera district, South Gondar, Ethiopia, Journal of Agricultural Extension and Rural Development, 8(12), 332-338. https://www.researchgate.net/publication/335188558

Ayenew, A., Dejene, T., & Worede, F. (2020). Genetic divergence analyses of lowland rice genotypes in North Western Ethiopia, Afr. J. Plant Sci., 14, 165-171. http://www.academicjournals.org/AJPS

Bioversity International, IRRI, & WARDA (2007). Descriptors for wild and cultivated rice (Oryza spp.). Bioversity International, Rome, Italy; International Rice Research Institute, Los Baños, Philippines; WARDA, Africa Rice Center, Cotonou, Benin. file:///D:/Yene%20Mereja/Journals/Descriptors%20for%20wild%20and%20cultivated%20rice.pdf

Birhanu, A. (2020). Genetic Variability and Association of Traits in Upland Rice (Oryza sativa L.) Genotypes in Metema district, Northwestern Ethiopia, M.Sc. thesis, Bahir Dar University, Ethiopia. http://ir.bdu.edu.et/handle/123456789/11392

Central Statistical Agency (CSA) (2018). Report on area and production of crops (Private peasant holdings, Meher season), Addis Ababa, Ethiopia.

Chahal, G.S. & Gosal, S.S. (2002). Principles and procedures of plant breeding: biotechnological and conventional approaches, Alpha Science Int'l Ltd. 399-412. https://books.google.com.et/books?id=NzSLXFeARZ4C&printsec

Dessie, A., Worede, F., Atnaf, M., Tadesse, B., & Mengistu, G. (2019). Rice genetic improvement for different ecosystems in Ethiopia, In: Tadesse, T., Atnaf, M., Alemu, D., Tadesse, T., & Shiratori, K., (Ed.). Advances in rice research and development in Ethiopia, EIAR, Addis Ababa, Ethiopia, pp. 59-68. https://www.researchgate.net/publication/381312675

Dehghani, D., Omidi, H., & Sabaghnia, N. (2008). Graphic analysis of trait relations of canola (Brassica napusL.) using biplot method, Agronomy J.100:760-764.

Dhakal, A., Pokhrel, A., Sharma, S. & Poudel A. (2020). Multivariate analysis of phenotypic diversity of rice (Oryza sativa L.) land races from Lamjung and Tanahun Districts, Nepal, International Journal of Agronomy, Article ID 8867961, pp.8. https://doi.org/10.1155/2020/8867961

FAOSTAT (2020). Food and Agriculture Organization of the United Nations (FAO). www.fao.org/faostat/en/#data

Fentie, D.B, Ali, H.M., & Abera, B.B. (2021). Genetic divergence and cluster analysis for yield and yield contributing traits in lowland rice (Oryza sativa L.) genotypes at Fogera, Northwestern Ethiopia, Int. J. Adv. Res. Biol. Sci., 8(5), 1-11. https://www.academia.edu/87928722

Gen Stat. (2015). Genstat software 8th edition.

Ghimire, K. H., Joshi, B.K., Karkee, A., & Paudel, M.N. (2018). Morphological variation in Nepalese cold tolerant rice accessions, Proceedings of the 29th national summer crop workshop, Nepal, pp. 115 – 126. https://www.researchgate.net/publication/342439361

Guo, Y., Ren, G., Zhang, K., Li, Z., Miao, Y., & Guo, H. (2021). Leaf senescence: progression, regulation, and application, Molecular Horticulture, 1: 5-30.

IRRI (2013). Standard Evaluation System (SES) for Rice, fourth edition, Philippines, pp. 55.

Jeffers, J.N.R. (1967). Two case studies in the application of principal component analysis, Journal of the Royal Statistical Society, 16, 225–236. https://doi.org/10.1186/s43897-021-00006-9

Jolliffe, I.T. (2002). Principal component analysis. Second edition, Springer-Verlag, New York, USA, pp.518. https://www.scribd.com/document/484920903/Jolliffe

Johnson, R.A & Wichrn, D.W. (1992). Applied multivariate statistical analysis. Third edition. Prentice Hall Inc., New Jersey, U.S.A, pp.642. https://pdfarchived.net/docs/applied-multivariate-statistical-analysis-3rd-edition-4896535

Kaiser, H.F. (1958). The varimax criterion for analytic rotation in factor analysis, Psychometrika. 23,187–200. https://www.psychometricsociety.org/sites/main/files/file-attachments/kaiser_citation_classic_varimax.pdf?1582948691

Kashyap, A. & Yadav, V.K. (2020). Principal component analysis and character association for yield components in rice (Oryza sativa L.) Genotypes of salt tolerance under alkaline condition, Int.J.Curr.Microbiol.App.Sci., 9(10), 481-495. https://scite.ai/reports/principal-component-analysis-and-character-0GdmWQ18?utm_campaign=badge&utm_medium=badge&utm_source=www.ijcmas.com

Loitongbam, B., Chandra, K., Bisen P., Namrata, Thakur P., & Sandhya (2017). Breeding for low temperature stress tolerance in reproductive stage of rice (Oryza sativa L.), International Journal of Bio-resource and Stress Management, 8(6), 857-862. https://www.researchgate.net/publication/326294502

Lombard, V., Baril, C., Dubreuil, P., Blouet, F. & Zhang, D. (2000). Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration, Crop Sci., 40, 417-25. DOI:10.2135/cropsci2000.4051417x

Mahalanobis, P.C. (1936). On the generalized distance in statistics, Proceedings of the National Academy of Sciences, 2, 49-55. http://bayes.acs.unt.edu:8083/BayesContent/class/Jon/MiscDocs/1936_Mahalanobis.pdf

Mahendran, R., Veerabadhiran, P., Robin, S.., & Raveendran, M. (2015). Principal component analysis of rice germplasm accessions under high temperature stress, IJASR, 5, 355-360. https://www.cabidigitallibrary.org/doi/pdf/10.5555/20153246645

Mohammadi, S.A. & Prasanna, B.M. (2003). Analysis of genetic diversity in crop plants, salient statistical tools and considerations, Crop Sci., 43(4), 1235- 1248. https://doi.org/10.2135/cropsci2003.1235

Mondal, B., Singh, S.P., & Joshi, D. C. (2014). DUS characterization ofrice (Oryza sativa L.) using morphological descriptors and quality parameters, Outlook on Agriculture 43, 131–137. https://www.researchgate.net/publication/263013583

Nascimento W.F., Silva E.F., & Veasey, E.A. (2011). Agro-morphological characterization of upland rice accessions. Sci. Agric. (Piracicaba, Braz.), 68(6), 652-660. https://www.scielo.br/j/sa/a/wzvKDTdfVRXbGk4LF3jPkCn/?format=pdf&lang=en

Nethra, N., Prasad, R. S., & Gowda, R. (2005). Varietal characterization based on seed, seedling and plant morphological traits in rice (Oryza sativa L.) genotypes, Mysore Journal of Agricultural Science, 39, 362–367.

Noirot, M., Hamon, S. & Anthony, F. (1996). The principal component scoring: a new method of constituting a core collection using quantitative data, Genetic Resources and Crop Evolution, 43, 1–6. https://link.springer.com/article/10.1007/BF00126934

O‟Toole, J.C. & Namuco, O. S. (1983). Role of panicle exertion in water stress induced sterility, Crop Science, 2, 1093-1097.

Pachauri, A.K., Sarawgi, A.K., Bhandarkar, S., & Nair, S.K. (2020). Characterization and variability analysis of rice germplasm accessions for morphological traits. Journal of Pharmacognosy and Phytochemistry, 9(5), 1407-1413. https://www.researchgate.net/publication/344488913

Pathak, H. N. (2020). Agro-morphological diversity (qualitative traits) of Oryza sativa L. cultivated in Paiyunpata Village, Baglung, Nepal. NJST, 19(1), 9-15. File:///C:/Users/HP/Downloads/gebanath,+9-15+Hom+Nath+Pathak.pdf

Pearson, K. (1901). On lines and planes of closest fit to systems of points in space, Philos Mag A., 6, 559–572. https://pca.narod.ru/pearson1901.pdf

Rawte, S. & Saxena, R.R. (2018). Morphological Characterization of Selected Rice (Oryza sativa L.) from Core Germplasm Group of Chhattisgarh Using DUS Descriptors. Int. J. Curr. Microbiol. App. Sci., 7(10), 350-357. https://www.ijcmas.com/7-10-2018/Suman%20Rawte%20and%20Ritu%20R.%20Saxena.pdf

Rashid, K., Fhaliq, I., Farooq, M.O. & Ahsan, M.Z. (2014). Correlation and cluster analysis of some yield and yield related traits in rice (Oryza sativa L), IJASR, 3: 25-30. https://www.researchgate.net/publication/268443240

Raji, A. A. (2003). Assessment of Genetic Diversity and Heterotic Relationships in African Improved and Local Cassava (Manihot esculenta Crantz) Germplasm. A Ph.D dissertation is presented to the School of Graduate Studies of University of Ibadan, Nigeria, pp. 218.

Rebeira .S.P., Wickramasinghe H.A.M., Samarasinghe W.L.G., & Prashantha B.D.R. (2014). Diversity of grain quality Characteristics of traditional rice (Oryza sativa L.) varieties in Sri Lanka. Tropical Agricultural Research, 25(4), 570-578. https://tar.sljol.info/articles/10.4038/tar.v25i4.8062

Sahu, G.R., Burman, M., Nair, S.K., Sarawgi, A.K., & Rao, R.K. (2018). Genetic behaviour of awning character in rice (Oryza sativa L.), Int.J.Curr. Microbiol. App.Sci., 7 (5), 490- 493. file:///C:/Users/HP/Downloads/G.R%20Sahu,%20et%20al.pdf

Sarawgi & Ojha, G.C. (2016). Characterization and correlation analysis for yield and yield contributing traits in medium duration germplasm accessions of rice (Oryza sativa L.), Int. J Cur. Res., 8(06), 32382-32389. https://www.journalcra.com/sites/default/files/issue-pdf/15501.pdf

Shannon, C.E. & Weaver, W. (1949). The mathematical theory of communication, The University of Illinois, Chicago, pp. 3-24. https://raley.english.ucsb.edu/wp-content/Engl800/Shannon-Weaver.pdf

Sharma, N. & Khanna, R. (2019). Rice Grain Quality: Current Developments and Future Prospects. https://www.intechopen.com/chapters/69263

Sinha, A.K. & Mishra, P.K. (2013). Agro-morphological characterization of rice landraces variety (Oryza sativa L.) of Bankura district of West Bengal, Research in Plant Biology, 3(5): 28-36. https://www.researchgate.net/publication/275950195

Sharma, J.R. (1998). Statistical and biometrical techniques in plant breeding, New Age International limited, publishers. New Delhi, pp.432. https://books.google.com.et/books/about/Statistical_and_Biometrical_Techniques_i.html?id=9eVQAXTIRwoC&redir_esc=y

Tejaswini, K.L.Y., Manukonda, S., Ramana Rao, P. V., Kumar, S.R.R., Mohammad, L.A., & Raju S.K. (2016). Cluster analysis studies in rice (Oryza sativa L.) using wards minimum variance method, Journal of Agricultural and Crop Research. 4(9):129-139. http://www.sciencewebpublishing.net/jacr/archive/2016/December/pdf/Tejaswini%20et%20al.pdf

Tiruneh, A., Gebre-selassie W., & Tesfaye, A. (2019). Genetic diversity study on upland rice (Oryza sativa L.) genotypes based on morphological traits in Southwestern Ethiopia, Asian J. Crop Sci., 11, 17-24. https://scialert.net/fulltext/fulltextpdf.php?pdf=ansinet/ajcs/2019/17-24.pdf

Turhan, N.S. (2020). Karl Pearson’s chi-square tests, Educ. Res. Rev. 15(9), 575-580. https://files.eric.ed.gov/fulltext/EJ1267545.pdf

Worede, F., Sreewongchai, T., Phumichai, C., & Sripichitt, P. (2014). Multivariate analysis of genetic diversity among some rice genotypes using morpho-agronomic traits, Journal of Plant Science, 9(1), 14-24. https://scialert.net/fulltext/fulltextpdf.php?pdf=academicjournals/jps/2014/14-24.pdf

Zewdu, Z., Abebe, T., Mitiku, T., Worede, F., Dessie, A., Berie, A., & Atnaf, M. (2020). Performance evaluation and yield stability of upland rice (Oryzasativa L.) varieties in Ethiopia, Cogent Food & Agriculture, 6 (1), 1842679. https://doi.org/10.1080/23311932.2020.1842679

Downloads

Published

28.02.2025

How to Cite

Mekonnen, A. B., & Abebe, T. D. (2025). Morphological Characterization and Multivariate Analysis of Rain Fed Rice (Oryza sativa L) Genotypes in Northwest Ethiopia. Turkish Journal of Agriculture - Food Science and Technology, 13(2), 423–438. https://doi.org/10.24925/turjaf.v13i2.423-438.7274

Issue

Section

Research Paper