Serological Detection of Three Common Tomato Viruses in Tomato Cultivation Areas of Ereğli District, Konya

Abstract

In this study, it was aimed to reveal some viruses that are a problem in tomato production areas in the Ereğli district of Konya, where tomato production is common. For this reason, in the 2019 production season, leaf samples were taken from 160 tomato plants showing symptoms similar to virus disease symptoms such as mosaic, deformations, yellowing, blight on the leaves and stunted plants. Possible infections of Cucumber mosaic virus (CMV), Tomato spotted-wilt virus (TSWV) and Tomato mosaic virus (ToMV) on these leaves were tested by serological-based DAS-ELISA (Double Antibody Sandwich- Enzyme Linked Immuno Sorbent Assay) method. According to DAS-ELISA results, it was determined that 66.25% of the samples were infected with at least one of these three viruses. In addition, TSWV was detected as the most common virus with a 48.75% relative infection rate in the samples, followed by ToMV (28.75%) and CMV (26.25%). Mixed infections of the viruses were also detected in the leaf samples tested. CMV + TSWV double infection was detected in 22 samples, ToMV + TSWV double infection was detected in 14 samples, and CMV + ToMV + TSWV triple infection was detected in 12 plant samples.