Determination of In-Vitro Antifungal Activities of Essential Oils Against Fungal Pathogens
DOI:
https://doi.org/10.24925/turjaf.v12is4.2862-2866.7331Keywords:
Antifungal activity, Essential oil, Fusarium solani, Rhizoctonia solani, PhytopathogenAbstract
This study was carried out to determine the in-vitro antifungal activities of essential oils obtained from thyme (Thymus vulgaris L.), Turkish pickling herb (Echinophora tenuifolia subsp. sibthorpiana), clove (Syzygium aromaticum L.), cinnamon (Cinnamomum zeylanicum Blume), eucalyptus (Eucalyptus globulus Labill), dill (Anethum graveolens L.), juniper (Juniperus communis L.), and ginger (Zingiber officinale Rosc.) against Rhizoctonia solani and Fusarium solani infections. Essential oils were added to autoclaved potato dextrose agar (PDA) medium at 0, 500, 1000 and 2000 ppm doses. Mycelium disks of both fungi with a diameter of 5 mm were transferred to the center of petri dishes and incubated at 24±2 °C for 7 days. The biocontrol efficiencies of the essential oils were calculated by measuring the mycelial development diameters. Essential oil applications showed significant antifungal activity against R. solani pathogen and cinnamon, thyme and clove essential oils at 1000 ppm, and dill essential oil at 2000 ppm doses completely inhibited mycelial development. The biocontrol efficiencies of juniper (2.4-12.6%), eucalyptus (2.8-26.6%) and ginger (18.2-37.3%) essential oils against R. solani were found to be low. While clove and thyme essential oils completely inhibited F. solani mycelial development at 2000 ppm dose, the biocontrol efficiencies of ginger (1.6-3.7%), eucalyptus (1.2-7.4%) and dill (2.9-9.8%) essential oils were low. It was concluded that especially clove, thyme, cinnamon and Turkish pickling herb essential oils showed high in-vitro antifungal activity against both phytopathogens and may have the potential to be used as an alternative to synthetic fungicide active substances.
References
Abd-El-Khair, H., & El-Gamal Nadia, G. (2011). Effects of aqueous extracts of some plant species against Fusarium solani and Rhizoctonia solani in Phaseolus vulgaris plants. Arch. Phytopathol. Plant Protc. 44, 1–16.
Adams, P. R. (2007). Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Carol Stream, IL.
Amini, M., Safaie, N., Salmani, M. J., & Shams-Bakhsh, M. (2012). Antifungal activity of three medicinal plant essential oils against some phytopathogenic fungi. Trakia J. of Sci., 10(1), 1-8.
Arici, S. E., Bozat, G., & Akbulut, I. (2013). Investigation of potantial biological control of Fusarium oxysporum f.sp. radicislycopersici and F. oxysporum f.sp. lycopersici by essential oils, plant extract and chemical elicitors in vitro. Pakistan Journal of Botany, 45, 2119-2124.
Boyraz, N., & Ozcan, M. (2006). Inhibition of phytopathogenic fungi by essential oil, hydrosol, grond material and extract of summer savory Satureja hortensis L. growing wild in Turkey. Int. J. Food Microbiol., 107, 238–242.
Cavanagh, H. M. A. (2007). Antifungal activity of the volatile phase of essential oils. A brief review, Nat. Prod. Commun, 2, 1297–1302.
Çakar, G., Sivrikaya, I. S., Karakaya, E., & Güller, A. (2021). Evaluation of the ın vitro fungicidal activity of summer savory and lavender essential oils against Fusarium solani. ISPEC Journal of Agricultural Sciences, 5(4), 795-805.
Deans, S. G., & Soboda, K. P. (1990). The antimicrobial properties of marjoram (Origanum majorana L.) volatile oil. Flavour Fragr. J., 5, 187-190.
Dorman, H. J. D., & Deans, S. G. (2000). Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. J. Appl. Microbiol, 88, 308–316.
Er, Y. (2018). Ayçiçeği Mildiyösü (Plasmopara halstedii)'ne karşı bazı uçucu yağların antifungal etkilerinin belirlenmesi. Doktora tezi. Ankara Üniversitesi, Bitki Koruma Bölümü.
Erdoğan, O., Çelik, A., Yıldız, Ş., & Kökten, K. (2014). Pamukta fide kök çürüklüğü etmenlerine karşı bazı bitki ekstrakt ve uçucu yağlarının antifungal etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 1(3), 398-404.
European Pharmacopoeia, (1980). Vol. 3, pp. 68. Maisonneuve SA, Sainte Ruffine, France.
Khaledi, N., Taheri, P., & Tarighi, S. (2014). Antifungal activity of various essential oils against Rhizoctonia solani and Macrophomina phaseolina as majör bean pathogens. Journal of Applied Microbiology, 118, 704-717.
Kordali, S., Usanmaz, A., Çakır, A., Komaki, A., & Ercişli, S. (2016). Dört Myrtus communis genotipinin meyve uçucu yağlarının antifungal ve herbisidal etkileri. Kimya ve Biyoçeşitlilik, 13, 77- 84.
Lang, G., & Buchbauer, G. (2012). A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour Fragr. J. 27, 13–39.
Marei, G. I. K. H., & Abdelgaleil, S. A. M. (2018). Antifungal potential and biochemical effects of monoterpenes and phenylpropenes on plant pathogenic fungi. Plant Protect. Sci. 1, 9–16.
Mazid, M., Khan, T. A., & Mohammad, F. (2011). Role of secondary metabolites in defense mechanisms of plant. Biology and Medicine, 3(2), 232-249.
Nazzaro, F., Fratianni, F., Coppola, R., & De Feo, V. (2017). Essential oils and antifungal activity. pharmaceuticals. 10, 86. doi:10.3390/ph10040086
Ntalli, N. G, Ferrari, F, Giannakou, I., & Menkissoglu-Spiroudi, U. (2010). Phytochemistry and nematicidal activity of the essential oils from 8 greek Lamiaceae aromatic plants and 13 terpene components. J. Agric. Food Chem, 58, 7856–7863.
Nwosu, M.O., & Okafor, J. (1995). Preliminary studies of the antifungal activities of some medicinal plants against Basidiobolus and some other pathogenic fungi. Mycoses. 38, 191–195.
Onaran, A., & Yılar, M. (2012). Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. J. Food Agric. Environ. 10, 287–291.
Phillips M. A., & Croteau, R. B. (1999). Resin-based defenses in conifers. Trends Plant Sci., 4, 184–190.
Prabuseenivasan, S., Jayakumar, M., & Ignacimuthu, S. (2006). In vitro antibacterial activity of some plant essential oils, BMC Complementary and Alternative Medicine, 6,39-45.
Ramaroson, M. L., Kutuan, C., Helesbeux, J. J., Clerc, V. L., Hamama, L., Geoffriau, E., & Briard, M. (2022). Role of phenylpropanoids and flavonoids in plant resistance to pests and diseases. Molecules, 27(23), 83-71. doi:org/10.3390/molecules27238371
Rostad, C. E., & Pereira, W. E. 1986. Kovats and Lee retention indices determined by gas chromatography/mass spectrometry for organic compounds of environmental interest. Journal of High Resolution, Chromatography, 9(6), 328-334.
Seema, M., & Devaki N. S. (2012). In vitro evaluation of biological control agents against Rhizoctonia solani. Journal of Agricultural Technology, 8(1), 233-240.
Sharma, A., Rajendran, S., Srivastava, A., Sharma, S., & Kundu, B. (2017). Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil. Journal of Bioscience and Bioengineering, 123(3), 308-313.
Sanli, A., & Ok, F. Z. (2023). Chemical composition and antimicrobial activity against phytopathogenic fungi of essential oils obtained from Echinophora tenuifolia subsp. sibthorpiana grown in wild and cultivated conditions in Turkey. Molecules, 28, 585. https://doi.org/10.3390/molecules28020585
Sivrikaya, I. S., Tosun, B., & Karakaya, E. (2021). Origanum onites L. ve Rosmarinus officinalis L. uçucu yağlarının kimyasal içerikleri ve Fusarium solani’ye karşı antifungal aktivitelerinin belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 8(2), 329-335.
Taweechaisupapong, S., Ngaonee, P., Patsuk, P., Pitiphat, W., & Khunkitti, W. (2012). Antibiofilm activity and post antifungal effect of lemongrass oil on clinical Candida dubliniensis isolate. South Afr. J. Bot., 78, 37–43.
Vaillant, F., Romeu, C., Ramos, R. E., Gonzalez, G. M., Ramirez, O. R., & Gonzalez, P. J. (2009). Inhibitory effect in vitro of five monoterpens from essentials oils on a Rhizoctonia solani isolate in potato (Solanum tuberosum L.). Fitosanidad., 13, 197-200.
Zaidi, M. A., & Crow, S. A. (2005). Biologically active traditional medicinal herbs from Balochistan, Pakistan. J. Ethnopharmacol. 96, 331–334.
Zambonelli, A., D’aulerio, A. Z., Severi, A., Benvenuti, S., Maggi, L., & Bianchi, A. (2004). Chemical composition and fungicidal activity of commercial essential oils of Thymus vulgaris L.. J. Essent Oil Res., 16, 69–74.
Zhang, L., Chen, F., Lai, S., Wang, H., & Yang, H. (2018). Impact of soybean protein isolate-chitosan edible coating on the softening of apricot fruit during storage. LWT-Food Sci. Technol., 96, 604–611.
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