Effect of Heat Treatment, Water and Vinegar Soaking on Protein and Phytic Acid Levels in Hemp Seed Meal
DOI:
https://doi.org/10.24925/turjaf.v12i8.1442-1447.6933Keywords:
feed, antinutritional factor, vinegar, pH, waterAbstract
Hemp plants are notable for their climate resilience, and hempseed meal (HSM) is a potential high-protein feed for poultry. However, HSM has high levels of the antinutritional factor phytic acid (PA). This study aimed to evaluate the effects of heat and soaking treatments on the protein and PA content of HSM. HSM was obtained through cold pressing of whole hempseed and then subjected to heat treatment at 70°C for 24 hours. Soaking treatments involved water, water-vinegar mix, and vinegar for 1, 7, and 24 hours, followed by drying and analysis of PA and protein content. Results indicated that heating increased PA content without affecting protein levels. Soaking duration did not significantly alter protein content but did affect PA levels, with 24-hour soaking significantly increasing PA compared to 1-hour and 7-hour durations. The soaking material also influenced PA content: water soaking increased PA, while a 1-hour vinegar-water mix and 7-hour vinegar soaking significantly reduced PA. The highest PA concentration occurred with 24-hour water soaking. The protein content was highest with 7-hour vinegar soaking. In conclusion, acidic soaking solutions, particularly vinegar and vinegar-water mix, effectively reduced PA in HSM without protein loss.
References
Abrahamsen, F. W., et al. (2021). Effects of feeding varying levels of hempseed meal on dry matter intake, rumen fermentation, in vitro digestibility, blood metabolites, and growth performance of growing meat goats. Applied Animal Science, 37(6), 681-688.
Agume, A. S. N., et al. (2017). Effect of soaking and roasting on the physicochemical and pasting properties of soybean flour. Foods, 6(2), 12.
Albarracín, M., et al. (2013). Effect of soaking process on nutrient bio-accessibility and phytic acid content of brown rice cultivar. LTB-Food Science and Technology, 53(1), 76-80.
Bahari, M., et al. (2014). Effect of Using Medicinal Cannabis Seed (Nigella sativa) in Diets on Performance and Parameters of Broiler Chickens ROSS 308. Advances in Environmental Biology, 8(13), 931-935.
Bohn, L., et al. (2008). Phytate: impact on environment and human nutrition. A challenge for molecular breeding. Journal of Zhejiang University Science B, 9(3), 165-191.
Cheryan, M., & Rackis, J. J. (1980). Phytic acid interactions in food systems. Critical Reviews in Food Science & Nutrition, 13(4), 297-335.
Clarke, R. (2023). Anatolian hemp (Cannabis sativa L.) cultivation, processing, and production: history and recent observations. Agrociencia Uruguay, 27.
Diouf, A., et al. (2020). Improving nutritional quality of cowpea (Vigna unguiculata) by soaking process. International Journal of Food Science and Nutrition Engineering, 10(1), 37-41.
Egli, I., et al. (2002). The influence of soaking and germination on the phytase activity and phytic acid content of grains and seeds potentially useful for complementary feedin. Journal of food science, 67(9), 3484-3488.
Egli, I., et al. (2003). Phytic acid degradation in complementary foods using phytase naturally occurring in whole grain cereals. Journal of food science, 68(5), 1855-1859.
Egounlety, M., & Aworh, O. (2003). Effect of soaking, dehulling, cooking and fermentation with Rhizopus oligosporus on the oligosaccharides, trypsin inhibitor, phytic acid and tannins of soybean (Glycine max Merr.), cowpea (Vigna unguiculata L. Walp) and groundbean (Macrotyloma geocarpa Harms). Journal of food engineering, 56(2-3), 249-254.
Esmaeilipour, O., et al. (2013). The effects of temperature, moisture, duration of incubation time, calcium level, and soaking with water or citric acid on in vitro phytate degradation in a wheat–barley–rye–soybean meal-based diet. Animal Feed Science and Technology, 183(3-4), 168-174.
Feizollahi, E., et al. (2021). Review of the beneficial and anti-nutritional qualities of phytic acid, and procedures for removing it from food products. Food Research International, 143, 110284.
Haug, W., & Lantzsch, H. J. (1983). Sensitive method for the rapid determination of phytate in cereals and cereal products. Journal of the Science of Food and Agriculture, 34(12), 1423-1426.
Hessle, A., et al. (2008). Cold-pressed hempseed cake as a protein feed for growing cattle. Acta Agriculturae Scand Section A, 58(3), 136-145.
Idate, A., et al. (2021). A comprehensive review on antinutritional factors of chickpea (Cicer arietinum L.). The Pharma Innovation Journal, 10(5), 816-823.
Karlsson, L., et al. (2012). Effects of temperature during moist heat treatment on ruminal degradability and intestinal digestibility of protein and amino acids in hempseed cake. Asian-Australasian journal of animal sciences, 25(11), 1559.
Kumar, A., et al. (2021). Phytic acid: Blessing in disguise, a prime compound required for both plant and human nutrition. Food Research International, 142, 110193.
Lestienne, I., et al. (2005a). Iron and zinc in vitro availability in pearl millet flours (Pennisetum glaucum) with varying phytate, tannin, and fiber contents. Journal of Agricultural and Food Chemistry, 53(8), 3240-3247.
Lestienne, I., et al. (2005b). Effects of soaking whole cereal and legume seeds on iron, zinc and phytate contents. Food chemistry, 89(3), 421-425.
Liang, J., et al. (2008). Effects of soaking, germination and fermentation on phytic acid, total and in vitro soluble zinc in brown rice. Food chemistry, 110(4), 821-828.
Luo, Y., et al. (2009). Impact of soaking and phytase treatments on phytic acid, calcium, iron and zinc in faba bean fractions. International journal of food science & technology, 44(12), 2590-2597.
Mahmoudi, M., et al. (2015). Effects of different levels of hemp seed (Cannabis sativa L.) and dextran oligosaccharide on growth performance and antibody titer response of broiler chickens. Italian Journal of Animal Science, 14(1), 3473.
Mustafa, A., et al. (1999). The nutritive value of hemp meal for ruminants. Canadian Journal of Animal Science, 79(1), 91-95.
Oatway, L., et al. (2001). Phytic acid. Food Reviews International, 17(4), 419-431.
Ondrej, S., et al. (2015). The effect of hempseed cakes on broiler chickens peroformance parameters. Mendelnet, 1(1), 157-160.
Qureshi, S., & Asmaahamid. (2020). Effect of overnight soaking and boiling on phytic acid, tannins, saponins and proximate composition in legumes. Journal of Pure and Applied Agriculture, 5(3), 17–25.
Rasha Mohamed, K., et al. (2011). Effect of legume processing treatments individually or in combination on their phytic acid content. African Journal Food Science Technolgy, 2(2), 036-046.
Russo, R., & Reggiani, R. (2015). Evaluation of protein concentration, amino acid profile and antinutritional compounds in hempseed meal from dioecious and monoecious varieties. American Journal of Plant Sciences, 6(01), 14.
Shariatmadari, F. (2023). Emergence of hemp as feed for poultry. World’s Poultry Science Journal, 79(4), 769-782.
Shi, L., et al. (2018). Changes in levels of phytic acid, lectins and oxalates during soaking and cooking of Canadian pulses. Food Research International, 107, 660-668.
Vötterl, J., et al. (2019). Soaking in lactic acid lowers the phytate-phosphorus content and increases the resistant starch in wheat and corn grains. Animal Feed Science and Technology, 252, 115-125.
Xu, Y., et al. (2021). Hempseed as a nutritious and healthy human food or animal feed source: a review. International journal of food science & technology, 56(2), 530-543.
Yoshida, T., et al. (1975). Phytase activity associated with isolated aleurone particles of rice grains. Agricultural and Biological Chemistry, 39(1), 289-290.
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