Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’da Antioksidan Enzim Aktivitesi Üzerine Etkisi

Yazarlar

DOI:

https://doi.org/10.24925/turjaf.v12i3.447-452.6624

Anahtar Kelimeler:

NaCl- arjinin- SOD- POD- APX- QPCR

Özet

Arjinin, bitkilerde stres metabolizmasında yer alan çeşitli metabolitlerin öncülüğünü yapan bir aminoasit olmasının yanı sıra, bitkilerin çevresel streslere yanıtını modüle edebilen bir sinyal molekülüdür. Tuzluluk stresi, özellikle mısır (Zea mays) yetiştiriciliğinde, mahsul verimliliğini artırma açısından önemli bir zorluk oluşturmaktadır. Bu çalışma, L-argininin (L-arg) lipid peroksidasyonunu, bazı antioksidan enzim aktivitelerini ve bu enzimlerin gen ifade seviyelerini modüle ederek tuz stresinin neden olduğu oksidatif hasarı hafifletmedeki potansiyel rolünü araştırmaktadır. Bulgularımız, 200 mM tuz stresi uygulamasında H2O2 ve MDA seviyelerinin arttığını, ancak 1,5 ve 3 mM L-arg uygulamalarının bu seviyeleri azalttığını göstermektedir. Ayrıca çalışmamızda, süperoksit dismutaz (SOD), peroksidaz (POD) ve askorbat peroksidaz (APX) aktiviteleri ile ZmSOD, ZmPOD ve ZmAPX gen ifade seviyelerinin tuz stresi altında giderek arttığı, ancak L-arg’ın bu parametreleri arttırmada daha etkili olduğu belirlenmiştir. En yüksek artışlar SOD enzim aktivitesinde ve ZmSOD gen ifadesinde gözlemlenmiştir. Bu araştırma, tuz stresinde bazı moleküler ve biyokimyasal yanıtların anlaşılmasını derinleştirerek, L-arg uygulamasının bitkilerin çevresel zorluklara karşı direncini artırmada kritik bilgiler sunabileceği önemli bir literatür kaynağıdır.

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Yayınlanmış

2024-03-23

Nasıl Atıf Yapılır

Arslan Yüksel, E. (2024). Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’da Antioksidan Enzim Aktivitesi Üzerine Etkisi. Türk Tarım - Gıda Bilim Ve Teknoloji Dergisi, 12(3), 447–452. https://doi.org/10.24925/turjaf.v12i3.447-452.6624

Sayı

Bölüm

Araştırma Makalesi